Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i>
<i>Pestalotiopsis</i> sp. is an important class of plant pathogenic fungi that can infect a variety of crops. We have proved the pathogenicity of <i>P. kenyana</i> on bayberry leaves and caused bayberry blight. Phenazine-1-carboxylic acid (PCA) has the characteristics of high...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/14/11274 |
| _version_ | 1797589078552608768 |
|---|---|
| author | Weizhi Xun Bing Gong Xingxin Liu Xiuju Yang Xia Zhou Linhong Jin |
| author_facet | Weizhi Xun Bing Gong Xingxin Liu Xiuju Yang Xia Zhou Linhong Jin |
| author_sort | Weizhi Xun |
| collection | DOAJ |
| description | <i>Pestalotiopsis</i> sp. is an important class of plant pathogenic fungi that can infect a variety of crops. We have proved the pathogenicity of <i>P. kenyana</i> on bayberry leaves and caused bayberry blight. Phenazine-1-carboxylic acid (PCA) has the characteristics of high efficiency, low toxicity, and environmental friendliness, which can prevent fungal diseases on a variety of crops. In this study, the effect of PCA on the morphological, physiological, and molecular characteristics of <i>P. kenyana</i> has been investigated, and the potential antifungal mechanism of PCA against <i>P. kenyana</i> was also explored. We applied PCA on <i>P. kenyana</i> in vitro and in vivo to determine its inhibitory effect on PCA. It was found that PCA was highly efficient against <i>P. kenyana,</i> with EC<sub>50</sub> around 2.32 μg/mL, and the in vivo effect was 57% at 14 μg/mL. The mechanism of PCA was preliminarily explored by transcriptomics technology. The results showed that after the treatment of PCA, 3613 differential genes were found, focusing on redox processes and various metabolic pathways. In addition, it can also cause mycelial development malformation, damage cell membranes, reduce mitochondrial membrane potential, and increase ROS levels. This result expanded the potential agricultural application of PCA and revealed the possible mechanism against <i>P. kenyana</i>. |
| first_indexed | 2024-03-11T01:02:13Z |
| format | Article |
| id | doaj.art-fd3f2e9b2bd94e28a25531dfcce689fc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:02:13Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fd3f2e9b2bd94e28a25531dfcce689fc2023-11-18T19:36:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141127410.3390/ijms241411274Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i>Weizhi Xun0Bing Gong1Xingxin Liu2Xiuju Yang3Xia Zhou4Linhong Jin5Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaKey Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaKey Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaKey Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaKey Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaKey Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China<i>Pestalotiopsis</i> sp. is an important class of plant pathogenic fungi that can infect a variety of crops. We have proved the pathogenicity of <i>P. kenyana</i> on bayberry leaves and caused bayberry blight. Phenazine-1-carboxylic acid (PCA) has the characteristics of high efficiency, low toxicity, and environmental friendliness, which can prevent fungal diseases on a variety of crops. In this study, the effect of PCA on the morphological, physiological, and molecular characteristics of <i>P. kenyana</i> has been investigated, and the potential antifungal mechanism of PCA against <i>P. kenyana</i> was also explored. We applied PCA on <i>P. kenyana</i> in vitro and in vivo to determine its inhibitory effect on PCA. It was found that PCA was highly efficient against <i>P. kenyana,</i> with EC<sub>50</sub> around 2.32 μg/mL, and the in vivo effect was 57% at 14 μg/mL. The mechanism of PCA was preliminarily explored by transcriptomics technology. The results showed that after the treatment of PCA, 3613 differential genes were found, focusing on redox processes and various metabolic pathways. In addition, it can also cause mycelial development malformation, damage cell membranes, reduce mitochondrial membrane potential, and increase ROS levels. This result expanded the potential agricultural application of PCA and revealed the possible mechanism against <i>P. kenyana</i>.https://www.mdpi.com/1422-0067/24/14/11274bayberry diseasebioactivitymechanism<i>Pestalotiopsis kenyana</i>transcriptomics analysesbiogenic fungicide |
| spellingShingle | Weizhi Xun Bing Gong Xingxin Liu Xiuju Yang Xia Zhou Linhong Jin Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i> International Journal of Molecular Sciences bayberry disease bioactivity mechanism <i>Pestalotiopsis kenyana</i> transcriptomics analyses biogenic fungicide |
| title | Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i> |
| title_full | Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i> |
| title_fullStr | Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i> |
| title_full_unstemmed | Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i> |
| title_short | Antifungal Mechanism of Phenazine-1-Carboxylic Acid against <i>Pestalotiopsis kenyana</i> |
| title_sort | antifungal mechanism of phenazine 1 carboxylic acid against i pestalotiopsis kenyana i |
| topic | bayberry disease bioactivity mechanism <i>Pestalotiopsis kenyana</i> transcriptomics analyses biogenic fungicide |
| url | https://www.mdpi.com/1422-0067/24/14/11274 |
| work_keys_str_mv | AT weizhixun antifungalmechanismofphenazine1carboxylicacidagainstipestalotiopsiskenyanai AT binggong antifungalmechanismofphenazine1carboxylicacidagainstipestalotiopsiskenyanai AT xingxinliu antifungalmechanismofphenazine1carboxylicacidagainstipestalotiopsiskenyanai AT xiujuyang antifungalmechanismofphenazine1carboxylicacidagainstipestalotiopsiskenyanai AT xiazhou antifungalmechanismofphenazine1carboxylicacidagainstipestalotiopsiskenyanai AT linhongjin antifungalmechanismofphenazine1carboxylicacidagainstipestalotiopsiskenyanai |