NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC)
The purpose of the study was to assess the prognostic significance of a scaffolding NEDD9 protein in patients with non-small cell lung cancer (NSCLC). NEDD9 (Neural precursor cell expressed, developmentally down-regulated 9) is a scaffolding protein in many intracellular protein cascades, such as PI...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Tomsk National Research Medical Center
2022-09-01
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| Series: | Сибирский онкологический журнал |
| Subjects: | |
| Online Access: | https://www.siboncoj.ru/jour/article/view/2242 |
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| author | M. V. Tikhomirova V. V. Barmin Yu. A. Topchu A. M. Mazitova E. Yu. Ratner A. G. Sabirov Z. I. Аbramova A. Ya. Deneka |
| author_facet | M. V. Tikhomirova V. V. Barmin Yu. A. Topchu A. M. Mazitova E. Yu. Ratner A. G. Sabirov Z. I. Аbramova A. Ya. Deneka |
| author_sort | M. V. Tikhomirova |
| collection | DOAJ |
| description | The purpose of the study was to assess the prognostic significance of a scaffolding NEDD9 protein in patients with non-small cell lung cancer (NSCLC). NEDD9 (Neural precursor cell expressed, developmentally down-regulated 9) is a scaffolding protein in many intracellular protein cascades, such as PI3K-AKT-mTOR, Ras-ERK, which play key roles in tumor progression. several studies define the regulatory role of NEDD9 in progression of several solid cancers, including non-small cell lung cancer (NSCLC), however its role as a prediction biomarker is minimally assessed. Materials and methods. In the current study quantitative immunohistochemical (IHC) approach was utilized to characterize NEDD9 expression in a cohort of NSCLC primary tumor samples (n=16), and correlative statistical analysis was performed between NEDD9 expression and a set of patients’ clinical and pathological characteristics. Results of the IHC analysis were validated using broader TCGA RNAseq dataset (n=566). Results. The study demonstrated significant difference between the expression of Nedd9 in stage iii tumors versus stages II and IV (p<0.05). Next, NEDD9 expression level was approximated to high and low (based on median h-score=56.14) and statistical survival analysis revealed a positive correlative trend between the decreased expression of NEDD9 and the decrease in the overall (OS) and progression-free survival (PFS) of the patients. Analysis of TCGA dataset harboring RNA seq data confirmed statistically significant correlation (p=0.05) between low NEDD9 expression and decreased OS. Conclusion. This study suggests that the expression of the scaffolding protein NEDD9, both at the protein and RNA levels, positively correlates with a negative prognosis in NSCLC, suggesting that it can potentially be used as a novel biomarker of disease progression. Further basic and clinical research defining the role of NEDD9 in the progression and metastasis of NSCLC are strongly warranted. |
| first_indexed | 2024-04-10T01:51:43Z |
| format | Article |
| id | doaj.art-fd33747a78dc48e99ba9ce0bf9ebdea9 |
| institution | Directory Open Access Journal |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| last_indexed | 2024-04-10T01:51:43Z |
| publishDate | 2022-09-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj.art-fd33747a78dc48e99ba9ce0bf9ebdea92023-03-13T09:05:56ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682022-09-01214475510.21294/1814-4861-2022-21-4-47-551026NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC)M. V. Tikhomirova0V. V. Barmin1Yu. A. Topchu2A. M. Mazitova3E. Yu. Ratner4A. G. Sabirov5Z. I. Аbramova6A. Ya. Deneka7Казанский (Приволжский) федеральный университетМосковский научно-исследовательский онкологический институт им. П.А. Герцена – филиал ФГБУ «Национальный медицинский исследовательский центр радиологии» Минздрава РоссииКазанский (Приволжский) федеральный университетКазанский (Приволжский) федеральный университетРеспубликанский клинический онкологический диспансер Минздрава Республики Татарстан им. проф. М.З. СигалаРеспубликанский клинический онкологический диспансер Минздрава Республики Татарстан им. проф. М.З. СигалаКазанский (Приволжский) федеральный университетКазанский (Приволжский) федеральный университетThe purpose of the study was to assess the prognostic significance of a scaffolding NEDD9 protein in patients with non-small cell lung cancer (NSCLC). NEDD9 (Neural precursor cell expressed, developmentally down-regulated 9) is a scaffolding protein in many intracellular protein cascades, such as PI3K-AKT-mTOR, Ras-ERK, which play key roles in tumor progression. several studies define the regulatory role of NEDD9 in progression of several solid cancers, including non-small cell lung cancer (NSCLC), however its role as a prediction biomarker is minimally assessed. Materials and methods. In the current study quantitative immunohistochemical (IHC) approach was utilized to characterize NEDD9 expression in a cohort of NSCLC primary tumor samples (n=16), and correlative statistical analysis was performed between NEDD9 expression and a set of patients’ clinical and pathological characteristics. Results of the IHC analysis were validated using broader TCGA RNAseq dataset (n=566). Results. The study demonstrated significant difference between the expression of Nedd9 in stage iii tumors versus stages II and IV (p<0.05). Next, NEDD9 expression level was approximated to high and low (based on median h-score=56.14) and statistical survival analysis revealed a positive correlative trend between the decreased expression of NEDD9 and the decrease in the overall (OS) and progression-free survival (PFS) of the patients. Analysis of TCGA dataset harboring RNA seq data confirmed statistically significant correlation (p=0.05) between low NEDD9 expression and decreased OS. Conclusion. This study suggests that the expression of the scaffolding protein NEDD9, both at the protein and RNA levels, positively correlates with a negative prognosis in NSCLC, suggesting that it can potentially be used as a novel biomarker of disease progression. Further basic and clinical research defining the role of NEDD9 in the progression and metastasis of NSCLC are strongly warranted.https://www.siboncoj.ru/jour/article/view/2242немелкоклеточный рак легкогообщая выживаемостьбеспрогрессивная выживаемостьподдерживающие белкиnedd9маркеры-предикторы |
| spellingShingle | M. V. Tikhomirova V. V. Barmin Yu. A. Topchu A. M. Mazitova E. Yu. Ratner A. G. Sabirov Z. I. Аbramova A. Ya. Deneka NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC) Сибирский онкологический журнал немелкоклеточный рак легкого общая выживаемость беспрогрессивная выживаемость поддерживающие белки nedd9 маркеры-предикторы |
| title | NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC) |
| title_full | NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC) |
| title_fullStr | NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC) |
| title_full_unstemmed | NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC) |
| title_short | NEDD9 scaffolding protein expression as a negative prediction marker in non-small cell lung cancer (NSCLC) |
| title_sort | nedd9 scaffolding protein expression as a negative prediction marker in non small cell lung cancer nsclc |
| topic | немелкоклеточный рак легкого общая выживаемость беспрогрессивная выживаемость поддерживающие белки nedd9 маркеры-предикторы |
| url | https://www.siboncoj.ru/jour/article/view/2242 |
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