Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico
SARS-CoV-2 mainly affects humans; however, it is important to monitor the infection of companion and wild animals as possible reservoirs of this virus. In this sense, seroprevalence studies in companion animals, such as dogs and cats, provide important information about the epidemiology of SARS-CoV-...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/12/6/835 |
| _version_ | 1797593152671973376 |
|---|---|
| author | Freddy Dehesa-Canseco Roxana Pastrana-Unzueta Nadia Carrillo-Guzmán Francisco Liljehult-Fuentes Juan Diego Pérez-De la Rosa Humberto Ramírez-Mendoza Jose Guillermo Estrada-Franco Roberto Navarro-López Jesús Hernández Mario Solís-Hernández |
| author_facet | Freddy Dehesa-Canseco Roxana Pastrana-Unzueta Nadia Carrillo-Guzmán Francisco Liljehult-Fuentes Juan Diego Pérez-De la Rosa Humberto Ramírez-Mendoza Jose Guillermo Estrada-Franco Roberto Navarro-López Jesús Hernández Mario Solís-Hernández |
| author_sort | Freddy Dehesa-Canseco |
| collection | DOAJ |
| description | SARS-CoV-2 mainly affects humans; however, it is important to monitor the infection of companion and wild animals as possible reservoirs of this virus. In this sense, seroprevalence studies in companion animals, such as dogs and cats, provide important information about the epidemiology of SARS-CoV-2. This study aimed to evaluate the seroprevalence of neutralizing antibodies (nAbs) against the ancestral strain and the Omicron BA.1 subvariant in dogs and cats in Mexico. Six hundred and two samples were obtained from dogs (n = 574) and cats (n = 28). These samples were collected from the end of 2020 to December 2021 from different regions of Mexico. The presence of nAbs was evaluated using a plaque reduction neutralization test (PRNT) and microneutralization (MN) assays. The results showed that 14.2% of cats and 1.5% of dogs presented nAbs against the ancestral strain of SARS-CoV-2. The analysis of nAbs against Omicron BA.1 in cats showed the same percentage of positive animals but a reduced titer. In dogs, 1.2% showed nAbs against Omicron BA.1. These results indicate that nAbs were more frequent in cats than in dogs and that these nAbs have a lower capacity to neutralize the subvariant Omicron BA.1. |
| first_indexed | 2024-03-11T02:04:52Z |
| format | Article |
| id | doaj.art-fd442781e409447088ba7a9b42336336 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T02:04:52Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fd442781e409447088ba7a9b423363362023-11-18T12:00:37ZengMDPI AGPathogens2076-08172023-06-0112683510.3390/pathogens12060835Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in MexicoFreddy Dehesa-Canseco0Roxana Pastrana-Unzueta1Nadia Carrillo-Guzmán2Francisco Liljehult-Fuentes3Juan Diego Pérez-De la Rosa4Humberto Ramírez-Mendoza5Jose Guillermo Estrada-Franco6Roberto Navarro-López7Jesús Hernández8Mario Solís-Hernández9Comisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales (CPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Ciudad de Mexico 05110, MexicoComisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales (CPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Ciudad de Mexico 05110, MexicoComisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales (CPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Ciudad de Mexico 05110, MexicoComisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales (CPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Ciudad de Mexico 05110, MexicoCentro Nacional de Servicios de Constatación en Salud Animal (CENAPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Cuernavaca 62574, MexicoDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autónoma de México (UNAM), Ciudad de Mexico 04510, MexicoCentro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa 88710, MexicoComisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales (CPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Ciudad de Mexico 05110, MexicoLaboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A. C. (CIAD), Hermosillo Sonora 83304, MexicoComisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los Animales (CPA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA), Secretaría de Agricultura y Desarrollo Rural (SADER), Ciudad de Mexico 05110, MexicoSARS-CoV-2 mainly affects humans; however, it is important to monitor the infection of companion and wild animals as possible reservoirs of this virus. In this sense, seroprevalence studies in companion animals, such as dogs and cats, provide important information about the epidemiology of SARS-CoV-2. This study aimed to evaluate the seroprevalence of neutralizing antibodies (nAbs) against the ancestral strain and the Omicron BA.1 subvariant in dogs and cats in Mexico. Six hundred and two samples were obtained from dogs (n = 574) and cats (n = 28). These samples were collected from the end of 2020 to December 2021 from different regions of Mexico. The presence of nAbs was evaluated using a plaque reduction neutralization test (PRNT) and microneutralization (MN) assays. The results showed that 14.2% of cats and 1.5% of dogs presented nAbs against the ancestral strain of SARS-CoV-2. The analysis of nAbs against Omicron BA.1 in cats showed the same percentage of positive animals but a reduced titer. In dogs, 1.2% showed nAbs against Omicron BA.1. These results indicate that nAbs were more frequent in cats than in dogs and that these nAbs have a lower capacity to neutralize the subvariant Omicron BA.1.https://www.mdpi.com/2076-0817/12/6/835catsCOVID-19dogsmicroneutralizationPRNT-90SARS-CoV-2 |
| spellingShingle | Freddy Dehesa-Canseco Roxana Pastrana-Unzueta Nadia Carrillo-Guzmán Francisco Liljehult-Fuentes Juan Diego Pérez-De la Rosa Humberto Ramírez-Mendoza Jose Guillermo Estrada-Franco Roberto Navarro-López Jesús Hernández Mario Solís-Hernández Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico Pathogens cats COVID-19 dogs microneutralization PRNT-90 SARS-CoV-2 |
| title | Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico |
| title_full | Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico |
| title_fullStr | Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico |
| title_full_unstemmed | Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico |
| title_short | Neutralizing Antibodies against the SARS-CoV-2 Ancestral Strain and Omicron BA.1 Subvariant in Dogs and Cats in Mexico |
| title_sort | neutralizing antibodies against the sars cov 2 ancestral strain and omicron ba 1 subvariant in dogs and cats in mexico |
| topic | cats COVID-19 dogs microneutralization PRNT-90 SARS-CoV-2 |
| url | https://www.mdpi.com/2076-0817/12/6/835 |
| work_keys_str_mv | AT freddydehesacanseco neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT roxanapastranaunzueta neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT nadiacarrilloguzman neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT franciscoliljehultfuentes neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT juandiegoperezdelarosa neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT humbertoramirezmendoza neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT joseguillermoestradafranco neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT robertonavarrolopez neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT jesushernandez neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico AT mariosolishernandez neutralizingantibodiesagainstthesarscov2ancestralstrainandomicronba1subvariantindogsandcatsinmexico |