Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop
It is currently difficult to perform accurate single-cell assays in 384-well plates. Here the authors report Dye Drop which uses sequential density displacement and microscopy for multi-step assays on cells, and use this to collect single-cell dose-response data for small molecules in breast cancer...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34536-7 |
| _version_ | 1798016866099134464 |
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| author | Caitlin E. Mills Kartik Subramanian Marc Hafner Mario Niepel Luca Gerosa Mirra Chung Chiara Victor Benjamin Gaudio Clarence Yapp Ajit J. Nirmal Nicholas Clark Peter K. Sorger |
| author_facet | Caitlin E. Mills Kartik Subramanian Marc Hafner Mario Niepel Luca Gerosa Mirra Chung Chiara Victor Benjamin Gaudio Clarence Yapp Ajit J. Nirmal Nicholas Clark Peter K. Sorger |
| author_sort | Caitlin E. Mills |
| collection | DOAJ |
| description | It is currently difficult to perform accurate single-cell assays in 384-well plates. Here the authors report Dye Drop which uses sequential density displacement and microscopy for multi-step assays on cells, and use this to collect single-cell dose-response data for small molecules in breast cancer cells. |
| first_indexed | 2024-04-11T15:57:29Z |
| format | Article |
| id | doaj.art-fd5702d7273c4618abdb7be6d446e8fd |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T15:57:29Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-fd5702d7273c4618abdb7be6d446e8fd2022-12-22T04:15:08ZengNature PortfolioNature Communications2041-17232022-11-0113111810.1038/s41467-022-34536-7Multiplexed and reproducible high content screening of live and fixed cells using Dye DropCaitlin E. Mills0Kartik Subramanian1Marc Hafner2Mario Niepel3Luca Gerosa4Mirra Chung5Chiara Victor6Benjamin Gaudio7Clarence Yapp8Ajit J. Nirmal9Nicholas Clark10Peter K. Sorger11Laboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolLaboratory of Systems Pharmacology, Department of Systems Biology, Harvard Medical SchoolIt is currently difficult to perform accurate single-cell assays in 384-well plates. Here the authors report Dye Drop which uses sequential density displacement and microscopy for multi-step assays on cells, and use this to collect single-cell dose-response data for small molecules in breast cancer cells.https://doi.org/10.1038/s41467-022-34536-7 |
| spellingShingle | Caitlin E. Mills Kartik Subramanian Marc Hafner Mario Niepel Luca Gerosa Mirra Chung Chiara Victor Benjamin Gaudio Clarence Yapp Ajit J. Nirmal Nicholas Clark Peter K. Sorger Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop Nature Communications |
| title | Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop |
| title_full | Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop |
| title_fullStr | Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop |
| title_full_unstemmed | Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop |
| title_short | Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop |
| title_sort | multiplexed and reproducible high content screening of live and fixed cells using dye drop |
| url | https://doi.org/10.1038/s41467-022-34536-7 |
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