Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock
Abstract WeedLock is a broad-spectrum plant-based bioherbicide that is currently on the market as a ready-to-use formulation. In this study, we investigated the physiological and biochemical effects of WeedLock (672.75 L ha-1) on Ageratum conyzoides L., Eleusine indica (L.) Gaertn, Zea mays L., and...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24144-2 |
| _version_ | 1828297046816194560 |
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| author | Mahmudul Hasan Anis Syahirah Mokhtar Khairil Mahmud Zulkarami Berahim Adam Mustafa Rosli Hafizuddin Hamdan Mst. Motmainna Muhammad Saiful Ahmad-Hamdani |
| author_facet | Mahmudul Hasan Anis Syahirah Mokhtar Khairil Mahmud Zulkarami Berahim Adam Mustafa Rosli Hafizuddin Hamdan Mst. Motmainna Muhammad Saiful Ahmad-Hamdani |
| author_sort | Mahmudul Hasan |
| collection | DOAJ |
| description | Abstract WeedLock is a broad-spectrum plant-based bioherbicide that is currently on the market as a ready-to-use formulation. In this study, we investigated the physiological and biochemical effects of WeedLock (672.75 L ha-1) on Ageratum conyzoides L., Eleusine indica (L.) Gaertn, Zea mays L., and Amaranthus gangeticus L. at four different time points. WeedLock caused significant reductions in chlorophyll pigment content and disrupted photosynthetic processes in all test plants. The greatest inhibition in photosynthesis was recorded in A. conyzoides at 24 h post-treatment with a 74.88% inhibition. Plants treated with WeedLock showed increased malondialdehyde (MDA) and proline production, which is indicative of phytotoxic stress. Remarkably, MDA contents of all treated plants increased by more than 100% in comparison to untreated. The activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) was elevated following treatment with WeedLock. Significant increases were observed in the SOD activity of A. conyzoides ranging from 69.66 to 118.24% from 6 to 72 h post-treatment. Our findings confirm that WeedLock disrupts the normal physiological and biochemical processes in plants following exposure and that its mode of action is associated with ROS (reactive oxygen species) production, similar to that of PPO (protoporphyrinogen oxidase) inhibitors, although specific site-of-action of this novel bioherbicide warrants further investigation. |
| first_indexed | 2024-04-13T12:21:07Z |
| format | Article |
| id | doaj.art-fd11258953eb4fddb2eea7ae8fbf09e5 |
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| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T12:21:07Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fd11258953eb4fddb2eea7ae8fbf09e52022-12-22T02:47:14ZengNature PortfolioScientific Reports2045-23222022-11-0112111010.1038/s41598-022-24144-2Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLockMahmudul Hasan0Anis Syahirah Mokhtar1Khairil Mahmud2Zulkarami Berahim3Adam Mustafa Rosli4Hafizuddin Hamdan5Mst. Motmainna6Muhammad Saiful Ahmad-Hamdani7Department of Crop Science, Faculty of Agriculture, Universiti Putra MalaysiaDepartment of Plant Protection, Faculty of Agriculture, Universiti Putra MalaysiaDepartment of Crop Science, Faculty of Agriculture, Universiti Putra MalaysiaLaboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra MalaysiaEntoGenex Industries Sdn. Bhd.EntoGenex Industries Sdn. Bhd.Department of Crop Science, Faculty of Agriculture, Universiti Putra MalaysiaDepartment of Crop Science, Faculty of Agriculture, Universiti Putra MalaysiaAbstract WeedLock is a broad-spectrum plant-based bioherbicide that is currently on the market as a ready-to-use formulation. In this study, we investigated the physiological and biochemical effects of WeedLock (672.75 L ha-1) on Ageratum conyzoides L., Eleusine indica (L.) Gaertn, Zea mays L., and Amaranthus gangeticus L. at four different time points. WeedLock caused significant reductions in chlorophyll pigment content and disrupted photosynthetic processes in all test plants. The greatest inhibition in photosynthesis was recorded in A. conyzoides at 24 h post-treatment with a 74.88% inhibition. Plants treated with WeedLock showed increased malondialdehyde (MDA) and proline production, which is indicative of phytotoxic stress. Remarkably, MDA contents of all treated plants increased by more than 100% in comparison to untreated. The activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) was elevated following treatment with WeedLock. Significant increases were observed in the SOD activity of A. conyzoides ranging from 69.66 to 118.24% from 6 to 72 h post-treatment. Our findings confirm that WeedLock disrupts the normal physiological and biochemical processes in plants following exposure and that its mode of action is associated with ROS (reactive oxygen species) production, similar to that of PPO (protoporphyrinogen oxidase) inhibitors, although specific site-of-action of this novel bioherbicide warrants further investigation.https://doi.org/10.1038/s41598-022-24144-2 |
| spellingShingle | Mahmudul Hasan Anis Syahirah Mokhtar Khairil Mahmud Zulkarami Berahim Adam Mustafa Rosli Hafizuddin Hamdan Mst. Motmainna Muhammad Saiful Ahmad-Hamdani Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock Scientific Reports |
| title | Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock |
| title_full | Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock |
| title_fullStr | Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock |
| title_full_unstemmed | Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock |
| title_short | Physiological and biochemical responses of selected weed and crop species to the plant-based bioherbicide WeedLock |
| title_sort | physiological and biochemical responses of selected weed and crop species to the plant based bioherbicide weedlock |
| url | https://doi.org/10.1038/s41598-022-24144-2 |
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