The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced Photoaging
The objective of this research was to extract and prepare mycosporine-like amino acids (MAAs) and investigate the mechanism by which they act against UV-induced skin photoaging in Institute of Cancer Research (ICR ) mice. MAAs such as porphyra-334 and shinorine were extracted from <i>Porphyra...
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MDPI AG
2019-08-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/8/470 |
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| author | Rui Ying Zhaohui Zhang Huiying Zhu Bafang Li Hu Hou |
| author_facet | Rui Ying Zhaohui Zhang Huiying Zhu Bafang Li Hu Hou |
| author_sort | Rui Ying |
| collection | DOAJ |
| description | The objective of this research was to extract and prepare mycosporine-like amino acids (MAAs) and investigate the mechanism by which they act against UV-induced skin photoaging in Institute of Cancer Research (ICR ) mice. MAAs such as porphyra-334 and shinorine were extracted from <i>Porphyra yezoensis</i>, separated, and purified using column chromatography with SA-2 cation exchange resin. The effects of MAAs on the activity of endogenous antioxidant enzymes, namely total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA) were analyzed in mouse skin tissue. Pathological changes of skin tissue caused by ultraviolet radiation and the arrangement of collagen were observed by Hematoxylin-Eosin (HE) staining and scanning electron microscopy (SEM). Interleukin 1β (IL-1β), IL-6, and IL-10 were detected using the quantitative real-time reverse transcription-polymerase chain reaction (qPCR) and Enzyme Linked Immunosorbent Assay (ELISA). The concentration and expression of these proinflammatory cytokines was associated with the presence of nuclear factor (NF)-κB. The results show that MAA compounds from <i>Porphyra yezoensis</i> could suppress UV-induced photoaging of skin by inhibiting the reduction of endogenous antioxidant enzymes. Compared to the control group, the concentrations of SOD, GSH-Px, and CAT increased significantly in skin tissue homogenate following the external administration of MAAs (<i>p < 0.05, p < 0.01</i>), while the content of MDA decreased significantly (<i>p < 0.05</i>). Meanwhile, the administration of MAAs was associated with down-regulations in the concentration and mRNA expression of NF-κB, IL-1β, IL-6, and IL-10. The results suggest that MAAs could protect skin from photodamage by increasing antioxidant enzyme activities and inhibiting inflammation. |
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| last_indexed | 2024-04-14T02:24:58Z |
| publishDate | 2019-08-01 |
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| series | Marine Drugs |
| spelling | doaj.art-fd65aa9989fb4acf9063d1a93d38f62b2022-12-22T02:17:54ZengMDPI AGMarine Drugs1660-33972019-08-0117847010.3390/md17080470md17080470The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced PhotoagingRui Ying0Zhaohui Zhang1Huiying Zhu2Bafang Li3Hu Hou4College of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, No.5, Yu Shan Road, Qingdao 266003, ChinaThe objective of this research was to extract and prepare mycosporine-like amino acids (MAAs) and investigate the mechanism by which they act against UV-induced skin photoaging in Institute of Cancer Research (ICR ) mice. MAAs such as porphyra-334 and shinorine were extracted from <i>Porphyra yezoensis</i>, separated, and purified using column chromatography with SA-2 cation exchange resin. The effects of MAAs on the activity of endogenous antioxidant enzymes, namely total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and malondialdehyde (MDA) were analyzed in mouse skin tissue. Pathological changes of skin tissue caused by ultraviolet radiation and the arrangement of collagen were observed by Hematoxylin-Eosin (HE) staining and scanning electron microscopy (SEM). Interleukin 1β (IL-1β), IL-6, and IL-10 were detected using the quantitative real-time reverse transcription-polymerase chain reaction (qPCR) and Enzyme Linked Immunosorbent Assay (ELISA). The concentration and expression of these proinflammatory cytokines was associated with the presence of nuclear factor (NF)-κB. The results show that MAA compounds from <i>Porphyra yezoensis</i> could suppress UV-induced photoaging of skin by inhibiting the reduction of endogenous antioxidant enzymes. Compared to the control group, the concentrations of SOD, GSH-Px, and CAT increased significantly in skin tissue homogenate following the external administration of MAAs (<i>p < 0.05, p < 0.01</i>), while the content of MDA decreased significantly (<i>p < 0.05</i>). Meanwhile, the administration of MAAs was associated with down-regulations in the concentration and mRNA expression of NF-κB, IL-1β, IL-6, and IL-10. The results suggest that MAAs could protect skin from photodamage by increasing antioxidant enzyme activities and inhibiting inflammation.https://www.mdpi.com/1660-3397/17/8/470<i>Porphyra yezoensis</i>MAAsantioxidant enzymesinflammationNF-κB |
| spellingShingle | Rui Ying Zhaohui Zhang Huiying Zhu Bafang Li Hu Hou The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced Photoaging Marine Drugs <i>Porphyra yezoensis</i> MAAs antioxidant enzymes inflammation NF-κB |
| title | The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced Photoaging |
| title_full | The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced Photoaging |
| title_fullStr | The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced Photoaging |
| title_full_unstemmed | The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced Photoaging |
| title_short | The Protective Effect of Mycosporine-Like Amino Acids (MAAs) from <i>Porphyra yezoensi</i>s in a Mouse Model of UV Irradiation-Induced Photoaging |
| title_sort | protective effect of mycosporine like amino acids maas from i porphyra yezoensi i s in a mouse model of uv irradiation induced photoaging |
| topic | <i>Porphyra yezoensis</i> MAAs antioxidant enzymes inflammation NF-κB |
| url | https://www.mdpi.com/1660-3397/17/8/470 |
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