High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC
Cells under cyclic stretch sense their environments and induce responses such as actin stress fiber (SF) reorientation and morphological changes. These physiological responses are thought to occur when cells sense incompatibility between SF orientation and stretching direction. This hypothesis requi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The Japan Society of Mechanical Engineers
2012-03-01
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| Series: | Journal of Biomechanical Science and Engineering |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jbse/7/2/7_2_188/_pdf/-char/en |
| _version_ | 1818521655934189568 |
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| author | Yusuke MITSUOKA Akira TSUKAMOTO Shunsuke IWAYOSHI Katsuko S. FURUKAWA Takashi USHIDA |
| author_facet | Yusuke MITSUOKA Akira TSUKAMOTO Shunsuke IWAYOSHI Katsuko S. FURUKAWA Takashi USHIDA |
| author_sort | Yusuke MITSUOKA |
| collection | DOAJ |
| description | Cells under cyclic stretch sense their environments and induce responses such as actin stress fiber (SF) reorientation and morphological changes. These physiological responses are thought to occur when cells sense incompatibility between SF orientation and stretching direction. This hypothesis requires existence of SFs. However, such existence of SFs in cells under cyclic stretch remains unclear since few studies attempted to track the existence of SFs throughout cyclic stretch. In order to track the existence of SFs throughout cyclic stretch, high time resolution time-lapse imaging was improved in two points. First, SFs were clearly imaged with coexpression of DsRed-zyxin and GFP-actin. Second, time resolution was improved so that fluorescence images were obtained every 28 sec. With the improved high time resolution time-lapse imaging, it was revealed, for the first time, that SFs could exist continuously throughout cyclic stretch. Moreover, physiological responses including morphological change as well as SF reorientation occurred during the time when SFs formed incompatibility between SF orientation and stretching direction. These results demonstrated that SFs continuously existed in cells under cyclic stretch and in turn suggested that continuous presence of incompatibility between orientation of long-lasting SFs and the stretching direction might be important for mechanosensing which induces physiological responses. |
| first_indexed | 2024-12-11T01:54:15Z |
| format | Article |
| id | doaj.art-fd1f9f20ba8345ac883522ef3ae3879c |
| institution | Directory Open Access Journal |
| issn | 1880-9863 |
| language | English |
| last_indexed | 2024-12-11T01:54:15Z |
| publishDate | 2012-03-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Journal of Biomechanical Science and Engineering |
| spelling | doaj.art-fd1f9f20ba8345ac883522ef3ae3879c2022-12-22T01:24:40ZengThe Japan Society of Mechanical EngineersJournal of Biomechanical Science and Engineering1880-98632012-03-017218819810.1299/jbse.7.188jbseHigh Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVECYusuke MITSUOKA0Akira TSUKAMOTO1Shunsuke IWAYOSHI2Katsuko S. FURUKAWA3Takashi USHIDA4Department of Mechanical Engineering, Graduate School of Engineering, The University of TokyoDepatment of Applied Physics, National Defense AcademyDepartment of Mechanical Engineering, Graduate School of Engineering, The University of TokyoDepartment of Bioengineering, Graduate School of Engineering, The University of TokyoDepartment of Mechanical Engineering, Graduate School of Engineering, The University of TokyoCells under cyclic stretch sense their environments and induce responses such as actin stress fiber (SF) reorientation and morphological changes. These physiological responses are thought to occur when cells sense incompatibility between SF orientation and stretching direction. This hypothesis requires existence of SFs. However, such existence of SFs in cells under cyclic stretch remains unclear since few studies attempted to track the existence of SFs throughout cyclic stretch. In order to track the existence of SFs throughout cyclic stretch, high time resolution time-lapse imaging was improved in two points. First, SFs were clearly imaged with coexpression of DsRed-zyxin and GFP-actin. Second, time resolution was improved so that fluorescence images were obtained every 28 sec. With the improved high time resolution time-lapse imaging, it was revealed, for the first time, that SFs could exist continuously throughout cyclic stretch. Moreover, physiological responses including morphological change as well as SF reorientation occurred during the time when SFs formed incompatibility between SF orientation and stretching direction. These results demonstrated that SFs continuously existed in cells under cyclic stretch and in turn suggested that continuous presence of incompatibility between orientation of long-lasting SFs and the stretching direction might be important for mechanosensing which induces physiological responses.https://www.jstage.jst.go.jp/article/jbse/7/2/7_2_188/_pdf/-char/encyclic stretchstress fiberorientation dynamicsmorphological changetime-lapse imaginghuvec |
| spellingShingle | Yusuke MITSUOKA Akira TSUKAMOTO Shunsuke IWAYOSHI Katsuko S. FURUKAWA Takashi USHIDA High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC Journal of Biomechanical Science and Engineering cyclic stretch stress fiber orientation dynamics morphological change time-lapse imaging huvec |
| title | High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC |
| title_full | High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC |
| title_fullStr | High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC |
| title_full_unstemmed | High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC |
| title_short | High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC |
| title_sort | high time resolution time lapse imaging reveals continuous existence and rotation of stress fibers under cyclic stretch in huvec |
| topic | cyclic stretch stress fiber orientation dynamics morphological change time-lapse imaging huvec |
| url | https://www.jstage.jst.go.jp/article/jbse/7/2/7_2_188/_pdf/-char/en |
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