The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development
Background/ Aims: The knowledge of the molecular network that governs fetal lung branching is an essential step towards the discovery of novel therapeutic targets against pulmonary pathologies. Lung consists of two highly branched systems: airways and vasculature. Ephrins and its receptors, Eph, hav...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2015-01-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/369679 |
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| author | Francisca O. Peixoto Patrícia Pereira-Terra Rute S. Moura Emanuel Carvalho-Dias Jorge Correia-Pinto Cristina Nogueira-Silva |
| author_facet | Francisca O. Peixoto Patrícia Pereira-Terra Rute S. Moura Emanuel Carvalho-Dias Jorge Correia-Pinto Cristina Nogueira-Silva |
| author_sort | Francisca O. Peixoto |
| collection | DOAJ |
| description | Background/ Aims: The knowledge of the molecular network that governs fetal lung branching is an essential step towards the discovery of novel therapeutic targets against pulmonary pathologies. Lung consists of two highly branched systems: airways and vasculature. Ephrins and its receptors, Eph, have been implicated in cardiovascular development, angiogenesis and vascular remodeling. This study aims to clarify the role of these factors during lung morphogenesis. Methods: Ephrins-B1, -B2 and receptor EphB4 expression pattern was assessed in fetal rat lungs between 15.5 and 21.5 days post-conception, by immunohistochemistry. Fetal rat lungs were harvested at 13.5 dpc, cultured during 4 days and treated with increasing doses of ephrins-B1 and -B2 and the activity of key signaling pathways was assessed. Results: Ephrin-B1 presents mesenchymal expression, whereas ephrin-B2 and its receptor EphB4 were expressed by the epithelium. Both ephrins stimulated pulmonary branching. Moreover, while ephrin-B1 did not affect the pathways studied, ephrin-B2 supplementation decreased activity of JNK, ERK and STAT. This study characterizes the expression pattern of ephrins-B1, -B2 and EphB4 receptor throughout rat lung development. Conclusion: Our data highlight a possible role of ephrins as molecular stimulators of lung morphogenesis. Moreover, it supports the idea that classical vascular factors might play a role as airway growth promoters. |
| first_indexed | 2024-04-13T05:17:04Z |
| format | Article |
| id | doaj.art-fd10f0f33fdb4aaf8f366f09defe0222 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-04-13T05:17:04Z |
| publishDate | 2015-01-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-fd10f0f33fdb4aaf8f366f09defe02222022-12-22T03:00:52ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-01-0135110411510.1159/000369679369679The Role of Ephrins-B1 and -B2 During Fetal Rat Lung DevelopmentFrancisca O. PeixotoPatrícia Pereira-TerraRute S. MouraEmanuel Carvalho-DiasJorge Correia-PintoCristina Nogueira-SilvaBackground/ Aims: The knowledge of the molecular network that governs fetal lung branching is an essential step towards the discovery of novel therapeutic targets against pulmonary pathologies. Lung consists of two highly branched systems: airways and vasculature. Ephrins and its receptors, Eph, have been implicated in cardiovascular development, angiogenesis and vascular remodeling. This study aims to clarify the role of these factors during lung morphogenesis. Methods: Ephrins-B1, -B2 and receptor EphB4 expression pattern was assessed in fetal rat lungs between 15.5 and 21.5 days post-conception, by immunohistochemistry. Fetal rat lungs were harvested at 13.5 dpc, cultured during 4 days and treated with increasing doses of ephrins-B1 and -B2 and the activity of key signaling pathways was assessed. Results: Ephrin-B1 presents mesenchymal expression, whereas ephrin-B2 and its receptor EphB4 were expressed by the epithelium. Both ephrins stimulated pulmonary branching. Moreover, while ephrin-B1 did not affect the pathways studied, ephrin-B2 supplementation decreased activity of JNK, ERK and STAT. This study characterizes the expression pattern of ephrins-B1, -B2 and EphB4 receptor throughout rat lung development. Conclusion: Our data highlight a possible role of ephrins as molecular stimulators of lung morphogenesis. Moreover, it supports the idea that classical vascular factors might play a role as airway growth promoters.http://www.karger.com/Article/FullText/369679Ephrin-B1Ephrin-B2EphB4Fetal lung developmentBranching |
| spellingShingle | Francisca O. Peixoto Patrícia Pereira-Terra Rute S. Moura Emanuel Carvalho-Dias Jorge Correia-Pinto Cristina Nogueira-Silva The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development Cellular Physiology and Biochemistry Ephrin-B1 Ephrin-B2 EphB4 Fetal lung development Branching |
| title | The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development |
| title_full | The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development |
| title_fullStr | The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development |
| title_full_unstemmed | The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development |
| title_short | The Role of Ephrins-B1 and -B2 During Fetal Rat Lung Development |
| title_sort | role of ephrins b1 and b2 during fetal rat lung development |
| topic | Ephrin-B1 Ephrin-B2 EphB4 Fetal lung development Branching |
| url | http://www.karger.com/Article/FullText/369679 |
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