Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen Supplementation
Bee pollen, because of its high content of nutrients, is a very valuable medicinal and nutritional product. However, since its composition is not completely studied, the consumption of this product may cause adverse effects, including allergic reactions. Therefore, this study aimed to discover and c...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/22/7733 |
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| author | Eliza Matuszewska Szymon Plewa Dagmara Pietkiewicz Kacper Kossakowski Joanna Matysiak Grzegorz Rosiński Jan Matysiak |
| author_facet | Eliza Matuszewska Szymon Plewa Dagmara Pietkiewicz Kacper Kossakowski Joanna Matysiak Grzegorz Rosiński Jan Matysiak |
| author_sort | Eliza Matuszewska |
| collection | DOAJ |
| description | Bee pollen, because of its high content of nutrients, is a very valuable medicinal and nutritional product. However, since its composition is not completely studied, the consumption of this product may cause adverse effects, including allergic reactions. Therefore, this study aimed to discover and characterize the bioactive proteins of bee pollen collected in Poland, focusing mainly on the allergens. For this purpose, the purified and concentrated pollen aqueous solutions were analyzed using the nanoLC-MALDI-TOF/TOF MS analytical platform. As a result of the experiments, 197 unique proteins derived from green plants (<i>Viridiplantae</i>) and 10 unique proteins derived from bees (<i>Apis</i> spp.) were identified. Among them, potential plant allergens were discovered. Moreover, proteins belonging to the group of hypothetical proteins, whose expression had not been confirmed experimentally before, were detected. Because of the content of bioactive compounds—both beneficial and harmful—there is a critical need to develop guidelines for standardizing bee pollen, especially intended for consumption or therapeutic purposes. This is of particular importance because awareness of the allergen content of bee pollen and other bee products can prevent health- or life-threatening incidents following the ingestion of these increasingly popular “superfoods”. |
| first_indexed | 2024-03-09T18:08:30Z |
| format | Article |
| id | doaj.art-fd436b1110b643f7b3a0bd754495a52e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:08:30Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fd436b1110b643f7b3a0bd754495a52e2023-11-24T09:20:15ZengMDPI AGMolecules1420-30492022-11-012722773310.3390/molecules27227733Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen SupplementationEliza Matuszewska0Szymon Plewa1Dagmara Pietkiewicz2Kacper Kossakowski3Joanna Matysiak4Grzegorz Rosiński5Jan Matysiak6Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 3 Rokietnicka Street, 60-806 Poznań, PolandDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 3 Rokietnicka Street, 60-806 Poznań, PolandDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 3 Rokietnicka Street, 60-806 Poznań, PolandDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 3 Rokietnicka Street, 60-806 Poznań, PolandFaculty of Health Sciences, Calisia University, 13 Kaszubska Street, 62-800 Kalisz, PolandDepartment of Animal Physiology and Development, Faculty of Biology, Adam Mickiewicz University in Poznan, 6 Uniwersytetu Poznańskiego Street, 61-614 Poznań, PolandDepartment of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 3 Rokietnicka Street, 60-806 Poznań, PolandBee pollen, because of its high content of nutrients, is a very valuable medicinal and nutritional product. However, since its composition is not completely studied, the consumption of this product may cause adverse effects, including allergic reactions. Therefore, this study aimed to discover and characterize the bioactive proteins of bee pollen collected in Poland, focusing mainly on the allergens. For this purpose, the purified and concentrated pollen aqueous solutions were analyzed using the nanoLC-MALDI-TOF/TOF MS analytical platform. As a result of the experiments, 197 unique proteins derived from green plants (<i>Viridiplantae</i>) and 10 unique proteins derived from bees (<i>Apis</i> spp.) were identified. Among them, potential plant allergens were discovered. Moreover, proteins belonging to the group of hypothetical proteins, whose expression had not been confirmed experimentally before, were detected. Because of the content of bioactive compounds—both beneficial and harmful—there is a critical need to develop guidelines for standardizing bee pollen, especially intended for consumption or therapeutic purposes. This is of particular importance because awareness of the allergen content of bee pollen and other bee products can prevent health- or life-threatening incidents following the ingestion of these increasingly popular “superfoods”.https://www.mdpi.com/1420-3049/27/22/7733allergensbee pollenMALDI-TOFmass spectrometryproteomics |
| spellingShingle | Eliza Matuszewska Szymon Plewa Dagmara Pietkiewicz Kacper Kossakowski Joanna Matysiak Grzegorz Rosiński Jan Matysiak Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen Supplementation Molecules allergens bee pollen MALDI-TOF mass spectrometry proteomics |
| title | Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen Supplementation |
| title_full | Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen Supplementation |
| title_fullStr | Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen Supplementation |
| title_full_unstemmed | Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen Supplementation |
| title_short | Mass Spectrometry-Based Identification of Bioactive Bee Pollen Proteins: Evaluation of Allergy Risk after Bee Pollen Supplementation |
| title_sort | mass spectrometry based identification of bioactive bee pollen proteins evaluation of allergy risk after bee pollen supplementation |
| topic | allergens bee pollen MALDI-TOF mass spectrometry proteomics |
| url | https://www.mdpi.com/1420-3049/27/22/7733 |
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