Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury
Testicular torsion, a common urologic emergency, is primarily caused by ischemia/reperfusion (I/R) injury of the testis. Nitric oxide (NO)-derived from nitrite (NO2−) has been reported to have prominent therapeutic effects on I/R injury in the heart, liver, and brain; however, its effects on testicu...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Asian Journal of Andrology |
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| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=1;spage=92;epage=97;aulast=Lee |
| _version_ | 1828336902813515776 |
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| author | Jae Won Lee Dong-Hun Lee Jae Keun Park Jin Soo Han |
| author_facet | Jae Won Lee Dong-Hun Lee Jae Keun Park Jin Soo Han |
| author_sort | Jae Won Lee |
| collection | DOAJ |
| description | Testicular torsion, a common urologic emergency, is primarily caused by ischemia/reperfusion (I/R) injury of the testis. Nitric oxide (NO)-derived from nitrite (NO2−) has been reported to have prominent therapeutic effects on I/R injury in the heart, liver, and brain; however, its effects on testicular I/R injury have not been evaluated. This study, therefore, investigated whether NO from NO2− is beneficial in a rat model of testicular I/R injury which eventually results in impaired spermatogenesis. Male Sprague-Dawley rats were assigned to the following seven groups: group A, sham-operated control group; Group B, I/R with no treatment; Groups C, D, and E, I/R followed by treatment with three different doses of NO2−; Group F, I/R followed by administration of NO2− and NO scavenger (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt [C-PTIO]); and Group G, I/R followed by administration of nitrate (NO3−). NO2−, NO3−, and C-PTIO were intravenously administered. Histological examination of the testes and the western blot analysis of caspase-3 were performed. Levels of antioxidant enzymes and lipid peroxidation were measured. Germ cell apoptosis, oxidative stress, antioxidant enzymatic function, and lipid peroxidation in Group B were significantly higher than those in Group A. Group B exhibited an abnormal testicular morphology and impaired spermatogenesis. In contrast, testicular damages were attenuated in the NO2− treatment groups, which were caused by reduction in superoxide and peroxynitrite levels and an inhibition of caspase-3-dependent apoptosis. The results of this study suggest NO2− to be a promising therapeutic agent with anti-oxidant and anti-apoptotic properties in testicular I/R injury. |
| first_indexed | 2024-04-13T22:07:41Z |
| format | Article |
| id | doaj.art-fd0d39e25f35420cae47d84d9c67fb46 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-04-13T22:07:41Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-fd0d39e25f35420cae47d84d9c67fb462022-12-22T02:27:53ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622019-01-01211929710.4103/aja.aja_76_18Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injuryJae Won LeeDong-Hun LeeJae Keun ParkJin Soo HanTesticular torsion, a common urologic emergency, is primarily caused by ischemia/reperfusion (I/R) injury of the testis. Nitric oxide (NO)-derived from nitrite (NO2−) has been reported to have prominent therapeutic effects on I/R injury in the heart, liver, and brain; however, its effects on testicular I/R injury have not been evaluated. This study, therefore, investigated whether NO from NO2− is beneficial in a rat model of testicular I/R injury which eventually results in impaired spermatogenesis. Male Sprague-Dawley rats were assigned to the following seven groups: group A, sham-operated control group; Group B, I/R with no treatment; Groups C, D, and E, I/R followed by treatment with three different doses of NO2−; Group F, I/R followed by administration of NO2− and NO scavenger (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt [C-PTIO]); and Group G, I/R followed by administration of nitrate (NO3−). NO2−, NO3−, and C-PTIO were intravenously administered. Histological examination of the testes and the western blot analysis of caspase-3 were performed. Levels of antioxidant enzymes and lipid peroxidation were measured. Germ cell apoptosis, oxidative stress, antioxidant enzymatic function, and lipid peroxidation in Group B were significantly higher than those in Group A. Group B exhibited an abnormal testicular morphology and impaired spermatogenesis. In contrast, testicular damages were attenuated in the NO2− treatment groups, which were caused by reduction in superoxide and peroxynitrite levels and an inhibition of caspase-3-dependent apoptosis. The results of this study suggest NO2− to be a promising therapeutic agent with anti-oxidant and anti-apoptotic properties in testicular I/R injury.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=1;spage=92;epage=97;aulast=Leeischemia/reperfusion injury; nitric oxide; nitrite; rat; testis |
| spellingShingle | Jae Won Lee Dong-Hun Lee Jae Keun Park Jin Soo Han Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury Asian Journal of Andrology ischemia/reperfusion injury; nitric oxide; nitrite; rat; testis |
| title | Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury |
| title_full | Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury |
| title_fullStr | Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury |
| title_full_unstemmed | Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury |
| title_short | Sodium nitrite-derived nitric oxide protects rat testes against ischemia/reperfusion injury |
| title_sort | sodium nitrite derived nitric oxide protects rat testes against ischemia reperfusion injury |
| topic | ischemia/reperfusion injury; nitric oxide; nitrite; rat; testis |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2019;volume=21;issue=1;spage=92;epage=97;aulast=Lee |
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