Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis.
In flowering plants, self-incompatibility is an effective genetic mechanism that prevents self-fertilization. Most Prunus tree species exhibit a homomorphic gametophytic self-incompatibility (GSI) system, in which the pollen phenotype is encoded by its own haploid genome. To date, no identification...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4922633?pdf=render |
| _version_ | 1818989725335158784 |
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| author | Judith Ssali Nantongo Gerald Eilu Thomas Geburek Silvio Schueler Heino Konrad |
| author_facet | Judith Ssali Nantongo Gerald Eilu Thomas Geburek Silvio Schueler Heino Konrad |
| author_sort | Judith Ssali Nantongo |
| collection | DOAJ |
| description | In flowering plants, self-incompatibility is an effective genetic mechanism that prevents self-fertilization. Most Prunus tree species exhibit a homomorphic gametophytic self-incompatibility (GSI) system, in which the pollen phenotype is encoded by its own haploid genome. To date, no identification of S-alleles had been done in Prunus africana, the only member of the genus in Africa. To identify S-RNase alleles and hence determine S-genotypes in African cherry (Prunus africana) from Mabira Forest Reserve, Uganda, primers flanking the first and second intron were designed and these amplified two bands in most individuals. PCR bands on agarose indicated 26 and 8 different S-alleles for second and first intron respectively. Partial or full sequences were obtained for all these fragments. Comparison with published S-RNase data indicated that the amplified products were S-RNase alleles with very high interspecies homology despite the high intraspecific variation. Against expectations for a locus under balancing selection, frequency and spatial distribution of the alleles in a study plot was not random. Implications of the results to breeding efforts in the species are discussed, and mating experiments are strongly suggested to finally prove the functionality of SI in P. africana. |
| first_indexed | 2024-12-20T19:43:02Z |
| format | Article |
| id | doaj.art-fd0b1dd6203042d2b95b65e3ea2a94f7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T19:43:02Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fd0b1dd6203042d2b95b65e3ea2a94f72022-12-21T19:28:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01116e015563810.1371/journal.pone.0155638Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis.Judith Ssali NantongoGerald EiluThomas GeburekSilvio SchuelerHeino KonradIn flowering plants, self-incompatibility is an effective genetic mechanism that prevents self-fertilization. Most Prunus tree species exhibit a homomorphic gametophytic self-incompatibility (GSI) system, in which the pollen phenotype is encoded by its own haploid genome. To date, no identification of S-alleles had been done in Prunus africana, the only member of the genus in Africa. To identify S-RNase alleles and hence determine S-genotypes in African cherry (Prunus africana) from Mabira Forest Reserve, Uganda, primers flanking the first and second intron were designed and these amplified two bands in most individuals. PCR bands on agarose indicated 26 and 8 different S-alleles for second and first intron respectively. Partial or full sequences were obtained for all these fragments. Comparison with published S-RNase data indicated that the amplified products were S-RNase alleles with very high interspecies homology despite the high intraspecific variation. Against expectations for a locus under balancing selection, frequency and spatial distribution of the alleles in a study plot was not random. Implications of the results to breeding efforts in the species are discussed, and mating experiments are strongly suggested to finally prove the functionality of SI in P. africana.http://europepmc.org/articles/PMC4922633?pdf=render |
| spellingShingle | Judith Ssali Nantongo Gerald Eilu Thomas Geburek Silvio Schueler Heino Konrad Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis. PLoS ONE |
| title | Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis. |
| title_full | Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis. |
| title_fullStr | Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis. |
| title_full_unstemmed | Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis. |
| title_short | Detection of Self Incompatibility Genotypes in Prunus africana: Characterization, Evolution and Spatial Analysis. |
| title_sort | detection of self incompatibility genotypes in prunus africana characterization evolution and spatial analysis |
| url | http://europepmc.org/articles/PMC4922633?pdf=render |
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