Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model
Abstract. Background:. Hair follicles are easily accessible and contain stem cells with different developmental origins, including mesenchymal stem cells (MSCs), that consequently reveal the potential of human hair follicle (hHF)-derived MSCs in repair and regeneration. However, the role of hHF-MSCs...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2023-05-01
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| Series: | Chinese Medical Journal |
| Online Access: | http://journals.lww.com/10.1097/CM9.0000000000002542 |
| _version_ | 1797816703028035584 |
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| author | Yingyu Ma Zhiwei Lin Xiaoyi Chen Xin Zhao Yi Sun Ji Wang Xiaozhou Mou Hai Zou Jinyang Chen Yanjie Yin |
| author_facet | Yingyu Ma Zhiwei Lin Xiaoyi Chen Xin Zhao Yi Sun Ji Wang Xiaozhou Mou Hai Zou Jinyang Chen Yanjie Yin |
| author_sort | Yingyu Ma |
| collection | DOAJ |
| description | Abstract. Background:. Hair follicles are easily accessible and contain stem cells with different developmental origins, including mesenchymal stem cells (MSCs), that consequently reveal the potential of human hair follicle (hHF)-derived MSCs in repair and regeneration. However, the role of hHF-MSCs in Achilles tendinopathy (AT) remains unclear. The present study investigated the effects of hHF-MSCs on Achilles tendon repair in rabbits.
Methods:. First, we extracted and characterized hHF-MSCs. Then, a rabbit tendinopathy model was constructed to analyze the ability of hHF-MSCs to promote repair in vivo. Anatomical observation and pathological and biomechanical analyses were performed to determine the effect of hHF-MSCs on AT, and quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemical staining were performed to explore the molecular mechanisms through which hHF-MSCs affects AT. Furthermore, statistical analyses were performed using independent sample t test, one-way analysis of variance (ANOVA), and one-way repeated measures multivariate ANOVA as appropriate.
Results:. Flow cytometry, a trilineage-induced differentiation test, confirmed that hHF-derived stem cells were derived from MSCs. The effect of hHF-MSCs on AT revealed that the Achilles tendon was anatomically healthy, as well as the maximum load carried by the Achilles tendon and hydroxyproline proteomic levels were increased. Moreover, collagen I and III were upregulated in rabbit AT treated with hHF-MSCs (compared with AT group; P < 0.05). Analysis of the molecular mechanisms revealed that hHF-MSCs promoted collagen fiber regeneration, possibly through Tenascin-C (TNC) upregulation and matrix metalloproteinase (MMP)-9 downregulation.
Conclusions:. hHF-MSCs can be a treatment modality to promote AT repair in rabbits by upregulating collagen I and III. Further analysis revealed that treatment of AT using hHF-MSCs promoted the regeneration of collagen fiber, possibly because of upregulation of TNC and downregulation of MMP-9, thus suggesting that hHF-MSCs are more promising for AT. |
| first_indexed | 2024-03-13T08:41:37Z |
| format | Article |
| id | doaj.art-fd664bf118cf407d9e024adfc869dc06 |
| institution | Directory Open Access Journal |
| issn | 0366-6999 2542-5641 |
| language | English |
| last_indexed | 2024-03-13T08:41:37Z |
| publishDate | 2023-05-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Chinese Medical Journal |
| spelling | doaj.art-fd664bf118cf407d9e024adfc869dc062023-05-30T09:28:14ZengWolters KluwerChinese Medical Journal0366-69992542-56412023-05-0113691089109710.1097/CM9.0000000000002542202305050-00011Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy modelYingyu MaZhiwei LinXiaoyi ChenXin ZhaoYi SunJi WangXiaozhou MouHai ZouJinyang ChenYanjie YinAbstract. Background:. Hair follicles are easily accessible and contain stem cells with different developmental origins, including mesenchymal stem cells (MSCs), that consequently reveal the potential of human hair follicle (hHF)-derived MSCs in repair and regeneration. However, the role of hHF-MSCs in Achilles tendinopathy (AT) remains unclear. The present study investigated the effects of hHF-MSCs on Achilles tendon repair in rabbits. Methods:. First, we extracted and characterized hHF-MSCs. Then, a rabbit tendinopathy model was constructed to analyze the ability of hHF-MSCs to promote repair in vivo. Anatomical observation and pathological and biomechanical analyses were performed to determine the effect of hHF-MSCs on AT, and quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemical staining were performed to explore the molecular mechanisms through which hHF-MSCs affects AT. Furthermore, statistical analyses were performed using independent sample t test, one-way analysis of variance (ANOVA), and one-way repeated measures multivariate ANOVA as appropriate. Results:. Flow cytometry, a trilineage-induced differentiation test, confirmed that hHF-derived stem cells were derived from MSCs. The effect of hHF-MSCs on AT revealed that the Achilles tendon was anatomically healthy, as well as the maximum load carried by the Achilles tendon and hydroxyproline proteomic levels were increased. Moreover, collagen I and III were upregulated in rabbit AT treated with hHF-MSCs (compared with AT group; P < 0.05). Analysis of the molecular mechanisms revealed that hHF-MSCs promoted collagen fiber regeneration, possibly through Tenascin-C (TNC) upregulation and matrix metalloproteinase (MMP)-9 downregulation. Conclusions:. hHF-MSCs can be a treatment modality to promote AT repair in rabbits by upregulating collagen I and III. Further analysis revealed that treatment of AT using hHF-MSCs promoted the regeneration of collagen fiber, possibly because of upregulation of TNC and downregulation of MMP-9, thus suggesting that hHF-MSCs are more promising for AT.http://journals.lww.com/10.1097/CM9.0000000000002542 |
| spellingShingle | Yingyu Ma Zhiwei Lin Xiaoyi Chen Xin Zhao Yi Sun Ji Wang Xiaozhou Mou Hai Zou Jinyang Chen Yanjie Yin Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model Chinese Medical Journal |
| title | Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model |
| title_full | Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model |
| title_fullStr | Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model |
| title_full_unstemmed | Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model |
| title_short | Human hair follicle-derived mesenchymal stem cells promote tendon repair in a rabbit Achilles tendinopathy model |
| title_sort | human hair follicle derived mesenchymal stem cells promote tendon repair in a rabbit achilles tendinopathy model |
| url | http://journals.lww.com/10.1097/CM9.0000000000002542 |
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