The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis
Chronic rhinosinusitis (CRS) is marked by an inflamed mucosa of sinuses and is accompanied by a significantly reduced quality of live. Since no guidelines for the treatment of CRS are available, long lasting clinical histories with health care costs adding up to dozens of billion $ annually are caus...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02325/full |
| _version_ | 1818554976609239040 |
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| author | Matthias Schürmann Felix Oppel Martin Gottschalk Björn Büker Christian Andreas Jantos Cornelius Knabbe Andreas Hütten Barbara Kaltschmidt Christian Kaltschmidt Holger Sudhoff |
| author_facet | Matthias Schürmann Felix Oppel Martin Gottschalk Björn Büker Christian Andreas Jantos Cornelius Knabbe Andreas Hütten Barbara Kaltschmidt Christian Kaltschmidt Holger Sudhoff |
| author_sort | Matthias Schürmann |
| collection | DOAJ |
| description | Chronic rhinosinusitis (CRS) is marked by an inflamed mucosa of sinuses and is accompanied by a significantly reduced quality of live. Since no guidelines for the treatment of CRS are available, long lasting clinical histories with health care costs adding up to dozens of billion $ annually are caused by CRS. The progression of CRS is often induced by bacterial infections and/or a shift in microbiome as well as biofilm formation. The exact microbiome alterations are still unclear and the impenetrable biofilm renders the treatment with common antibiotics ineffective. This study focuses on characterizing the microbiome changes in CRS and investigating the inhibition of biofilm growth by 1,8-Cineol, a small, non-polar and hence biofilm penetrating molecule with known antimicrobial potential. We performed MALDI-TOF MS based characterization of the microbiomes of healthy individuals and CRS patients (n = 50). The microbiome in our test group was shifted to pathogens (Staphylococcus aureus, Escherichia coli, and Moraxella catarrhalis). In contrast to published studies, solely based on cell culture techniques, we could not verify the abundance of Pseudomonas aeruginosa in CRS. The inhibition of bacterial proliferation and biofilm growth by 1,8-Cineol was measured for these three pathogens. Interestingly, S. aureus, the most prominent germ in CRS, showed a biofilm inhibition not simply correlated to its inhibition of proliferation. RT-qPCR confirmed that this was due to the downregulations of major key players in biofilm generation (agrA, SarA and σB) by 1,8-Cineol. Furthermore we verified this high biofilm inhibition potential in a model host system consisting out of S. aureus biofilm grown on mature respiratory epithelium. A second host model, comprising organotypic slices, was utilized to investigate the reaction of the innate immune system present in the nasal mucosa upon biofilm formation and treatment with 1,8-Cineol. Interestingly Staphylococcus epidermidis, the cause of very common catheter infections, possesses a biofilm generation pathway very similar to S. aureus and might be treatable in a similar fashion. The two presented in vitro model systems might be transferred to combinations of every biofilm forming bacterial with most kind of epithelium and mucosa. |
| first_indexed | 2024-12-12T09:47:11Z |
| format | Article |
| id | doaj.art-fd6bab5c326c42e99e3ed28f03f90ec2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T09:47:11Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-fd6bab5c326c42e99e3ed28f03f90ec22022-12-22T00:28:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-10-011010.3389/fmicb.2019.02325466557The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic RhinosinusitisMatthias Schürmann0Felix Oppel1Martin Gottschalk2Björn Büker3Christian Andreas Jantos4Cornelius Knabbe5Andreas Hütten6Barbara Kaltschmidt7Christian Kaltschmidt8Holger Sudhoff9Department of Otolaryngology, Head and Neck Surgery, Klinikum Bielefeld, Bielefeld, GermanyDepartment of Otolaryngology, Head and Neck Surgery, Klinikum Bielefeld, Bielefeld, GermanyThin Films and Physics of Nanostructures, Faculty of Physics, Bielefeld University, Bielefeld, GermanyThin Films and Physics of Nanostructures, Faculty of Physics, Bielefeld University, Bielefeld, GermanyInstitute of Laboratory Medicine and Microbiology, Bielefeld, GermanyInstitute for Laboratory and Transfusion Medicine, Heart and Diabetes Center North Rhine-Westphalia, Bad Oeynhausen, GermanyThin Films and Physics of Nanostructures, Faculty of Physics, Bielefeld University, Bielefeld, GermanyMolecular Neurobiology, Faculty of Biology, Bielefeld University, Bielefeld, GermanyDepartment of Cell Biology, Faculty of Biology, Bielefeld University, Bielefeld, GermanyDepartment of Otolaryngology, Head and Neck Surgery, Klinikum Bielefeld, Bielefeld, GermanyChronic rhinosinusitis (CRS) is marked by an inflamed mucosa of sinuses and is accompanied by a significantly reduced quality of live. Since no guidelines for the treatment of CRS are available, long lasting clinical histories with health care costs adding up to dozens of billion $ annually are caused by CRS. The progression of CRS is often induced by bacterial infections and/or a shift in microbiome as well as biofilm formation. The exact microbiome alterations are still unclear and the impenetrable biofilm renders the treatment with common antibiotics ineffective. This study focuses on characterizing the microbiome changes in CRS and investigating the inhibition of biofilm growth by 1,8-Cineol, a small, non-polar and hence biofilm penetrating molecule with known antimicrobial potential. We performed MALDI-TOF MS based characterization of the microbiomes of healthy individuals and CRS patients (n = 50). The microbiome in our test group was shifted to pathogens (Staphylococcus aureus, Escherichia coli, and Moraxella catarrhalis). In contrast to published studies, solely based on cell culture techniques, we could not verify the abundance of Pseudomonas aeruginosa in CRS. The inhibition of bacterial proliferation and biofilm growth by 1,8-Cineol was measured for these three pathogens. Interestingly, S. aureus, the most prominent germ in CRS, showed a biofilm inhibition not simply correlated to its inhibition of proliferation. RT-qPCR confirmed that this was due to the downregulations of major key players in biofilm generation (agrA, SarA and σB) by 1,8-Cineol. Furthermore we verified this high biofilm inhibition potential in a model host system consisting out of S. aureus biofilm grown on mature respiratory epithelium. A second host model, comprising organotypic slices, was utilized to investigate the reaction of the innate immune system present in the nasal mucosa upon biofilm formation and treatment with 1,8-Cineol. Interestingly Staphylococcus epidermidis, the cause of very common catheter infections, possesses a biofilm generation pathway very similar to S. aureus and might be treatable in a similar fashion. The two presented in vitro model systems might be transferred to combinations of every biofilm forming bacterial with most kind of epithelium and mucosa.https://www.frontiersin.org/article/10.3389/fmicb.2019.02325/fullbiofilmchronic rhinosinusitisS. aureushost model system18-Cineol |
| spellingShingle | Matthias Schürmann Felix Oppel Martin Gottschalk Björn Büker Christian Andreas Jantos Cornelius Knabbe Andreas Hütten Barbara Kaltschmidt Christian Kaltschmidt Holger Sudhoff The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis Frontiers in Microbiology biofilm chronic rhinosinusitis S. aureus host model system 1 8-Cineol |
| title | The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis |
| title_full | The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis |
| title_fullStr | The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis |
| title_full_unstemmed | The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis |
| title_short | The Therapeutic Effect of 1,8-Cineol on Pathogenic Bacteria Species Present in Chronic Rhinosinusitis |
| title_sort | therapeutic effect of 1 8 cineol on pathogenic bacteria species present in chronic rhinosinusitis |
| topic | biofilm chronic rhinosinusitis S. aureus host model system 1 8-Cineol |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02325/full |
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