3D imaging using scanning diffractometry
Abstract Imaging of cells is a challenging problem as they do not appreciably change the intensity of the illuminating light. Interferometry-based methods to do this task suffer from high sensitivity to environmental vibrations. We introduce scanning diffractometry as a simple non-contact and vibrat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79939-y |
| _version_ | 1818682978975350784 |
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| author | Morteza J. Siavashani Iman Naghedi Vahid Abbasian Ehsan A. Akhlaghi Mohammad A. Charsooghi Mohammad Taghi Tavassoly Ali-Reza Moradi |
| author_facet | Morteza J. Siavashani Iman Naghedi Vahid Abbasian Ehsan A. Akhlaghi Mohammad A. Charsooghi Mohammad Taghi Tavassoly Ali-Reza Moradi |
| author_sort | Morteza J. Siavashani |
| collection | DOAJ |
| description | Abstract Imaging of cells is a challenging problem as they do not appreciably change the intensity of the illuminating light. Interferometry-based methods to do this task suffer from high sensitivity to environmental vibrations. We introduce scanning diffractometry as a simple non-contact and vibration-immune methodology for quantitative phase imaging. Fresnel diffractometry by a phase step has led to several applications such as high-precision measurements of displacement. Additional scanning may lead to 3D imaging straightforwardly. We apply the technique to acquire 3D images of holographic grating, red blood cell, neuron, and sperm cell. Either visibility of the diffraction fringes or the positions of extrema may be used for phase change detection. The theoretical analysis through the Fresnel diffraction from one-dimensional phase step is presented and the experimental results are validated with digital holographic microscopy. The presented technique can be suggested to serve as a robust device for 3D phase imaging and biomedical measurements. |
| first_indexed | 2024-12-17T10:27:26Z |
| format | Article |
| id | doaj.art-fd0b065a8be6445eaa31eb20c4039d96 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T10:27:26Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fd0b065a8be6445eaa31eb20c4039d962022-12-21T21:52:37ZengNature PortfolioScientific Reports2045-23222021-01-0111111010.1038/s41598-020-79939-y3D imaging using scanning diffractometryMorteza J. Siavashani0Iman Naghedi1Vahid Abbasian2Ehsan A. Akhlaghi3Mohammad A. Charsooghi4Mohammad Taghi Tavassoly5Ali-Reza Moradi6Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS)Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS)Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS)Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS)Department of Physics, Institute for Advanced Studies in Basic Sciences (IASBS)Department of Physics, College of Science, University of TehranDepartment of Physics, Institute for Advanced Studies in Basic Sciences (IASBS)Abstract Imaging of cells is a challenging problem as they do not appreciably change the intensity of the illuminating light. Interferometry-based methods to do this task suffer from high sensitivity to environmental vibrations. We introduce scanning diffractometry as a simple non-contact and vibration-immune methodology for quantitative phase imaging. Fresnel diffractometry by a phase step has led to several applications such as high-precision measurements of displacement. Additional scanning may lead to 3D imaging straightforwardly. We apply the technique to acquire 3D images of holographic grating, red blood cell, neuron, and sperm cell. Either visibility of the diffraction fringes or the positions of extrema may be used for phase change detection. The theoretical analysis through the Fresnel diffraction from one-dimensional phase step is presented and the experimental results are validated with digital holographic microscopy. The presented technique can be suggested to serve as a robust device for 3D phase imaging and biomedical measurements.https://doi.org/10.1038/s41598-020-79939-y |
| spellingShingle | Morteza J. Siavashani Iman Naghedi Vahid Abbasian Ehsan A. Akhlaghi Mohammad A. Charsooghi Mohammad Taghi Tavassoly Ali-Reza Moradi 3D imaging using scanning diffractometry Scientific Reports |
| title | 3D imaging using scanning diffractometry |
| title_full | 3D imaging using scanning diffractometry |
| title_fullStr | 3D imaging using scanning diffractometry |
| title_full_unstemmed | 3D imaging using scanning diffractometry |
| title_short | 3D imaging using scanning diffractometry |
| title_sort | 3d imaging using scanning diffractometry |
| url | https://doi.org/10.1038/s41598-020-79939-y |
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