Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers
Arsenic exposure alters redox balance, induces DNA damage, and deregulates many genes. OGG1 gene involved in base repair mechanism, for excision of 8-oxoguanine (8-oxoG) from DNA formed as a result of accumulation of ROS in cell. HPRT gene encode transferase enzymes involved in purine recycling mech...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524634/?tool=EBI |
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| author | Zertashia Akram Ishrat Mahjabeen Muhammad Umair Muhammad Fahim Mahmood Akhter Kayani Lulu Fatima Malik Waqar Ahmad Sarwat Jahan Tayyaba Afsar Ali Almajwal Nawaf W. Alruwaili Iftikhar Ali Khawar Suhail Razak |
| author_facet | Zertashia Akram Ishrat Mahjabeen Muhammad Umair Muhammad Fahim Mahmood Akhter Kayani Lulu Fatima Malik Waqar Ahmad Sarwat Jahan Tayyaba Afsar Ali Almajwal Nawaf W. Alruwaili Iftikhar Ali Khawar Suhail Razak |
| author_sort | Zertashia Akram |
| collection | DOAJ |
| description | Arsenic exposure alters redox balance, induces DNA damage, and deregulates many genes. OGG1 gene involved in base repair mechanism, for excision of 8-oxoguanine (8-oxoG) from DNA formed as a result of accumulation of ROS in cell. HPRT gene encode transferase enzymes involved in purine recycling mechanism. The main focus of the study was to evaluate the expression variation in HPRT, OGG1 gene expression, and DNA damage of industrial workers. Blood samples of 300 occupational workers were collected from welding, brick kiln, furniture, pesticide, and paint industry (n = 60/industry) to evaluate the expression variation in HPRT, OGG1 gene expression, and DNA damage in blood cells by comet assay along with age and gender matched 300 control individuals. Blood arsenic content was higher (P<0.001) in an industrial group compared to the control. OGG1 and HPRT expression were (P<0.05) downregulated in exposed workers compared to controls. Spearman correlation analysis showed a significant positive correlation between HPRT vs OGG1 (P< 0.0001) in exposed workers compared to controls. Altered expression of both genes was observed between workers with <25years and >25years of age as well as between workers with <10years and >10year exposure. Reduced expression (P<0.05) of both genes and a high extent of DNA damage was evident in exposed smokers compared to respective non-smokers. DNA fragmentation was higher (P<0.05) in the furniture, welding and brick kiln group compared to control, and other industries. The present study suggests that altered expression of OGG1 and HPRT gene induce oxidative stress, showed a negative impact on the recycling of purines leading to DNA damage which increase the vulnerability of workers to carcinogenicity. |
| first_indexed | 2024-04-12T03:25:41Z |
| format | Article |
| id | doaj.art-fd6bac21042642159b9ce30768210006 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:25:41Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fd6bac21042642159b9ce307682100062022-12-22T03:49:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workersZertashia AkramIshrat MahjabeenMuhammad UmairMuhammad FahimMahmood Akhter KayaniLulu FatimaMalik Waqar AhmadSarwat JahanTayyaba AfsarAli AlmajwalNawaf W. AlruwailiIftikhar Ali KhawarSuhail RazakArsenic exposure alters redox balance, induces DNA damage, and deregulates many genes. OGG1 gene involved in base repair mechanism, for excision of 8-oxoguanine (8-oxoG) from DNA formed as a result of accumulation of ROS in cell. HPRT gene encode transferase enzymes involved in purine recycling mechanism. The main focus of the study was to evaluate the expression variation in HPRT, OGG1 gene expression, and DNA damage of industrial workers. Blood samples of 300 occupational workers were collected from welding, brick kiln, furniture, pesticide, and paint industry (n = 60/industry) to evaluate the expression variation in HPRT, OGG1 gene expression, and DNA damage in blood cells by comet assay along with age and gender matched 300 control individuals. Blood arsenic content was higher (P<0.001) in an industrial group compared to the control. OGG1 and HPRT expression were (P<0.05) downregulated in exposed workers compared to controls. Spearman correlation analysis showed a significant positive correlation between HPRT vs OGG1 (P< 0.0001) in exposed workers compared to controls. Altered expression of both genes was observed between workers with <25years and >25years of age as well as between workers with <10years and >10year exposure. Reduced expression (P<0.05) of both genes and a high extent of DNA damage was evident in exposed smokers compared to respective non-smokers. DNA fragmentation was higher (P<0.05) in the furniture, welding and brick kiln group compared to control, and other industries. The present study suggests that altered expression of OGG1 and HPRT gene induce oxidative stress, showed a negative impact on the recycling of purines leading to DNA damage which increase the vulnerability of workers to carcinogenicity.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524634/?tool=EBI |
| spellingShingle | Zertashia Akram Ishrat Mahjabeen Muhammad Umair Muhammad Fahim Mahmood Akhter Kayani Lulu Fatima Malik Waqar Ahmad Sarwat Jahan Tayyaba Afsar Ali Almajwal Nawaf W. Alruwaili Iftikhar Ali Khawar Suhail Razak Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers PLoS ONE |
| title | Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers |
| title_full | Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers |
| title_fullStr | Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers |
| title_full_unstemmed | Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers |
| title_short | Expression variation of OGG1 and HPRT gene and DNA damage in arsenic exposed industrial workers |
| title_sort | expression variation of ogg1 and hprt gene and dna damage in arsenic exposed industrial workers |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524634/?tool=EBI |
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