Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus
Summary: Functional compensation in response to gene dysfunction is a fascinating phenomenon that allows mutated viruses to regain the capabilities of their wild-type parental strains. In this study, we isolated mutants of tobacco mosaic virus capable of CP-independent systemic movement. These gain-...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222017588 |
| _version_ | 1811220008755265536 |
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| author | Phu-Tri Tran Mi-Sa Vo Phan Vitaly Citovsky |
| author_facet | Phu-Tri Tran Mi-Sa Vo Phan Vitaly Citovsky |
| author_sort | Phu-Tri Tran |
| collection | DOAJ |
| description | Summary: Functional compensation in response to gene dysfunction is a fascinating phenomenon that allows mutated viruses to regain the capabilities of their wild-type parental strains. In this study, we isolated mutants of tobacco mosaic virus capable of CP-independent systemic movement. These gain-of-function mutants lacked the 16 C-terminal amino acids of the movement protein (MP). Whereas this deletion did not affect the cell-to-cell movement of MP, it dramatically enhanced the viral genomic RNA levels and MP accumulation within the infected cells and altered the subcellular localization of MP from exclusively plasmodesmata (PD) to both PD and plasma membrane. The adapted defective virus suppressed the expression of the ethylene pathway and phloem-associated resistance factors in the inoculated leaves. These findings demonstrate the potential for plant viral MPs to gain a new function that allows viral genomes to move systemically in the absence of the natural viral factor that mediates this spread. |
| first_indexed | 2024-04-12T07:35:16Z |
| format | Article |
| id | doaj.art-fd3990bc9d0d43fc9d54cf57088ec181 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T07:35:16Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-fd3990bc9d0d43fc9d54cf57088ec1812022-12-22T03:41:58ZengElsevieriScience2589-00422022-12-012512105486Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virusPhu-Tri Tran0Mi-Sa Vo Phan1Vitaly Citovsky2Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USA; Corresponding authorDepartment of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USADepartment of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, USASummary: Functional compensation in response to gene dysfunction is a fascinating phenomenon that allows mutated viruses to regain the capabilities of their wild-type parental strains. In this study, we isolated mutants of tobacco mosaic virus capable of CP-independent systemic movement. These gain-of-function mutants lacked the 16 C-terminal amino acids of the movement protein (MP). Whereas this deletion did not affect the cell-to-cell movement of MP, it dramatically enhanced the viral genomic RNA levels and MP accumulation within the infected cells and altered the subcellular localization of MP from exclusively plasmodesmata (PD) to both PD and plasma membrane. The adapted defective virus suppressed the expression of the ethylene pathway and phloem-associated resistance factors in the inoculated leaves. These findings demonstrate the potential for plant viral MPs to gain a new function that allows viral genomes to move systemically in the absence of the natural viral factor that mediates this spread.http://www.sciencedirect.com/science/article/pii/S2589004222017588VirologyCell biologyMolecular plant pathology |
| spellingShingle | Phu-Tri Tran Mi-Sa Vo Phan Vitaly Citovsky Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus iScience Virology Cell biology Molecular plant pathology |
| title | Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus |
| title_full | Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus |
| title_fullStr | Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus |
| title_full_unstemmed | Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus |
| title_short | Gain-of-function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus |
| title_sort | gain of function mutant of movement protein allows systemic transport of a defective tobacco mosaic virus |
| topic | Virology Cell biology Molecular plant pathology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222017588 |
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