Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joining
Using X-ray crystallography and nonhomologous end-joining assays, this study reveals structural features within Polλ that provide it with the ability to bridge and stabilize tenuous DNA double-strand break ends, allowing for religation.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-31278-4 |
| _version_ | 1818234324167688192 |
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| author | Andrea M. Kaminski Kishore K. Chiruvella Dale A. Ramsden Katarzyna Bebenek Thomas A. Kunkel Lars C. Pedersen |
| author_facet | Andrea M. Kaminski Kishore K. Chiruvella Dale A. Ramsden Katarzyna Bebenek Thomas A. Kunkel Lars C. Pedersen |
| author_sort | Andrea M. Kaminski |
| collection | DOAJ |
| description | Using X-ray crystallography and nonhomologous end-joining assays, this study reveals structural features within Polλ that provide it with the ability to bridge and stabilize tenuous DNA double-strand break ends, allowing for religation. |
| first_indexed | 2024-12-12T11:36:16Z |
| format | Article |
| id | doaj.art-fd3014798ddc4531986c69cc86695666 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-12T11:36:16Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-fd3014798ddc4531986c69cc866956662022-12-22T00:25:40ZengNature PortfolioNature Communications2041-17232022-07-0113111410.1038/s41467-022-31278-4Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joiningAndrea M. Kaminski0Kishore K. Chiruvella1Dale A. Ramsden2Katarzyna Bebenek3Thomas A. Kunkel4Lars C. Pedersen5Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthDepartment of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel HillDepartment of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel HillGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of HealthUsing X-ray crystallography and nonhomologous end-joining assays, this study reveals structural features within Polλ that provide it with the ability to bridge and stabilize tenuous DNA double-strand break ends, allowing for religation.https://doi.org/10.1038/s41467-022-31278-4 |
| spellingShingle | Andrea M. Kaminski Kishore K. Chiruvella Dale A. Ramsden Katarzyna Bebenek Thomas A. Kunkel Lars C. Pedersen Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joining Nature Communications |
| title | Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joining |
| title_full | Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joining |
| title_fullStr | Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joining |
| title_full_unstemmed | Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joining |
| title_short | Analysis of diverse double-strand break synapsis with Polλ reveals basis for unique substrate specificity in nonhomologous end-joining |
| title_sort | analysis of diverse double strand break synapsis with polλ reveals basis for unique substrate specificity in nonhomologous end joining |
| url | https://doi.org/10.1038/s41467-022-31278-4 |
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