The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp
Fipronil (FPN) is an insecticide used in agriculture. This study focused on the biotransformation process and the histopathological effects of FPN in the liver of grass carp. Fish were exposed to environmental concentrations of FPN (3, 6, and 10 ug l-1) for up to 14 d. The alterations in phase I and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Inter-Research
2024-03-01
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| Series: | Aquatic Biology |
| Online Access: | https://www.int-res.com/abstracts/ab/v33/p47-53/ |
| _version_ | 1797214527864963072 |
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| author | R Alijani Ardeshir S Shahidokht M Mirzaei S Rastgar |
| author_facet | R Alijani Ardeshir S Shahidokht M Mirzaei S Rastgar |
| author_sort | R Alijani Ardeshir |
| collection | DOAJ |
| description | Fipronil (FPN) is an insecticide used in agriculture. This study focused on the biotransformation process and the histopathological effects of FPN in the liver of grass carp. Fish were exposed to environmental concentrations of FPN (3, 6, and 10 ug l-1) for up to 14 d. The alterations in phase I and II biotransformation enzyme activity (ethoxyresorufin-O-deethylase [EROD] and glutathione-S-transferase [GST]), malondialdehyde (MDA) content, and histopathology in the liver were studied on the 1st, 3rd, 7th, and 14th days. Results showed that EROD (dose-dependent) and GST activity (time-dependent) increased. The MDA content increased in a time- and dose-dependent manner. The most common types of hepatological damage were steatosis, vein dilatation, pyknosis, and increased melanomacrophage centers, probably due to oxidative stress originating from biotransformation enzyme activity (R2 = 0.88 for GST and MDA). The degree of tissue change (DTC) at the highest dose indicated moderate damage to the liver (R2 = 0.82 for GST and DTC). Nevertheless, the level of EROD and GST activity and MDA content indicated complex interactions among various phase I and phase II biotransformation enzymes which should be investigated in future studies with more replications. |
| first_indexed | 2024-04-24T11:15:36Z |
| format | Article |
| id | doaj.art-fd311cc6dcd3413c849f24fe1aad6a0f |
| institution | Directory Open Access Journal |
| issn | 1864-7782 1864-7790 |
| language | English |
| last_indexed | 2024-04-24T11:15:36Z |
| publishDate | 2024-03-01 |
| publisher | Inter-Research |
| record_format | Article |
| series | Aquatic Biology |
| spelling | doaj.art-fd311cc6dcd3413c849f24fe1aad6a0f2024-04-11T09:53:35ZengInter-ResearchAquatic Biology1864-77821864-77902024-03-0133475310.3354/ab00765The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carpR Alijani Ardeshir0S Shahidokht1M Mirzaei2S Rastgar3Marine Biotechnology Department, College of Biotechnology, Amol University of Special Modern Technologies, Amol 4615664616, IranIslamic Azad University of Sari, Faculty of Medical Science, Sari 48164194, IranIslamic Azad University of Sari, Faculty of Medical Science, Sari 48164194, IranDepartment of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, PO Box 669, Khorramshahr 6419934619, IranFipronil (FPN) is an insecticide used in agriculture. This study focused on the biotransformation process and the histopathological effects of FPN in the liver of grass carp. Fish were exposed to environmental concentrations of FPN (3, 6, and 10 ug l-1) for up to 14 d. The alterations in phase I and II biotransformation enzyme activity (ethoxyresorufin-O-deethylase [EROD] and glutathione-S-transferase [GST]), malondialdehyde (MDA) content, and histopathology in the liver were studied on the 1st, 3rd, 7th, and 14th days. Results showed that EROD (dose-dependent) and GST activity (time-dependent) increased. The MDA content increased in a time- and dose-dependent manner. The most common types of hepatological damage were steatosis, vein dilatation, pyknosis, and increased melanomacrophage centers, probably due to oxidative stress originating from biotransformation enzyme activity (R2 = 0.88 for GST and MDA). The degree of tissue change (DTC) at the highest dose indicated moderate damage to the liver (R2 = 0.82 for GST and DTC). Nevertheless, the level of EROD and GST activity and MDA content indicated complex interactions among various phase I and phase II biotransformation enzymes which should be investigated in future studies with more replications.https://www.int-res.com/abstracts/ab/v33/p47-53/ |
| spellingShingle | R Alijani Ardeshir S Shahidokht M Mirzaei S Rastgar The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp Aquatic Biology |
| title | The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp |
| title_full | The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp |
| title_fullStr | The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp |
| title_full_unstemmed | The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp |
| title_short | The effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp |
| title_sort | effect of fipronil exposure on the activity of biotransformation enzymes and histology in the liver of grass carp |
| url | https://www.int-res.com/abstracts/ab/v33/p47-53/ |
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