Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis
Hemp protein isolate (HPI) was used as raw material to modify HPI through ultra-high pressure assisted enzymatic hydrolysis reaction. The solubility and degree of hydrolysis were used as criteria to screen the optimal conditions for the enzymatic hydrolysis modification. The influence of ultra-high...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2023-10-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023010016 |
| _version_ | 1797661961472704512 |
|---|---|
| author | Rongxu LIU Chunyu LI Yucong WANG Zhixin XIE Yitong XIE Shuangpeng LI Danyi LIU Jianchun HAN |
| author_facet | Rongxu LIU Chunyu LI Yucong WANG Zhixin XIE Yitong XIE Shuangpeng LI Danyi LIU Jianchun HAN |
| author_sort | Rongxu LIU |
| collection | DOAJ |
| description | Hemp protein isolate (HPI) was used as raw material to modify HPI through ultra-high pressure assisted enzymatic hydrolysis reaction. The solubility and degree of hydrolysis were used as criteria to screen the optimal conditions for the enzymatic hydrolysis modification. The influence of ultra-high pressure assisted enzymatic hydrolysis on solubility, foaming, emulsification, water retention and oil retention of enzymatic hydrolysis products was explored. The results showed that the optimum conditions for HPI enzymatic reaction were as follows: Adding amount of enzyme (complex protease) 5000 U/g, pH8.0, temperature 55 ℃ and time 50 min. Taking HPI as the control, when the pressure was 200 MPa, the solubility, foaming property, emulsification property and oil holding capacity of enzymatic hydrolysis products were the highest, when the pressure was 100 MPa, the foaming stability was the best, the emulsification stability after enzymatic hydrolysis had different degrees of decline, when the pressure was 0.1 MPa, the water holding capacity reached the maximum. In conclusion, the ultrahigh pressure technology can effectively promote the enzymatic hydrolysis modification reaction of HPI, and the physical and chemical properties of the enzymatic hydrolysis products were the best when the pressure is 200 MPa. |
| first_indexed | 2024-03-11T18:52:14Z |
| format | Article |
| id | doaj.art-fd6b6d592381421aa8a30d5e8a4da1b9 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-11T18:52:14Z |
| publishDate | 2023-10-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-fd6b6d592381421aa8a30d5e8a4da1b92023-10-11T09:18:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-10-0144199910710.13386/j.issn1002-0306.20230100162023010016-19Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic HydrolysisRongxu LIU0Chunyu LI1Yucong WANG2Zhixin XIE3Yitong XIE4Shuangpeng LI5Danyi LIU6Jianchun HAN7Heilongjiang Green Food Science Research Institute, Harbin 150028, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Green Food Science Research Institute, Harbin 150028, ChinaCollege of Food Science, Northeast Agricultural University, Harbin 150030, ChinaHemp protein isolate (HPI) was used as raw material to modify HPI through ultra-high pressure assisted enzymatic hydrolysis reaction. The solubility and degree of hydrolysis were used as criteria to screen the optimal conditions for the enzymatic hydrolysis modification. The influence of ultra-high pressure assisted enzymatic hydrolysis on solubility, foaming, emulsification, water retention and oil retention of enzymatic hydrolysis products was explored. The results showed that the optimum conditions for HPI enzymatic reaction were as follows: Adding amount of enzyme (complex protease) 5000 U/g, pH8.0, temperature 55 ℃ and time 50 min. Taking HPI as the control, when the pressure was 200 MPa, the solubility, foaming property, emulsification property and oil holding capacity of enzymatic hydrolysis products were the highest, when the pressure was 100 MPa, the foaming stability was the best, the emulsification stability after enzymatic hydrolysis had different degrees of decline, when the pressure was 0.1 MPa, the water holding capacity reached the maximum. In conclusion, the ultrahigh pressure technology can effectively promote the enzymatic hydrolysis modification reaction of HPI, and the physical and chemical properties of the enzymatic hydrolysis products were the best when the pressure is 200 MPa.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023010016hemp protein isolate (hpi)ultra-high pressure processing (uhp)enzymatic hydrolysisphysicochemical property |
| spellingShingle | Rongxu LIU Chunyu LI Yucong WANG Zhixin XIE Yitong XIE Shuangpeng LI Danyi LIU Jianchun HAN Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis Shipin gongye ke-ji hemp protein isolate (hpi) ultra-high pressure processing (uhp) enzymatic hydrolysis physicochemical property |
| title | Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis |
| title_full | Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis |
| title_fullStr | Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis |
| title_full_unstemmed | Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis |
| title_short | Study on the Modification and Physicochemical Properties of Hemp Protein Isolate by Ultra-High Pressure Assisted Enzymatic Hydrolysis |
| title_sort | study on the modification and physicochemical properties of hemp protein isolate by ultra high pressure assisted enzymatic hydrolysis |
| topic | hemp protein isolate (hpi) ultra-high pressure processing (uhp) enzymatic hydrolysis physicochemical property |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023010016 |
| work_keys_str_mv | AT rongxuliu studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis AT chunyuli studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis AT yucongwang studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis AT zhixinxie studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis AT yitongxie studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis AT shuangpengli studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis AT danyiliu studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis AT jianchunhan studyonthemodificationandphysicochemicalpropertiesofhempproteinisolatebyultrahighpressureassistedenzymatichydrolysis |