Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE)
Implant insertion can evoke excessive inflammation which disrupts the healing process and potentially leads to complications such as implant rejection. Neutrophils and macrophages play a vital role in the early inflammatory phase of tissue repair, necessitating the study of cellular responses in hos...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Materials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1996-1944/16/16/5691 |
| _version_ | 1797584003951230976 |
|---|---|
| author | Beata Drzewiecka Agata Przekora Dominika Dobko Aleksandra Kozera Katarzyna Krać Dominika Nguyen Ngoc Eric Fernández-De la Cruz Joanna Wessely-Szponder |
| author_facet | Beata Drzewiecka Agata Przekora Dominika Dobko Aleksandra Kozera Katarzyna Krać Dominika Nguyen Ngoc Eric Fernández-De la Cruz Joanna Wessely-Szponder |
| author_sort | Beata Drzewiecka |
| collection | DOAJ |
| description | Implant insertion can evoke excessive inflammation which disrupts the healing process and potentially leads to complications such as implant rejection. Neutrophils and macrophages play a vital role in the early inflammatory phase of tissue repair, necessitating the study of cellular responses in host–implant interactions. In order to deepen the knowledge about these interactions, the response of neutrophils and macrophages to contact with selected biomaterials was examined in vitro on the basis of secretory response as well as reactive oxygen species/reactive nitrogen species (ROS/RNS) generation. Porcine neutrophils exposed to hydroxyapatite (HA) released more enzymes and generated higher levels of ROS/RNS compared to the control group. The addition of AMPNE diminished these responses. Although the results from porcine cells can provide valuable preliminary data, further validation using human cells or clinical studies would be necessary to fully extrapolate the findings to human medicine. Our study revealed that human neutrophils after contact of with HA increased the production of nitric oxide (NO) (10.00 ± 0.08 vs. control group 3.0 ± 0.11 µM, <i>p</i> < 0.05), while HAP or FAP did not elicit a significant response. Human macrophages cultured with HA produced more superoxide and NO, while HAP or FAP had a minimal effect, and curdlan reduced ROS/RNS generation. The addition of AMPNE to cultures with all biomaterials, except curdlan, reduced neutrophil activity, regardless of the peptides’ origin. These results highlight the potential of antimicrobial peptides in modulating excessive biomaterial/host cell reactions involving neutrophils and macrophages, enhancing our understanding of immune reactions, and suggesting that AMPNE could regulate leukocyte response during implantation. |
| first_indexed | 2024-03-10T23:47:05Z |
| format | Article |
| id | doaj.art-fd5409ccba6c4e029757f82a14ccc6de |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T23:47:05Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-fd5409ccba6c4e029757f82a14ccc6de2023-11-19T02:01:23ZengMDPI AGMaterials1996-19442023-08-011616569110.3390/ma16165691Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE)Beata Drzewiecka0Agata Przekora1Dominika Dobko2Aleksandra Kozera3Katarzyna Krać4Dominika Nguyen Ngoc5Eric Fernández-De la Cruz6Joanna Wessely-Szponder7Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, PolandIndependent Unit of Tissue Engineering and Regenerative Medicine, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, PolandStudents Research Group of Veterinary Analysts, Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, PolandStudents Research Group of Veterinary Analysts, Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, PolandStudents Research Group of Veterinary Analysts, Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, PolandSub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, PolandDepartment of Pathology & Experimental Therapeutics, Faculty of Medicine & Health Sciences, University of Barcelona, 08907 Barcelona, SpainSub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, PolandImplant insertion can evoke excessive inflammation which disrupts the healing process and potentially leads to complications such as implant rejection. Neutrophils and macrophages play a vital role in the early inflammatory phase of tissue repair, necessitating the study of cellular responses in host–implant interactions. In order to deepen the knowledge about these interactions, the response of neutrophils and macrophages to contact with selected biomaterials was examined in vitro on the basis of secretory response as well as reactive oxygen species/reactive nitrogen species (ROS/RNS) generation. Porcine neutrophils exposed to hydroxyapatite (HA) released more enzymes and generated higher levels of ROS/RNS compared to the control group. The addition of AMPNE diminished these responses. Although the results from porcine cells can provide valuable preliminary data, further validation using human cells or clinical studies would be necessary to fully extrapolate the findings to human medicine. Our study revealed that human neutrophils after contact of with HA increased the production of nitric oxide (NO) (10.00 ± 0.08 vs. control group 3.0 ± 0.11 µM, <i>p</i> < 0.05), while HAP or FAP did not elicit a significant response. Human macrophages cultured with HA produced more superoxide and NO, while HAP or FAP had a minimal effect, and curdlan reduced ROS/RNS generation. The addition of AMPNE to cultures with all biomaterials, except curdlan, reduced neutrophil activity, regardless of the peptides’ origin. These results highlight the potential of antimicrobial peptides in modulating excessive biomaterial/host cell reactions involving neutrophils and macrophages, enhancing our understanding of immune reactions, and suggesting that AMPNE could regulate leukocyte response during implantation.https://www.mdpi.com/1996-1944/16/16/5691antimicrobial peptidesbiomaterialblood derived productsinflammationmacrophagesneutrophils |
| spellingShingle | Beata Drzewiecka Agata Przekora Dominika Dobko Aleksandra Kozera Katarzyna Krać Dominika Nguyen Ngoc Eric Fernández-De la Cruz Joanna Wessely-Szponder Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE) Materials antimicrobial peptides biomaterial blood derived products inflammation macrophages neutrophils |
| title | Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE) |
| title_full | Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE) |
| title_fullStr | Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE) |
| title_full_unstemmed | Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE) |
| title_short | Analysis of In Vitro Leukocyte Responses to Biomaterials in the Presence of Antimicrobial Porcine Neutrophil Extract (AMPNE) |
| title_sort | analysis of in vitro leukocyte responses to biomaterials in the presence of antimicrobial porcine neutrophil extract ampne |
| topic | antimicrobial peptides biomaterial blood derived products inflammation macrophages neutrophils |
| url | https://www.mdpi.com/1996-1944/16/16/5691 |
| work_keys_str_mv | AT beatadrzewiecka analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne AT agataprzekora analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne AT dominikadobko analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne AT aleksandrakozera analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne AT katarzynakrac analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne AT dominikanguyenngoc analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne AT ericfernandezdelacruz analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne AT joannawesselyszponder analysisofinvitroleukocyteresponsestobiomaterialsinthepresenceofantimicrobialporcineneutrophilextractampne |