Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection
The rhizosphere community represents an “ecological interface” between plant and soil, providing the plant with a number of advantages. Despite close connection and mutual influence in this system, the knowledge about the connection of plant and rhizosphere diversity is still controversial. One of t...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2339 |
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| author | Aleksei O. Zverev Arina A. Kichko Aleksandr G. Pinaev Nikolay A. Provorov Evgeny E. Andronov |
| author_facet | Aleksei O. Zverev Arina A. Kichko Aleksandr G. Pinaev Nikolay A. Provorov Evgeny E. Andronov |
| author_sort | Aleksei O. Zverev |
| collection | DOAJ |
| description | The rhizosphere community represents an “ecological interface” between plant and soil, providing the plant with a number of advantages. Despite close connection and mutual influence in this system, the knowledge about the connection of plant and rhizosphere diversity is still controversial. One of the most valuable factors of this uncertainty is a rough estimation of plant diversity. NGS sequencing can make the estimations of the plant community more precise than classical geobotanical methods. We investigate fallow and crop sites, which are similar in terms of environmental conditions and soil legacy, yet at the same time are significantly different in terms of plant diversity. We explored amplicons of both the plant root mass (ITS1 DNA) and the microbial communities (16S rDNA); determined alpha- and beta-diversity indices and their correlation, and performed differential abundance analysis. In the analysis, there is no correlation between the alpha-diversity indices of plants and the rhizosphere microbial communities. The beta-diversity between rhizosphere microbial communities and plant communities is highly correlated (R = 0.866, <i>p</i> = 0.01). ITS1 sequencing is effective for the description of plant root communities. There is a connection between rhizosphere communities and the composition of plants, but on the alpha-diversity level we found no correlation. In the future, the connection of alpha-diversities should be explored using ITS1 sequencing, even in more similar plant communities—for example, in different synusia. |
| first_indexed | 2024-03-10T05:15:22Z |
| format | Article |
| id | doaj.art-fd0ba75f431941c795eba42fc221ee3e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:22Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fd0ba75f431941c795eba42fc221ee3e2023-11-23T00:29:56ZengMDPI AGMicroorganisms2076-26072021-11-01911233910.3390/microorganisms9112339Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the ConnectionAleksei O. Zverev0Arina A. Kichko1Aleksandr G. Pinaev2Nikolay A. Provorov3Evgeny E. Andronov4All-Russian Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chaussee 3, 196608 St. Petersburg, RussiaAll-Russian Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chaussee 3, 196608 St. Petersburg, RussiaAll-Russian Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chaussee 3, 196608 St. Petersburg, RussiaAll-Russian Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chaussee 3, 196608 St. Petersburg, RussiaAll-Russian Research Institute for Agricultural Microbiology (ARRIAM), Podbelsky Chaussee 3, 196608 St. Petersburg, RussiaThe rhizosphere community represents an “ecological interface” between plant and soil, providing the plant with a number of advantages. Despite close connection and mutual influence in this system, the knowledge about the connection of plant and rhizosphere diversity is still controversial. One of the most valuable factors of this uncertainty is a rough estimation of plant diversity. NGS sequencing can make the estimations of the plant community more precise than classical geobotanical methods. We investigate fallow and crop sites, which are similar in terms of environmental conditions and soil legacy, yet at the same time are significantly different in terms of plant diversity. We explored amplicons of both the plant root mass (ITS1 DNA) and the microbial communities (16S rDNA); determined alpha- and beta-diversity indices and their correlation, and performed differential abundance analysis. In the analysis, there is no correlation between the alpha-diversity indices of plants and the rhizosphere microbial communities. The beta-diversity between rhizosphere microbial communities and plant communities is highly correlated (R = 0.866, <i>p</i> = 0.01). ITS1 sequencing is effective for the description of plant root communities. There is a connection between rhizosphere communities and the composition of plants, but on the alpha-diversity level we found no correlation. In the future, the connection of alpha-diversities should be explored using ITS1 sequencing, even in more similar plant communities—for example, in different synusia.https://www.mdpi.com/2076-2607/9/11/2339diversity correlationalpha-diversity of plantsITS1 plants sequencingcrop rhizosphere16S and ITS1 sequencing |
| spellingShingle | Aleksei O. Zverev Arina A. Kichko Aleksandr G. Pinaev Nikolay A. Provorov Evgeny E. Andronov Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection Microorganisms diversity correlation alpha-diversity of plants ITS1 plants sequencing crop rhizosphere 16S and ITS1 sequencing |
| title | Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection |
| title_full | Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection |
| title_fullStr | Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection |
| title_full_unstemmed | Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection |
| title_short | Diversity Indices of Plant Communities and Their Rhizosphere Microbiomes: An Attempt to Find the Connection |
| title_sort | diversity indices of plant communities and their rhizosphere microbiomes an attempt to find the connection |
| topic | diversity correlation alpha-diversity of plants ITS1 plants sequencing crop rhizosphere 16S and ITS1 sequencing |
| url | https://www.mdpi.com/2076-2607/9/11/2339 |
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