Combinations of Spok genes create multiple meiotic drivers in Podospora
Meiotic drive is the preferential transmission of a particular allele during sexual reproduction. The phenomenon is observed as spore killing in multiple fungi. In natural populations of Podospora anserina, seven spore killer types (Psks) have been identified through classical genetic analyses. Here...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/46454 |
| _version_ | 1811236789407449088 |
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| author | Aaron A Vogan S Lorena Ament-Velásquez Alexandra Granger-Farbos Jesper Svedberg Eric Bastiaans Alfons JM Debets Virginie Coustou Hélène Yvanne Corinne Clavé Sven J Saupe Hanna Johannesson |
| author_facet | Aaron A Vogan S Lorena Ament-Velásquez Alexandra Granger-Farbos Jesper Svedberg Eric Bastiaans Alfons JM Debets Virginie Coustou Hélène Yvanne Corinne Clavé Sven J Saupe Hanna Johannesson |
| author_sort | Aaron A Vogan |
| collection | DOAJ |
| description | Meiotic drive is the preferential transmission of a particular allele during sexual reproduction. The phenomenon is observed as spore killing in multiple fungi. In natural populations of Podospora anserina, seven spore killer types (Psks) have been identified through classical genetic analyses. Here we show that the Spok gene family underlies the Psks. The combination of Spok genes at different chromosomal locations defines the spore killer types and creates a killing hierarchy within a population. We identify two novel Spok homologs located within a large (74–167 kbp) region (the Spok block) that resides in different chromosomal locations in different strains. We confirm that the SPOK protein performs both killing and resistance functions and show that these activities are dependent on distinct domains, a predicted nuclease and kinase domain. Genomic and phylogenetic analyses across ascomycetes suggest that the Spok genes disperse through cross-species transfer, and evolve by duplication and diversification within lineages. |
| first_indexed | 2024-04-12T12:15:12Z |
| format | Article |
| id | doaj.art-fd3d01c91e1b4a4387ec3e007d9b8925 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:15:12Z |
| publishDate | 2019-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-fd3d01c91e1b4a4387ec3e007d9b89252022-12-22T03:33:28ZengeLife Sciences Publications LtdeLife2050-084X2019-07-01810.7554/eLife.46454Combinations of Spok genes create multiple meiotic drivers in PodosporaAaron A Vogan0https://orcid.org/0000-0003-2013-7445S Lorena Ament-Velásquez1https://orcid.org/0000-0003-3371-9292Alexandra Granger-Farbos2Jesper Svedberg3Eric Bastiaans4Alfons JM Debets5Virginie Coustou6Hélène Yvanne7Corinne Clavé8Sven J Saupe9Hanna Johannesson10https://orcid.org/0000-0001-6359-9856Organismal biology, Uppsala University, Uppsala, SwedenOrganismal biology, Uppsala University, Uppsala, SwedenUniversity of Bordeaux, Bordeaux, FranceOrganismal biology, Uppsala University, Uppsala, SwedenWageningen University, Wageningen, NetherlandsWageningen University, Wageningen, NetherlandsUniversity of Bordeaux, Bordeaux, FranceUniversity of Bordeaux, Bordeaux, FranceUniversity of Bordeaux, Bordeaux, FranceUniversity of Bordeaux, Bordeaux, FranceOrganismal biology, Uppsala University, Uppsala, SwedenMeiotic drive is the preferential transmission of a particular allele during sexual reproduction. The phenomenon is observed as spore killing in multiple fungi. In natural populations of Podospora anserina, seven spore killer types (Psks) have been identified through classical genetic analyses. Here we show that the Spok gene family underlies the Psks. The combination of Spok genes at different chromosomal locations defines the spore killer types and creates a killing hierarchy within a population. We identify two novel Spok homologs located within a large (74–167 kbp) region (the Spok block) that resides in different chromosomal locations in different strains. We confirm that the SPOK protein performs both killing and resistance functions and show that these activities are dependent on distinct domains, a predicted nuclease and kinase domain. Genomic and phylogenetic analyses across ascomycetes suggest that the Spok genes disperse through cross-species transfer, and evolve by duplication and diversification within lineages.https://elifesciences.org/articles/46454Podosporaselfish genetic elementspore killergenomic conflictfungigene drive |
| spellingShingle | Aaron A Vogan S Lorena Ament-Velásquez Alexandra Granger-Farbos Jesper Svedberg Eric Bastiaans Alfons JM Debets Virginie Coustou Hélène Yvanne Corinne Clavé Sven J Saupe Hanna Johannesson Combinations of Spok genes create multiple meiotic drivers in Podospora eLife Podospora selfish genetic element spore killer genomic conflict fungi gene drive |
| title | Combinations of Spok genes create multiple meiotic drivers in Podospora |
| title_full | Combinations of Spok genes create multiple meiotic drivers in Podospora |
| title_fullStr | Combinations of Spok genes create multiple meiotic drivers in Podospora |
| title_full_unstemmed | Combinations of Spok genes create multiple meiotic drivers in Podospora |
| title_short | Combinations of Spok genes create multiple meiotic drivers in Podospora |
| title_sort | combinations of spok genes create multiple meiotic drivers in podospora |
| topic | Podospora selfish genetic element spore killer genomic conflict fungi gene drive |
| url | https://elifesciences.org/articles/46454 |
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