The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral Tongue
Background and objective: Oral tongue Squamous Cell carcinoma (SCC) commonly involves males between the sixth to eighth decades of life. Major risk factors are tobacco usage and alcohol consumption. The increasing number of patients developing oral tongue cancer without these well-known risk factor...
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| Format: | Article |
| Language: | English |
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Iranian Society of Pathology
2017-04-01
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| Series: | Iranian Journal of Pathology |
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| Online Access: | https://ijp.iranpath.org/article_24872_44c201740c762b8a2e9be341c81e5e1d.pdf |
| _version_ | 1819096952431706112 |
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| author | Mohamad Javad Ashraf Shahla Hosseini Ahmad Monabati Behnaz Valibeigi Bijan Khademi Elham Abedi Negar Azarpira |
| author_facet | Mohamad Javad Ashraf Shahla Hosseini Ahmad Monabati Behnaz Valibeigi Bijan Khademi Elham Abedi Negar Azarpira |
| author_sort | Mohamad Javad Ashraf |
| collection | DOAJ |
| description | Background and objective: Oral tongue Squamous Cell carcinoma (SCC) commonly involves males between the sixth to eighth decades of life. Major risk factors are tobacco usage and alcohol consumption. The increasing number of patients developing oral tongue cancer without these well-known risk factors suggests that a viral infection, such as Human Papillomavirus (HPV), may be responsible for this increase, by acting as an oncogenic agent. This study investigated the prevalence of HPV infection and its clinicopathologic significance in oral tongue SCCs. Material and methods: Tissue blocks from a total of 50 cases (patients with oral tongue SCC) and 50 controls (palatine tonsillar tissues with benign diagnosis) were selected. DNA was extracted from tumoral and non-tumoral tissue blocks. Detection of common HPV DNA by nested Polymerase Chain Reaction (PCR), and high-risk genotypes, HPV 16 and HPV 18, by conventional PCR, was achieved and the results correlated with clinicopathological parameters. Results: Of the 50 patients (18 males and 32 females with a mean age of 57.36±12.18 years, and age range of 27 to 86 years), 7 (14%) had HPV positive results. None of the control group subjects had HPV DNA positive results (P-value of 0.012). The HPV genotype 16/18 was not detected in positive cases. No statistically significant association was found between HPV status and gender, age, tumor grade, tumor stage or lymph node involvement. Conclusion: Although there was a significantly higher prevalence of HPV in oral tongue SCC, its association with carcinogenesis in this area requires further studies. |
| first_indexed | 2024-12-22T00:07:22Z |
| format | Article |
| id | doaj.art-fd49b3dacb9840b6b54d073a0ac399e7 |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-22T00:07:22Z |
| publishDate | 2017-04-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-fd49b3dacb9840b6b54d073a0ac399e72022-12-21T18:45:32ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562017-04-0112214414910.30699/ijp.2017.2487224872The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral TongueMohamad Javad Ashraf0Shahla Hosseini1Ahmad Monabati2Behnaz Valibeigi3Bijan Khademi4Elham Abedi5Negar Azarpira6Pathology Department, Shiraz University of Medical Sciences, Shiraz, IranPathology Department, Shiraz University of Medical Sciences, Shiraz, IranPathology Department, Shiraz University of Medical Sciences, Shiraz, IranPathology Department, Shiraz University of Medical Sciences, Shiraz, IranOtolaryngology Department, Shiraz University of Medical Sciences, Shiraz, IranPathology Department, Shiraz University of Medical Sciences, Shiraz, IranTransplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranBackground and objective: Oral tongue Squamous Cell carcinoma (SCC) commonly involves males between the sixth to eighth decades of life. Major risk factors are tobacco usage and alcohol consumption. The increasing number of patients developing oral tongue cancer without these well-known risk factors suggests that a viral infection, such as Human Papillomavirus (HPV), may be responsible for this increase, by acting as an oncogenic agent. This study investigated the prevalence of HPV infection and its clinicopathologic significance in oral tongue SCCs. Material and methods: Tissue blocks from a total of 50 cases (patients with oral tongue SCC) and 50 controls (palatine tonsillar tissues with benign diagnosis) were selected. DNA was extracted from tumoral and non-tumoral tissue blocks. Detection of common HPV DNA by nested Polymerase Chain Reaction (PCR), and high-risk genotypes, HPV 16 and HPV 18, by conventional PCR, was achieved and the results correlated with clinicopathological parameters. Results: Of the 50 patients (18 males and 32 females with a mean age of 57.36±12.18 years, and age range of 27 to 86 years), 7 (14%) had HPV positive results. None of the control group subjects had HPV DNA positive results (P-value of 0.012). The HPV genotype 16/18 was not detected in positive cases. No statistically significant association was found between HPV status and gender, age, tumor grade, tumor stage or lymph node involvement. Conclusion: Although there was a significantly higher prevalence of HPV in oral tongue SCC, its association with carcinogenesis in this area requires further studies.https://ijp.iranpath.org/article_24872_44c201740c762b8a2e9be341c81e5e1d.pdfhuman papilloma virussquamous cell carcinomatongue |
| spellingShingle | Mohamad Javad Ashraf Shahla Hosseini Ahmad Monabati Behnaz Valibeigi Bijan Khademi Elham Abedi Negar Azarpira The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral Tongue Iranian Journal of Pathology human papilloma virus squamous cell carcinoma tongue |
| title | The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral Tongue |
| title_full | The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral Tongue |
| title_fullStr | The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral Tongue |
| title_full_unstemmed | The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral Tongue |
| title_short | The Prevalence of Human Papilloma Virus in Squamous Cell Carcinoma of Oral Tongue |
| title_sort | prevalence of human papilloma virus in squamous cell carcinoma of oral tongue |
| topic | human papilloma virus squamous cell carcinoma tongue |
| url | https://ijp.iranpath.org/article_24872_44c201740c762b8a2e9be341c81e5e1d.pdf |
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