Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定)

Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定)

建立HPLC法测定桑叶提取物中水解槲皮素和山萘酚的含量.先用甲醇-盐酸混合液（甲醇终浓度50%,盐酸终浓度2.0 mol •L-1）水解桑叶提取物中的黄酮类成分成槲皮素和山萘酚,以HPLC法测定槲皮素和山萘酚含量.色谱柱为Diamonsil钻石C18柱,流动相为甲醇-0.2%磷酸（63 ：37 ,体积分数）,流速为1.0 mL •min-1,检测波长为370 nm.结果表明槲皮素在0.84~26.8 mg •L -1之间,山萘酚在0.44~14.2 mg •L -1之间呈良好的线性关系,平均回收率分别为101.3%和99.5%.该测定方法准确、重复性好,可用于桑叶提取物中槲皮素和山萘酚的含量测...

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Bibliographic Details
Main Authors: JIANGLi-di(蒋立娣), XUANGui-da(宣贵达), WUHao-hao(吴好好), LILi-ping(李丽萍)
Format: Article
Language:zho
Published: Zhejiang University Press 2009-11-01
Series:Zhejiang Daxue xuebao. Lixue ban
Subjects:
槲皮素
山萘酚
高效液相色谱法
桑叶提取物
Online Access:https://doi.org/10.3785/j.issn.1008-9497.2009.06.023
Description
Summary:建立HPLC法测定桑叶提取物中水解槲皮素和山萘酚的含量.先用甲醇-盐酸混合液（甲醇终浓度50%,盐酸终浓度2.0 mol •L-1）水解桑叶提取物中的黄酮类成分成槲皮素和山萘酚,以HPLC法测定槲皮素和山萘酚含量.色谱柱为Diamonsil钻石C18柱,流动相为甲醇-0.2%磷酸（63 ：37 ,体积分数）,流速为1.0 mL •min-1,检测波长为370 nm.结果表明槲皮素在0.84~26.8 mg •L -1之间,山萘酚在0.44~14.2 mg •L -1之间呈良好的线性关系,平均回收率分别为101.3%和99.5%.该测定方法准确、重复性好,可用于桑叶提取物中槲皮素和山萘酚的含量测定.
ISSN:1008-9497

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