Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands
<i>Salmonella</i> Infantis, a common contaminant of poultry products, is known to harbor mobile genetic elements that confer multi-drug resistance (MDR) and have been detected in many continents. Here, we report four MDR <i>S</i>. <i>Infantis</i> strains recovered...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/3/267 |
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| author | Elton Burnett Maria Ishida Sofia de Janon Sohail Naushad Marc-Olivier Duceppe Ruimin Gao Armando Jardim Jessica C. Chen Kaitlin A. Tagg Dele Ogunremi Christian Vinueza-Burgos |
| author_facet | Elton Burnett Maria Ishida Sofia de Janon Sohail Naushad Marc-Olivier Duceppe Ruimin Gao Armando Jardim Jessica C. Chen Kaitlin A. Tagg Dele Ogunremi Christian Vinueza-Burgos |
| author_sort | Elton Burnett |
| collection | DOAJ |
| description | <i>Salmonella</i> Infantis, a common contaminant of poultry products, is known to harbor mobile genetic elements that confer multi-drug resistance (MDR) and have been detected in many continents. Here, we report four MDR <i>S</i>. <i>Infantis</i> strains recovered from poultry house environments in Santa Cruz Island of the Galapagos showing extended-spectrum β-lactamase (ESBL) resistance and reduced fluoroquinolone susceptibility. Whole-genome sequencing (WGS) revealed the presence of the ESBL-conferring <i>bla</i><sub>CTX-M-65</sub> gene in an IncFIB-like plasmid in three <i>S</i>. Infantis isolates. Multi-locus sequence typing (MLST) and single nucleotide variant/polymorphism (SNP) SNVPhyl analysis showed that the <i>S</i>. Infantis isolates belong to sequence type ST32, likely share a common ancestor, and are closely related (1–3 SNP difference) to <i>bla</i><sub>CTX-M-65</sub>-containing clinical and veterinary <i>S</i>. Infantis isolates from the United States and Latin America. Furthermore, phylogenetic analysis of SNPs following core-genome alignment (i.e., ParSNP) inferred close relatedness between the <i>S</i>. Infantis isolates from Galapagos and the United States. Prophage typing confirmed the close relationship among the Galapagos <i>S</i>. Infantis and was useful in distinguishing them from the United States isolates. This is the first report of MDR <i>bla</i><sub>CTX-M-65</sub>-containing <i>S</i>. Infantis in the Galapagos Islands and highlights the need for increased monitoring and surveillance programs to determine prevalence, sources, and reservoirs of MDR pathogens. |
| first_indexed | 2024-03-09T05:22:45Z |
| format | Article |
| id | doaj.art-fd09078661c547a5bf3d3942037ed012 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T05:22:45Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-fd09078661c547a5bf3d3942037ed0122023-12-03T12:39:42ZengMDPI AGAntibiotics2079-63822021-03-0110326710.3390/antibiotics10030267Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos IslandsElton Burnett0Maria Ishida1Sofia de Janon2Sohail Naushad3Marc-Olivier Duceppe4Ruimin Gao5Armando Jardim6Jessica C. Chen7Kaitlin A. Tagg8Dele Ogunremi9Christian Vinueza-Burgos10Institute of Parasitology, McGill University, Montreal, QC H9X 3V9, CanadaDivision of Food Laboratory, New York State Department of Agriculture and Markets, Albany, NY 12206, USAUnidad de investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito 170103, EcuadorOttawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON K2J 4S1, CanadaOttawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON K2J 4S1, CanadaOttawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON K2J 4S1, CanadaInstitute of Parasitology, McGill University, Montreal, QC H9X 3V9, CanadaDivision of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USADivision of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USAOttawa Laboratory Fallowfield, Canadian Food Inspection Agency, Ottawa, ON K2J 4S1, CanadaUnidad de investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito 170103, Ecuador<i>Salmonella</i> Infantis, a common contaminant of poultry products, is known to harbor mobile genetic elements that confer multi-drug resistance (MDR) and have been detected in many continents. Here, we report four MDR <i>S</i>. <i>Infantis</i> strains recovered from poultry house environments in Santa Cruz Island of the Galapagos showing extended-spectrum β-lactamase (ESBL) resistance and reduced fluoroquinolone susceptibility. Whole-genome sequencing (WGS) revealed the presence of the ESBL-conferring <i>bla</i><sub>CTX-M-65</sub> gene in an IncFIB-like plasmid in three <i>S</i>. Infantis isolates. Multi-locus sequence typing (MLST) and single nucleotide variant/polymorphism (SNP) SNVPhyl analysis showed that the <i>S</i>. Infantis isolates belong to sequence type ST32, likely share a common ancestor, and are closely related (1–3 SNP difference) to <i>bla</i><sub>CTX-M-65</sub>-containing clinical and veterinary <i>S</i>. Infantis isolates from the United States and Latin America. Furthermore, phylogenetic analysis of SNPs following core-genome alignment (i.e., ParSNP) inferred close relatedness between the <i>S</i>. Infantis isolates from Galapagos and the United States. Prophage typing confirmed the close relationship among the Galapagos <i>S</i>. Infantis and was useful in distinguishing them from the United States isolates. This is the first report of MDR <i>bla</i><sub>CTX-M-65</sub>-containing <i>S</i>. Infantis in the Galapagos Islands and highlights the need for increased monitoring and surveillance programs to determine prevalence, sources, and reservoirs of MDR pathogens.https://www.mdpi.com/2079-6382/10/3/267<i>Salmonella</i> Infantismulti-drug resistanceGalapagosextended-spectrum β-lactamase |
| spellingShingle | Elton Burnett Maria Ishida Sofia de Janon Sohail Naushad Marc-Olivier Duceppe Ruimin Gao Armando Jardim Jessica C. Chen Kaitlin A. Tagg Dele Ogunremi Christian Vinueza-Burgos Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands Antibiotics <i>Salmonella</i> Infantis multi-drug resistance Galapagos extended-spectrum β-lactamase |
| title | Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands |
| title_full | Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands |
| title_fullStr | Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands |
| title_full_unstemmed | Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands |
| title_short | Whole-Genome Sequencing Reveals the Presence of the <i>bla</i><sub>CTX-M-65</sub> Gene in Extended-Spectrum β-Lactamase-Producing and Multi-Drug-Resistant Clones of <i>Salmonella</i> Serovar Infantis Isolated from Broiler Chicken Environments in the Galapagos Islands |
| title_sort | whole genome sequencing reveals the presence of the i bla i sub ctx m 65 sub gene in extended spectrum β lactamase producing and multi drug resistant clones of i salmonella i serovar infantis isolated from broiler chicken environments in the galapagos islands |
| topic | <i>Salmonella</i> Infantis multi-drug resistance Galapagos extended-spectrum β-lactamase |
| url | https://www.mdpi.com/2079-6382/10/3/267 |
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