Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer
Abstract Spindle and kinetochore-associated complex subunit 3 (SKA3) is a microtubule-binding subcomplex of the outer kinetochore that is required for proper chromosomal segregation and cell division. However, little is known regarding the probable mechanism of SKA3, particularly in terms of prostat...
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | Experimental Hematology & Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40164-022-00337-3 |
| _version_ | 1797986067441254400 |
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| author | Dechao Feng Weizhen Zhu Xu Shi Qiao Xiong Dengxiong Li Wuran Wei Ping Han Qiang Wei Lu Yang |
| author_facet | Dechao Feng Weizhen Zhu Xu Shi Qiao Xiong Dengxiong Li Wuran Wei Ping Han Qiang Wei Lu Yang |
| author_sort | Dechao Feng |
| collection | DOAJ |
| description | Abstract Spindle and kinetochore-associated complex subunit 3 (SKA3) is a microtubule-binding subcomplex of the outer kinetochore that is required for proper chromosomal segregation and cell division. However, little is known regarding the probable mechanism of SKA3, particularly in terms of prostate cancer (PCA) progression. Multiple databases, including TCGA and GTEx, were utilized to examine the expression of SKA3 in PCA patients and to shed light on the clinical significance and potential mechanism of SKA3 in the onset and progression of PCA. The biological function of SKA3 was evaluated in vitro using RT–qPCR and the CCK8 assay. For statistical analysis, the R 3.6.3 software and its associated packages were utilized. SKA3 was shown to be considerably elevated in PCA patients and was linked to a shorter progress free interval (PFI). Furthermore, we discovered that SKA3 mRNA expression was higher in PCA cells than in normal cells, and inhibition of SKA3 could clearly reduce PCA cell proliferation using the CCK8 assay. Finally, SKA3 could be used as a predictive biomarker in PCA patients. |
| first_indexed | 2024-04-11T07:27:06Z |
| format | Article |
| id | doaj.art-fd533a55564945359f3de729c1584f0d |
| institution | Directory Open Access Journal |
| issn | 2162-3619 |
| language | English |
| last_indexed | 2024-04-11T07:27:06Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Experimental Hematology & Oncology |
| spelling | doaj.art-fd533a55564945359f3de729c1584f0d2022-12-22T04:37:02ZengBMCExperimental Hematology & Oncology2162-36192022-10-011111510.1186/s40164-022-00337-3Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancerDechao Feng0Weizhen Zhu1Xu Shi2Qiao Xiong3Dengxiong Li4Wuran Wei5Ping Han6Qiang Wei7Lu Yang8Department of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityDepartment of Urology, Institute of Urology, West China Hospital, Sichuan UniversityAbstract Spindle and kinetochore-associated complex subunit 3 (SKA3) is a microtubule-binding subcomplex of the outer kinetochore that is required for proper chromosomal segregation and cell division. However, little is known regarding the probable mechanism of SKA3, particularly in terms of prostate cancer (PCA) progression. Multiple databases, including TCGA and GTEx, were utilized to examine the expression of SKA3 in PCA patients and to shed light on the clinical significance and potential mechanism of SKA3 in the onset and progression of PCA. The biological function of SKA3 was evaluated in vitro using RT–qPCR and the CCK8 assay. For statistical analysis, the R 3.6.3 software and its associated packages were utilized. SKA3 was shown to be considerably elevated in PCA patients and was linked to a shorter progress free interval (PFI). Furthermore, we discovered that SKA3 mRNA expression was higher in PCA cells than in normal cells, and inhibition of SKA3 could clearly reduce PCA cell proliferation using the CCK8 assay. Finally, SKA3 could be used as a predictive biomarker in PCA patients.https://doi.org/10.1186/s40164-022-00337-3Spindle and kinetochore associated complex subunit 3Prostate cancerBiomarkerEnrichment analysisTargeted therapy |
| spellingShingle | Dechao Feng Weizhen Zhu Xu Shi Qiao Xiong Dengxiong Li Wuran Wei Ping Han Qiang Wei Lu Yang Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer Experimental Hematology & Oncology Spindle and kinetochore associated complex subunit 3 Prostate cancer Biomarker Enrichment analysis Targeted therapy |
| title | Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer |
| title_full | Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer |
| title_fullStr | Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer |
| title_full_unstemmed | Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer |
| title_short | Spindle and kinetochore-associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer |
| title_sort | spindle and kinetochore associated complex subunit 3 could serve as a prognostic biomarker for prostate cancer |
| topic | Spindle and kinetochore associated complex subunit 3 Prostate cancer Biomarker Enrichment analysis Targeted therapy |
| url | https://doi.org/10.1186/s40164-022-00337-3 |
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