Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5p
Extracellular vesicles are involved in many physiological and pathological activities. They transport miRNAs to recipient cells during their role in intercellular communication, making them emerging biomarkers of many diseases. Interest in exosomal miRNAs has grown after they have shown numerous adv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/5/189 |
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| author | Verónica Morgado Serrano Inês Simões Patrício Silva Ana Rita Cardoso Maria Goreti Ferreira Sales |
| author_facet | Verónica Morgado Serrano Inês Simões Patrício Silva Ana Rita Cardoso Maria Goreti Ferreira Sales |
| author_sort | Verónica Morgado Serrano |
| collection | DOAJ |
| description | Extracellular vesicles are involved in many physiological and pathological activities. They transport miRNAs to recipient cells during their role in intercellular communication, making them emerging biomarkers of many diseases. Interest in exosomal miRNAs has grown after they have shown numerous advantages as biomarkers for diagnosis, prognosis, and evaluation of cancer treatment. This work describes the development of a biosensor for the detection of 21-5p miRNA in human serum using screen-printed carbon electrodes modified with gold nanoparticles fabricated in situ, an innovative approach to avoid the use of more expensive gold substrates that provide better analytical outputs. The several variables involved in the assembly of the biosensor were optimized by univariant mode. Under the best conditions, the biosensor showed a linear response from 0.010 fM to 10 pM, with a limit of detection (LOD) of 4.31 aM. The sensitivity was 0.3718 relative Ω per decade concentration in buffered saline solutions, and the standard deviation of the blank is 2.94 Ω. A linear response was also obtained when human serum samples were tested with miRNA 21-5p. Interference from similar miRNA and miss-match miRNA sequences was evaluated and good selectivity for miRNA 21-5p was observed. Overall, the device proposed is an alternative approach to gold substrates, which typically result in more sensitive systems and lower LODs, which compares favorably to current gold-based biosensors for the targeted miRNA. This design may be further extended to other nucleic acids. |
| first_indexed | 2024-03-10T03:10:13Z |
| format | Article |
| id | doaj.art-fd1c7a982d5149f692f80554df705652 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T03:10:13Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-fd1c7a982d5149f692f80554df7056522023-11-23T10:29:16ZengMDPI AGChemosensors2227-90402022-05-0110518910.3390/chemosensors10050189Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5pVerónica Morgado Serrano0Inês Simões Patrício Silva1Ana Rita Cardoso2Maria Goreti Ferreira Sales3BioMark@UC/CEB-LABBELS, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, PortugalBioMark@UC/CEB-LABBELS, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, PortugalBioMark@UC/CEB-LABBELS, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, PortugalBioMark@UC/CEB-LABBELS, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, PortugalExtracellular vesicles are involved in many physiological and pathological activities. They transport miRNAs to recipient cells during their role in intercellular communication, making them emerging biomarkers of many diseases. Interest in exosomal miRNAs has grown after they have shown numerous advantages as biomarkers for diagnosis, prognosis, and evaluation of cancer treatment. This work describes the development of a biosensor for the detection of 21-5p miRNA in human serum using screen-printed carbon electrodes modified with gold nanoparticles fabricated in situ, an innovative approach to avoid the use of more expensive gold substrates that provide better analytical outputs. The several variables involved in the assembly of the biosensor were optimized by univariant mode. Under the best conditions, the biosensor showed a linear response from 0.010 fM to 10 pM, with a limit of detection (LOD) of 4.31 aM. The sensitivity was 0.3718 relative Ω per decade concentration in buffered saline solutions, and the standard deviation of the blank is 2.94 Ω. A linear response was also obtained when human serum samples were tested with miRNA 21-5p. Interference from similar miRNA and miss-match miRNA sequences was evaluated and good selectivity for miRNA 21-5p was observed. Overall, the device proposed is an alternative approach to gold substrates, which typically result in more sensitive systems and lower LODs, which compares favorably to current gold-based biosensors for the targeted miRNA. This design may be further extended to other nucleic acids.https://www.mdpi.com/2227-9040/10/5/189extracellular vesiclesnucleic acidsmiRNA 21-5pelectrochemical biosensorcancer biomarkersneurological biomarkers |
| spellingShingle | Verónica Morgado Serrano Inês Simões Patrício Silva Ana Rita Cardoso Maria Goreti Ferreira Sales Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5p Chemosensors extracellular vesicles nucleic acids miRNA 21-5p electrochemical biosensor cancer biomarkers neurological biomarkers |
| title | Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5p |
| title_full | Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5p |
| title_fullStr | Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5p |
| title_full_unstemmed | Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5p |
| title_short | Carbon Electrodes with Gold Nanoparticles for the Electrochemical Detection of miRNA 21-5p |
| title_sort | carbon electrodes with gold nanoparticles for the electrochemical detection of mirna 21 5p |
| topic | extracellular vesicles nucleic acids miRNA 21-5p electrochemical biosensor cancer biomarkers neurological biomarkers |
| url | https://www.mdpi.com/2227-9040/10/5/189 |
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