Hepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in mice
Abstract Background Penthorum chinense Pursh (Penthoraceae, PCP), a well-known Miao ethnomedicine, has been traditionally used to treat several liver-related diseases, such as jaundice and viral hepatitis. The aims of the present study were to evaluate the probable properties of the aqueous extract...
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| Format: | Article |
| Language: | English |
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BMC
2017-10-01
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| Series: | Chinese Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s13020-017-0153-x |
| _version_ | 1819157829848662016 |
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| author | Meng Wang Xiao-Jiao Zhang Ruibing Feng Yun Jiang Da-Yong Zhang Chengwei He Peng Li Jian-Bo Wan |
| author_facet | Meng Wang Xiao-Jiao Zhang Ruibing Feng Yun Jiang Da-Yong Zhang Chengwei He Peng Li Jian-Bo Wan |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | Abstract Background Penthorum chinense Pursh (Penthoraceae, PCP), a well-known Miao ethnomedicine, has been traditionally used to treat several liver-related diseases, such as jaundice and viral hepatitis. The aims of the present study were to evaluate the probable properties of the aqueous extract of PCP on carbon tetrachloride (CCl4)—induced acute liver injury in mice. Methods C57BL/6 mice were orally administered an aqueous extract of PCP (5.15 and 10.3 g/kg BW) or silymarin (100 mg/kg) once daily for 1 week prior to CCl4 exposure. Silymarin serves as a positive drug to validate the effectivenes of PCP. Results A single dose of CCl4 exposure caused severe acute liver injury in mice, as evidenced by the elevated serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alanine phosphatase (ALP), and the increased TUNEL-positive cells in liver, which were remarkably ameliorated by the pretreatment of PCP. PCP was also found to decrease the levels of malondialdehyde (MDA), restore the glutathione (GSH) and enhance the activities of superoxide dismutase (SOD) and catalase (CAT) in the liver. In addition, the pretreatment of PCP inhibited the degradation of hepatic cytochrome P450 2E1 (CYP2E1), up-regulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its target proteins in CCl4-treated mice. Conclusion Results indicated that the pretreatment of PCP (10.3 g/kg BW) effectively protected against CCl4-induced acute liver injury, which was comparable to efficacy of silymarin (100 mg/kg). This hepatoprotective effects might be attributed to amelioration of CCl4-induced oxidative stress via activating Nrf2 signaling pathway. |
| first_indexed | 2024-12-22T16:14:59Z |
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| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-22T16:14:59Z |
| publishDate | 2017-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-fd19a78f8261423799e56ff1a13ebecc2022-12-21T18:20:23ZengBMCChinese Medicine1749-85462017-10-0112111110.1186/s13020-017-0153-xHepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in miceMeng Wang0Xiao-Jiao Zhang1Ruibing Feng2Yun Jiang3Da-Yong Zhang4Chengwei He5Peng Li6Jian-Bo Wan7State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauSichuan New Lotus Traditional Chinese Herb Limited CompanySichuan New Lotus Traditional Chinese Herb Limited CompanyState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of MacauAbstract Background Penthorum chinense Pursh (Penthoraceae, PCP), a well-known Miao ethnomedicine, has been traditionally used to treat several liver-related diseases, such as jaundice and viral hepatitis. The aims of the present study were to evaluate the probable properties of the aqueous extract of PCP on carbon tetrachloride (CCl4)—induced acute liver injury in mice. Methods C57BL/6 mice were orally administered an aqueous extract of PCP (5.15 and 10.3 g/kg BW) or silymarin (100 mg/kg) once daily for 1 week prior to CCl4 exposure. Silymarin serves as a positive drug to validate the effectivenes of PCP. Results A single dose of CCl4 exposure caused severe acute liver injury in mice, as evidenced by the elevated serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alanine phosphatase (ALP), and the increased TUNEL-positive cells in liver, which were remarkably ameliorated by the pretreatment of PCP. PCP was also found to decrease the levels of malondialdehyde (MDA), restore the glutathione (GSH) and enhance the activities of superoxide dismutase (SOD) and catalase (CAT) in the liver. In addition, the pretreatment of PCP inhibited the degradation of hepatic cytochrome P450 2E1 (CYP2E1), up-regulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its target proteins in CCl4-treated mice. Conclusion Results indicated that the pretreatment of PCP (10.3 g/kg BW) effectively protected against CCl4-induced acute liver injury, which was comparable to efficacy of silymarin (100 mg/kg). This hepatoprotective effects might be attributed to amelioration of CCl4-induced oxidative stress via activating Nrf2 signaling pathway.http://link.springer.com/article/10.1186/s13020-017-0153-xCarbon tetrachlorideNuclear factor E2-related factor 2HepatotoxicityOxidative stressPenthorum chinense Pursh |
| spellingShingle | Meng Wang Xiao-Jiao Zhang Ruibing Feng Yun Jiang Da-Yong Zhang Chengwei He Peng Li Jian-Bo Wan Hepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in mice Chinese Medicine Carbon tetrachloride Nuclear factor E2-related factor 2 Hepatotoxicity Oxidative stress Penthorum chinense Pursh |
| title | Hepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in mice |
| title_full | Hepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in mice |
| title_fullStr | Hepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in mice |
| title_full_unstemmed | Hepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in mice |
| title_short | Hepatoprotective properties of Penthorum chinense Pursh against carbon tetrachloride-induced acute liver injury in mice |
| title_sort | hepatoprotective properties of penthorum chinense pursh against carbon tetrachloride induced acute liver injury in mice |
| topic | Carbon tetrachloride Nuclear factor E2-related factor 2 Hepatotoxicity Oxidative stress Penthorum chinense Pursh |
| url | http://link.springer.com/article/10.1186/s13020-017-0153-x |
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