The Matrix Effect in the RT-PCR Detection of SARS-CoV-2 Using Saliva without RNA Extraction

The Matrix Effect in the RT-PCR Detection of SARS-CoV-2 Using Saliva without RNA Extraction

The present work focuses on the detection of SARS-CoV-2 in saliva, contributing to understanding the inhibition effect of the matrix and its influence on the results. Detection of viral genes ORF1ab, N, and E was performed by RT-PCR using saliva directly in the reaction without RNA extraction. Diffe...

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Bibliographic Details
Main Authors: Orlando Morais, Manuel Rui Alves, Carla Ramos, Fernando Ferreira, Paulo Fernandes
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Diagnostics
Subjects:
SARS-CoV-2
saliva
RT-PCR
Ct values
sensitivity
detection
Online Access:https://www.mdpi.com/2075-4418/12/7/1547

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