| Summary: | Effects of landscape patterns or topographic features on the river water environment have been broadly studied to control non-point source (NPS) pollution and to cut off potential pathways for pollutants to affect human health. However, spatio-temporal dynamics and scale effects with respect to the impact of landscape patterns and topographic features on the aquatic environment over successive years have not been elucidated. In this study, water quality parameters and land cover data for three consecutive years mainly in Tangshan City, located in the northeast of the Haihe River Basin, China, were obtained to determine the associations between landscape patterns and topographic features with the water environment. Results indicated that seasonal differences in dissolved oxygen (DO) and total nitrogen (TN) were significant (<i>p</i> < 0.001), and spatial variation was generally observed for each water quality parameter. Redundancy analysis revealed that landscape patterns and topographic features have different impacts on the aquatic environment as seasonal spans and spatial scales change. Overall, the best explanatory variables explained an average of 58.6% of the variation in water quality at various spatial scales over the two seasons. Topographic features made a greater contribution to river water quality changes at the buffer scale; conversely, at the catchment scale, water quality changes stemmed primarily from differences in landscape composition and configuration. The landscape shape index of cropland (LSI<sub>crop</sub>) was an important factor influencing seasonal river water quality changes at various spatial scales. These results suggest that considering landscape connectivity at distinct spatial scales could enhance the understanding of the alteration of hydrological processes across multiple topographic features, which in turn has an impact on seasonal river water.
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