Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver disease
Abstract Metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as nonalcoholic fatty liver disease (NAFLD), is associated with altered gut microbiota; however, there has been a focus on fecal samples, which are not representative of the entire digestive tract. Mucosal biop...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-59605-3 |
| _version_ | 1797199581728997376 |
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| author | Mengting Ren Hanghai Pan Xinxin Zhou Mosang Yu Feng Ji |
| author_facet | Mengting Ren Hanghai Pan Xinxin Zhou Mosang Yu Feng Ji |
| author_sort | Mengting Ren |
| collection | DOAJ |
| description | Abstract Metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as nonalcoholic fatty liver disease (NAFLD), is associated with altered gut microbiota; however, there has been a focus on fecal samples, which are not representative of the entire digestive tract. Mucosal biopsies of the descending duodenum were collected. Five regions of the 16S rRNA gene were amplified and sequenced. Other assessments conducted on the study subjects included body mass index, transient elastography, liver enzymes, and lipid profile. Fifty-one subjects (36 with MASLD and 15 controls) were evaluated. There was no significant difference between the two groups regarding alpha- or beta-diversity of the duodenal mucosal microbiota. Linear discriminant analysis effect size (LEfSe) analysis showed that the genera Serratia and Aggregatibacter were more abundant in the duodenal mucosa of patients with MASLD, whereas the duodenal mucosal microbiota of the healthy controls was enriched with the genus Petrobacter. PICRUSt2 analysis revealed that genes associated with amino acid degradation and carboxylate degradation were significantly enriched in the duodenal mucosal microbiota of patients with MASLD. Our findings reveal the duodenal mucosal microbiota in patients with MASLD, which could contribute to future studies investigating the causal relationship between duodenal microbiota and MASLD. |
| first_indexed | 2024-04-24T07:18:02Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T07:18:02Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fd1b96baf37e40b98aabf7c2e0d11c3d2024-04-21T11:14:19ZengNature PortfolioScientific Reports2045-23222024-04-011411910.1038/s41598-024-59605-3Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver diseaseMengting Ren0Hanghai Pan1Xinxin Zhou2Mosang Yu3Feng Ji4Department of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of MedicineDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of MedicineDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of MedicineDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of MedicineDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of MedicineAbstract Metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as nonalcoholic fatty liver disease (NAFLD), is associated with altered gut microbiota; however, there has been a focus on fecal samples, which are not representative of the entire digestive tract. Mucosal biopsies of the descending duodenum were collected. Five regions of the 16S rRNA gene were amplified and sequenced. Other assessments conducted on the study subjects included body mass index, transient elastography, liver enzymes, and lipid profile. Fifty-one subjects (36 with MASLD and 15 controls) were evaluated. There was no significant difference between the two groups regarding alpha- or beta-diversity of the duodenal mucosal microbiota. Linear discriminant analysis effect size (LEfSe) analysis showed that the genera Serratia and Aggregatibacter were more abundant in the duodenal mucosa of patients with MASLD, whereas the duodenal mucosal microbiota of the healthy controls was enriched with the genus Petrobacter. PICRUSt2 analysis revealed that genes associated with amino acid degradation and carboxylate degradation were significantly enriched in the duodenal mucosal microbiota of patients with MASLD. Our findings reveal the duodenal mucosal microbiota in patients with MASLD, which could contribute to future studies investigating the causal relationship between duodenal microbiota and MASLD.https://doi.org/10.1038/s41598-024-59605-3Gut microbiotaMetabolic dysfunction-associated steatotic liver diseaseSmall intestineMucosa-associated microbiota |
| spellingShingle | Mengting Ren Hanghai Pan Xinxin Zhou Mosang Yu Feng Ji Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver disease Scientific Reports Gut microbiota Metabolic dysfunction-associated steatotic liver disease Small intestine Mucosa-associated microbiota |
| title | Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver disease |
| title_full | Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver disease |
| title_fullStr | Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver disease |
| title_full_unstemmed | Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver disease |
| title_short | Alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction-associated steatotic liver disease |
| title_sort | alterations of the duodenal mucosal microbiome in patients with metabolic dysfunction associated steatotic liver disease |
| topic | Gut microbiota Metabolic dysfunction-associated steatotic liver disease Small intestine Mucosa-associated microbiota |
| url | https://doi.org/10.1038/s41598-024-59605-3 |
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