Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood
The activity of urease purified from Proteus mirabilis bacteria wasestimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained asstandard in size (20- 40)nm, purity 99%. The enzyme activity was estimated byincubating the pure enzyme with various concentrations of nanoparticles range...
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| Format: | Article |
| Language: | English |
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Unviversity of Technology- Iraq
2018-12-01
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| Series: | Engineering and Technology Journal |
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| Online Access: | https://etj.uotechnology.edu.iq/article_175481_c2416c9adc539d27daeaae2e13563fab.pdf |
| _version_ | 1827358844645277696 |
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| author | Hadeel Dawud Entesar Ali |
| author_facet | Hadeel Dawud Entesar Ali |
| author_sort | Hadeel Dawud |
| collection | DOAJ |
| description | The activity of urease purified from Proteus mirabilis bacteria wasestimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained asstandard in size (20- 40)nm, purity 99%. The enzyme activity was estimated byincubating the pure enzyme with various concentrations of nanoparticles rangedbetween (1- 6) μg/ml. The results indicates a decrease in enzymatic activity withincreasing of nanoparticles concentration. After that, the concentration of bloodurea was measured using urease obtained from a standard kit, urease purified fromProteus mirabilis and the urease- gamma iron nanoparticles solution. Thecomparison was then made among the results of urea concentrations by statisticalanalysis using T-test. The results showed that there is no significant differencebetween the results obtained from urease standard kit and urease purified fromthe bacteria, this is due to the efficiency of urease purified from the bacteria. On theother hand, the results showed that there is a significant difference (P≤ 0.01) inurea concentrations obtained from urease- gamma iron oxide nanoparticlessolution due to the inhibition of the enzyme which lost its activity by nanoparticles. |
| first_indexed | 2024-03-08T06:17:03Z |
| format | Article |
| id | doaj.art-fd1089078e954d30a4a78abcc8ea0f37 |
| institution | Directory Open Access Journal |
| issn | 1681-6900 2412-0758 |
| language | English |
| last_indexed | 2024-03-08T06:17:03Z |
| publishDate | 2018-12-01 |
| publisher | Unviversity of Technology- Iraq |
| record_format | Article |
| series | Engineering and Technology Journal |
| spelling | doaj.art-fd1089078e954d30a4a78abcc8ea0f372024-02-04T17:20:11ZengUnviversity of Technology- IraqEngineering and Technology Journal1681-69002412-07582018-12-01362B15215510.30684/etj.36.2B.10175481Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in bloodHadeel Dawud0Entesar Ali1Applied Sciences Department, University of Technology, Baghdad, Iraq.Applied Sciences Department, University of Technology, Baghdad, IraqThe activity of urease purified from Proteus mirabilis bacteria wasestimated using gamma iron oxide nanoparticles (γ-Fe2O3) which were obtained asstandard in size (20- 40)nm, purity 99%. The enzyme activity was estimated byincubating the pure enzyme with various concentrations of nanoparticles rangedbetween (1- 6) μg/ml. The results indicates a decrease in enzymatic activity withincreasing of nanoparticles concentration. After that, the concentration of bloodurea was measured using urease obtained from a standard kit, urease purified fromProteus mirabilis and the urease- gamma iron nanoparticles solution. Thecomparison was then made among the results of urea concentrations by statisticalanalysis using T-test. The results showed that there is no significant differencebetween the results obtained from urease standard kit and urease purified fromthe bacteria, this is due to the efficiency of urease purified from the bacteria. On theother hand, the results showed that there is a significant difference (P≤ 0.01) inurea concentrations obtained from urease- gamma iron oxide nanoparticlessolution due to the inhibition of the enzyme which lost its activity by nanoparticles.https://etj.uotechnology.edu.iq/article_175481_c2416c9adc539d27daeaae2e13563fab.pdfproteus mirabilisureaseiron oxide nanoparticles |
| spellingShingle | Hadeel Dawud Entesar Ali Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood Engineering and Technology Journal proteus mirabilis urease iron oxide nanoparticles |
| title | Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood |
| title_full | Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood |
| title_fullStr | Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood |
| title_full_unstemmed | Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood |
| title_short | Evaluation of purified urease activity from Proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood |
| title_sort | evaluation of purified urease activity from proteus mirabilis using iron oxide nanoparticles and measurement of urea concentration in blood |
| topic | proteus mirabilis urease iron oxide nanoparticles |
| url | https://etj.uotechnology.edu.iq/article_175481_c2416c9adc539d27daeaae2e13563fab.pdf |
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