Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation
Abstract Composites of polymer and graphene-based nanomaterials (GBNs) combine easy processing onto porous 3D membrane geometries due to the polymer and cellular differentiation stimuli due to GBNs fillers. Aiming to step forward to the clinical application of polymer/GBNs composites, this study per...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-17697-9 |
| _version_ | 1811315441132371968 |
|---|---|
| author | Marián Mantecón-Oria Olga Tapia Miguel Lafarga María T. Berciano Jose M. Munuera Silvia Villar-Rodil Juan I. Paredes María J. Rivero Nazely Diban Ane Urtiaga |
| author_facet | Marián Mantecón-Oria Olga Tapia Miguel Lafarga María T. Berciano Jose M. Munuera Silvia Villar-Rodil Juan I. Paredes María J. Rivero Nazely Diban Ane Urtiaga |
| author_sort | Marián Mantecón-Oria |
| collection | DOAJ |
| description | Abstract Composites of polymer and graphene-based nanomaterials (GBNs) combine easy processing onto porous 3D membrane geometries due to the polymer and cellular differentiation stimuli due to GBNs fillers. Aiming to step forward to the clinical application of polymer/GBNs composites, this study performs a systematic and detailed comparative analysis of the influence of the properties of four different GBNs: (i) graphene oxide obtained from graphite chemically processes (GO); (ii) reduced graphene oxide (rGO); (iii) multilayered graphene produced by mechanical exfoliation method (Gmec); and (iv) low-oxidized graphene via anodic exfoliation (Ganodic); dispersed in polycaprolactone (PCL) porous membranes to induce astrocytic differentiation. PCL/GBN flat membranes were fabricated by phase inversion technique and broadly characterized in morphology and topography, chemical structure, hydrophilicity, protein adsorption, and electrical properties. Cellular assays with rat C6 glioma cells, as model for cell-specific astrocytes, were performed. Remarkably, low GBN loading (0.67 wt%) caused an important difference in the response of the C6 differentiation among PCL/GBN membranes. PCL/rGO and PCL/GO membranes presented the highest biomolecule markers for astrocyte differentiation. Our results pointed to the chemical structural defects in rGO and GO nanomaterials and the protein adsorption mechanisms as the most plausible cause conferring distinctive properties to PCL/GBN membranes for the promotion of astrocytic differentiation. Overall, our systematic comparative study provides generalizable conclusions and new evidences to discern the role of GBNs features for future research on 3D PCL/graphene composite hollow fiber membranes for in vitro neural models. |
| first_indexed | 2024-04-13T11:30:20Z |
| format | Article |
| id | doaj.art-fd2af4957ef64a2097c573b8b9ebf7c8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T11:30:20Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fd2af4957ef64a2097c573b8b9ebf7c82022-12-22T02:48:35ZengNature PortfolioScientific Reports2045-23222022-08-0112111510.1038/s41598-022-17697-9Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiationMarián Mantecón-Oria0Olga Tapia1Miguel Lafarga2María T. Berciano3Jose M. Munuera4Silvia Villar-Rodil5Juan I. Paredes6María J. Rivero7Nazely Diban8Ane Urtiaga9Departamento de Ingenierias Química y Biomolecular, Universidad de CantabriaResearch Group on Food, Nutritional Biochemistry and Health, Universidad Europea del AtlánticoInstituto Marqués de Valdecilla (IDIVAL)Instituto Marqués de Valdecilla (IDIVAL)Instituto de Ciencia y Tecnología del Carbono, INCAR-CSICInstituto de Ciencia y Tecnología del Carbono, INCAR-CSICInstituto de Ciencia y Tecnología del Carbono, INCAR-CSICDepartamento de Ingenierias Química y Biomolecular, Universidad de CantabriaDepartamento de Ingenierias Química y Biomolecular, Universidad de CantabriaDepartamento de Ingenierias Química y Biomolecular, Universidad de CantabriaAbstract Composites of polymer and graphene-based nanomaterials (GBNs) combine easy processing onto porous 3D membrane geometries due to the polymer and cellular differentiation stimuli due to GBNs fillers. Aiming to step forward to the clinical application of polymer/GBNs composites, this study performs a systematic and detailed comparative analysis of the influence of the properties of four different GBNs: (i) graphene oxide obtained from graphite chemically processes (GO); (ii) reduced graphene oxide (rGO); (iii) multilayered graphene produced by mechanical exfoliation method (Gmec); and (iv) low-oxidized graphene via anodic exfoliation (Ganodic); dispersed in polycaprolactone (PCL) porous membranes to induce astrocytic differentiation. PCL/GBN flat membranes were fabricated by phase inversion technique and broadly characterized in morphology and topography, chemical structure, hydrophilicity, protein adsorption, and electrical properties. Cellular assays with rat C6 glioma cells, as model for cell-specific astrocytes, were performed. Remarkably, low GBN loading (0.67 wt%) caused an important difference in the response of the C6 differentiation among PCL/GBN membranes. PCL/rGO and PCL/GO membranes presented the highest biomolecule markers for astrocyte differentiation. Our results pointed to the chemical structural defects in rGO and GO nanomaterials and the protein adsorption mechanisms as the most plausible cause conferring distinctive properties to PCL/GBN membranes for the promotion of astrocytic differentiation. Overall, our systematic comparative study provides generalizable conclusions and new evidences to discern the role of GBNs features for future research on 3D PCL/graphene composite hollow fiber membranes for in vitro neural models.https://doi.org/10.1038/s41598-022-17697-9 |
| spellingShingle | Marián Mantecón-Oria Olga Tapia Miguel Lafarga María T. Berciano Jose M. Munuera Silvia Villar-Rodil Juan I. Paredes María J. Rivero Nazely Diban Ane Urtiaga Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation Scientific Reports |
| title | Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation |
| title_full | Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation |
| title_fullStr | Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation |
| title_full_unstemmed | Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation |
| title_short | Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation |
| title_sort | influence of the properties of different graphene based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation |
| url | https://doi.org/10.1038/s41598-022-17697-9 |
| work_keys_str_mv | AT marianmanteconoria influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT olgatapia influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT miguellafarga influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT mariatberciano influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT josemmunuera influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT silviavillarrodil influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT juaniparedes influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT mariajrivero influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT nazelydiban influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation AT aneurtiaga influenceofthepropertiesofdifferentgraphenebasednanomaterialsdispersedinpolycaprolactonemembranesonastrocyticdifferentiation |