An investigative study on the zoonotic potential of Helicobacter pylori
Abstract Background Helicobacter pylori is one of the most common bacterial infections and is widespread globally. It causes a variety of gastrointestinal disorders, though a great proportion of infections are asymptomatic. A total of 143 fresh stool samples were collected from apparently healthy fa...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-023-03572-w |
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| author | Sabah I. Shaaban Dalia Talat Shymaa A. Khatab Mohamed A. Nossair Mousa A. Ayoub Rania M. Ewida Mohamed Said Diab |
| author_facet | Sabah I. Shaaban Dalia Talat Shymaa A. Khatab Mohamed A. Nossair Mousa A. Ayoub Rania M. Ewida Mohamed Said Diab |
| author_sort | Sabah I. Shaaban |
| collection | DOAJ |
| description | Abstract Background Helicobacter pylori is one of the most common bacterial infections and is widespread globally. It causes a variety of gastrointestinal disorders, though a great proportion of infections are asymptomatic. A total of 143 fresh stool samples were collected from apparently healthy farm and pet animals (43 cattle, 50 buffaloes, 50 sheep, 50 dogs, and 50 cats), in addition to 768 human stool samples. The samples were examined using stool antigen and rapid antibody tests, and further confirmation of glmM “human antigen-positive samples and animal milk samples” was conducted by polymerase chain reaction (PCR). Results The prevalence rates of H. pylori infection in animals were 22.2% and 16% in antibody and stool antigen tests, respectively. The detection rates were 28%, 24%, 12%, 10%, and 4.7% in cats, dogs, buffaloes, sheep, and cattle, respectively. On the other hand, the prevalence rate of H. pylori infection in human stool samples was 74.8%, and a statistically significant association was observed between prevalence and several factors, such as sex, age, and locality. PCR was performed to detect the glmM gene of H. pylori, and this gene was found in 21 of 27 human antigen-positive samples and 5 of 13 animal milk samples. Conclusions H. pylori was detected in both human and animal samples. Furthermore, glmM was found in milk and human samples. Our findings suggest that pet and farm animals could transmit H. pylori infection to humans. |
| first_indexed | 2024-04-10T21:02:43Z |
| format | Article |
| id | doaj.art-fd1cb86d51f04d9094ccf204a1d6356a |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-10T21:02:43Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-fd1cb86d51f04d9094ccf204a1d6356a2023-01-22T12:16:47ZengBMCBMC Veterinary Research1746-61482023-01-011911710.1186/s12917-023-03572-wAn investigative study on the zoonotic potential of Helicobacter pyloriSabah I. Shaaban0Dalia Talat1Shymaa A. Khatab2Mohamed A. Nossair3Mousa A. Ayoub4Rania M. Ewida5Mohamed Said Diab6Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Damanhour UniversityDepartment of Microbiology, Faculty of Veterinary Medicine, Damanhour UniversityGenetics and Genetic Engineering. Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria UniversityDepartment of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Alexandria UniversityDepartment of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Damanhour UniversityDepartment of Food Hygiene (Milk Hygiene), Faculty of Veterinary Medicine, New Valley UniversityDepartment of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, New Valley UniversityAbstract Background Helicobacter pylori is one of the most common bacterial infections and is widespread globally. It causes a variety of gastrointestinal disorders, though a great proportion of infections are asymptomatic. A total of 143 fresh stool samples were collected from apparently healthy farm and pet animals (43 cattle, 50 buffaloes, 50 sheep, 50 dogs, and 50 cats), in addition to 768 human stool samples. The samples were examined using stool antigen and rapid antibody tests, and further confirmation of glmM “human antigen-positive samples and animal milk samples” was conducted by polymerase chain reaction (PCR). Results The prevalence rates of H. pylori infection in animals were 22.2% and 16% in antibody and stool antigen tests, respectively. The detection rates were 28%, 24%, 12%, 10%, and 4.7% in cats, dogs, buffaloes, sheep, and cattle, respectively. On the other hand, the prevalence rate of H. pylori infection in human stool samples was 74.8%, and a statistically significant association was observed between prevalence and several factors, such as sex, age, and locality. PCR was performed to detect the glmM gene of H. pylori, and this gene was found in 21 of 27 human antigen-positive samples and 5 of 13 animal milk samples. Conclusions H. pylori was detected in both human and animal samples. Furthermore, glmM was found in milk and human samples. Our findings suggest that pet and farm animals could transmit H. pylori infection to humans.https://doi.org/10.1186/s12917-023-03572-wAnimalglmM geneH. pyloriHumanMilkZoonosis |
| spellingShingle | Sabah I. Shaaban Dalia Talat Shymaa A. Khatab Mohamed A. Nossair Mousa A. Ayoub Rania M. Ewida Mohamed Said Diab An investigative study on the zoonotic potential of Helicobacter pylori BMC Veterinary Research Animal glmM gene H. pylori Human Milk Zoonosis |
| title | An investigative study on the zoonotic potential of Helicobacter pylori |
| title_full | An investigative study on the zoonotic potential of Helicobacter pylori |
| title_fullStr | An investigative study on the zoonotic potential of Helicobacter pylori |
| title_full_unstemmed | An investigative study on the zoonotic potential of Helicobacter pylori |
| title_short | An investigative study on the zoonotic potential of Helicobacter pylori |
| title_sort | investigative study on the zoonotic potential of helicobacter pylori |
| topic | Animal glmM gene H. pylori Human Milk Zoonosis |
| url | https://doi.org/10.1186/s12917-023-03572-w |
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