Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells
Objective To evaluate the effect of forkhead box protein B2 （FOXB2） on the proliferation, invasion and migration of hepatocellular carcinoma cells. Methods The expression levels of FOXB2 mRNA in normal liver cells LO2, hepatocellular carcinoma cells Hep3B and Huh7 were detected by quantitative PCR....
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of New Medicine
2022-01-01
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| Series: | Xin yixue |
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| Online Access: | https://www.xinyixue.cn/fileup/0253-9802/PDF/1644558872372-1994478869.pdf |
| _version_ | 1798016378597277696 |
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| author | Liang Junhui, Chen Zerui, Peng Shuming, Chen Shanjia, Shu Bairong |
| author_facet | Liang Junhui, Chen Zerui, Peng Shuming, Chen Shanjia, Shu Bairong |
| author_sort | Liang Junhui, Chen Zerui, Peng Shuming, Chen Shanjia, Shu Bairong |
| collection | DOAJ |
| description | Objective To evaluate the effect of forkhead box protein B2 （FOXB2） on the proliferation, invasion and migration of hepatocellular carcinoma cells. Methods The expression levels of FOXB2 mRNA in normal liver cells LO2, hepatocellular carcinoma cells Hep3B and Huh7 were detected by quantitative PCR. The cell model overexpressing FOXB2 was constructed. The overexpression efficiency was assessed by real-time fluorescent quantitative PCR and Western blot. The effect of FOXB2 on Huh7 cell proliferation was evaluated by cell counting kit-8 （CCK-8）. The effect of FOXB2 on the clone formation of Huh7 cells was determined by plate cloning assay. The effect of FOXB2 on the invasion and migration of Huh7 cells was detected by Transwell chamber test. Results The expression levels of FOXB2 in hepatocellular carcinoma cells Huh7 and Hep3B were significantly lower than that in normal liver cells LO2 （both P<0.001）. Overexpression of FOXB2 significantly inhibited the proliferation （P=0.005） and clone formation （P<0.001） of Huh7 cells. In the Huh7-FOXB2 overexpression group, the invasion （P=0.002） and migration capabilities （P<0.001） of hepatocellular carcinoma cells were significantly lower than that in the Vector control cells. Conclusions FOXB2 is lowly expressed in hepatocellular carcinoma cells. Overexpression of FOXB2 suppresses the proliferation, invasion and migration of hepatocellular carcinoma cells. |
| first_indexed | 2024-04-11T15:49:45Z |
| format | Article |
| id | doaj.art-fd677db6f5bc41b1a9ef51cf28cf3f60 |
| institution | Directory Open Access Journal |
| issn | 0253-9802 |
| language | zho |
| last_indexed | 2024-04-11T15:49:45Z |
| publishDate | 2022-01-01 |
| publisher | Editorial Office of Journal of New Medicine |
| record_format | Article |
| series | Xin yixue |
| spelling | doaj.art-fd677db6f5bc41b1a9ef51cf28cf3f602022-12-22T04:15:25ZzhoEditorial Office of Journal of New MedicineXin yixue0253-98022022-01-01531414510.3969/j.issn.0253-9802.2022.01.010Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cellsLiang Junhui, Chen Zerui, Peng Shuming, Chen Shanjia, Shu Bairong0△Department of General Surgery, Guangdong Provincial People’s Hospital Nanhai Hospital, Foshan 528200, ChinaObjective To evaluate the effect of forkhead box protein B2 （FOXB2） on the proliferation, invasion and migration of hepatocellular carcinoma cells. Methods The expression levels of FOXB2 mRNA in normal liver cells LO2, hepatocellular carcinoma cells Hep3B and Huh7 were detected by quantitative PCR. The cell model overexpressing FOXB2 was constructed. The overexpression efficiency was assessed by real-time fluorescent quantitative PCR and Western blot. The effect of FOXB2 on Huh7 cell proliferation was evaluated by cell counting kit-8 （CCK-8）. The effect of FOXB2 on the clone formation of Huh7 cells was determined by plate cloning assay. The effect of FOXB2 on the invasion and migration of Huh7 cells was detected by Transwell chamber test. Results The expression levels of FOXB2 in hepatocellular carcinoma cells Huh7 and Hep3B were significantly lower than that in normal liver cells LO2 （both P<0.001）. Overexpression of FOXB2 significantly inhibited the proliferation （P=0.005） and clone formation （P<0.001） of Huh7 cells. In the Huh7-FOXB2 overexpression group, the invasion （P=0.002） and migration capabilities （P<0.001） of hepatocellular carcinoma cells were significantly lower than that in the Vector control cells. Conclusions FOXB2 is lowly expressed in hepatocellular carcinoma cells. Overexpression of FOXB2 suppresses the proliferation, invasion and migration of hepatocellular carcinoma cells.https://www.xinyixue.cn/fileup/0253-9802/PDF/1644558872372-1994478869.pdf|hepatocellular carcinoma|forkhead box protein b2|proliferation|invasion|migration |
| spellingShingle | Liang Junhui, Chen Zerui, Peng Shuming, Chen Shanjia, Shu Bairong Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells Xin yixue |hepatocellular carcinoma|forkhead box protein b2|proliferation|invasion|migration |
| title | Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells |
| title_full | Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells |
| title_fullStr | Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells |
| title_full_unstemmed | Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells |
| title_short | Effect of overexpression of forkhead box B2 on proliferation, invasion and migration of hepatocellular carcinoma cells |
| title_sort | effect of overexpression of forkhead box b2 on proliferation invasion and migration of hepatocellular carcinoma cells |
| topic | |hepatocellular carcinoma|forkhead box protein b2|proliferation|invasion|migration |
| url | https://www.xinyixue.cn/fileup/0253-9802/PDF/1644558872372-1994478869.pdf |
| work_keys_str_mv | AT liangjunhuichenzeruipengshumingchenshanjiashubairong effectofoverexpressionofforkheadboxb2onproliferationinvasionandmigrationofhepatocellularcarcinomacells |