Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定)
建立HPLC法测定桑叶提取物中水解槲皮素和山萘酚的含量.先用甲醇-盐酸混合液（甲醇终浓度50%,盐酸终浓度2.0 mol •L-1）水解桑叶提取物中的黄酮类成分成槲皮素和山萘酚,以HPLC法测定槲皮素和山萘酚含量.色谱柱为Diamonsil钻石C18柱,流动相为甲醇-0.2%磷酸（63 ：37 ,体积分数）,流速为1.0 mL •min-1,检测波长为370 nm.结果表明槲皮素在0.84~26.8 mg •L -1之间,山萘酚在0.44~14.2 mg •L -1之间呈良好的线性关系,平均回收率分别为101.3%和99.5%.该测定方法准确、重复性好,可用于桑叶提取物中槲皮素和山萘酚的含量测...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2009-11-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2009.06.023 |
| _version_ | 1827303357771939840 |
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| author | JIANGLi-di(蒋立娣) XUANGui-da(宣贵达) WUHao-hao(吴好好) LILi-ping(李丽萍) |
| author_facet | JIANGLi-di(蒋立娣) XUANGui-da(宣贵达) WUHao-hao(吴好好) LILi-ping(李丽萍) |
| author_sort | JIANGLi-di(蒋立娣) |
| collection | DOAJ |
| description | 建立HPLC法测定桑叶提取物中水解槲皮素和山萘酚的含量.先用甲醇-盐酸混合液（甲醇终浓度50%,盐酸终浓度2.0 mol •L-1）水解桑叶提取物中的黄酮类成分成槲皮素和山萘酚,以HPLC法测定槲皮素和山萘酚含量.色谱柱为Diamonsil钻石C18柱,流动相为甲醇-0.2%磷酸（63 ：37 ,体积分数）,流速为1.0 mL •min-1,检测波长为370 nm.结果表明槲皮素在0.84~26.8 mg •L -1之间,山萘酚在0.44~14.2 mg •L -1之间呈良好的线性关系,平均回收率分别为101.3%和99.5%.该测定方法准确、重复性好,可用于桑叶提取物中槲皮素和山萘酚的含量测定. |
| first_indexed | 2024-04-24T17:00:09Z |
| format | Article |
| id | doaj.art-fd4e64c92252468e81bc40aa1acac409 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:00:09Z |
| publishDate | 2009-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-fd4e64c92252468e81bc40aa1acac4092024-03-29T01:58:27ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972009-11-0136670570710.3785/j.issn.1008-9497.2009.06.023Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定)JIANGLi-di(蒋立娣)0XUANGui-da(宣贵达)1WUHao-hao(吴好好)2LILi-ping(李丽萍)3 1.Pharmaceutical College of Zhejiang University, Hangzhou 310058, China( 1.浙江大学药学院，浙江 杭州 310058) 2.City College of Zhejiang University, Hangzhou 310015, China( 2.浙江大学城市学院，浙江 杭州 310015) 1.Pharmaceutical College of Zhejiang University, Hangzhou 310058, China( 1.浙江大学药学院，浙江 杭州 310058) 1.Pharmaceutical College of Zhejiang University, Hangzhou 310058, China( 1.浙江大学药学院，浙江 杭州 310058)建立HPLC法测定桑叶提取物中水解槲皮素和山萘酚的含量.先用甲醇-盐酸混合液（甲醇终浓度50%,盐酸终浓度2.0 mol •L-1）水解桑叶提取物中的黄酮类成分成槲皮素和山萘酚,以HPLC法测定槲皮素和山萘酚含量.色谱柱为Diamonsil钻石C18柱,流动相为甲醇-0.2%磷酸（63 ：37 ,体积分数）,流速为1.0 mL •min-1,检测波长为370 nm.结果表明槲皮素在0.84~26.8 mg •L -1之间,山萘酚在0.44~14.2 mg •L -1之间呈良好的线性关系,平均回收率分别为101.3%和99.5%.该测定方法准确、重复性好,可用于桑叶提取物中槲皮素和山萘酚的含量测定.https://doi.org/10.3785/j.issn.1008-9497.2009.06.023槲皮素山萘酚高效液相色谱法桑叶提取物 |
| spellingShingle | JIANGLi-di(蒋立娣) XUANGui-da(宣贵达) WUHao-hao(吴好好) LILi-ping(李丽萍) Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定) Zhejiang Daxue xuebao. Lixue ban 槲皮素 山萘酚 高效液相色谱法 桑叶提取物 |
| title | Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定) |
| title_full | Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定) |
| title_fullStr | Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定) |
| title_full_unstemmed | Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定) |
| title_short | Determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid(桑叶提取物中槲皮素和山萘酚的含量测定) |
| title_sort | determination of quercetin and kaempferol in folium mori extract after hydrolysis by hydrochloric acid 桑叶提取物中槲皮素和山萘酚的含量测定 |
| topic | 槲皮素 山萘酚 高效液相色谱法 桑叶提取物 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2009.06.023 |
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