<i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional Metabolites
Multifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be u...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-06-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/6/1280 |
| _version_ | 1797530433729069056 |
|---|---|
| author | Dana Byrtusová Martin Szotkowski Klára Kurowska Volha Shapaval Ivana Márová |
| author_facet | Dana Byrtusová Martin Szotkowski Klára Kurowska Volha Shapaval Ivana Márová |
| author_sort | Dana Byrtusová |
| collection | DOAJ |
| description | Multifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be used in the food and feed industry and the pharmaceutical industry, as well as a source of biofuels. In the study, we examined the effect of different nitrogen sources, carbon sources and CN ratios on the co-production of intracellular lipids, carotenoids, β–glucans and extracellular glycolipids. Yeast strain <i>R. kratochvilovae</i> CCY 20-2-26 was identified as the best co-producer of lipids (66.7 ± 1.5% of DCW), exoglycolipids (2.42 ± 0.08 g/L), β-glucan (11.33 ± 1.34% of DCW) and carotenoids (1.35 ± 0.11 mg/g), with a biomass content of 15.2 ± 0.8 g/L, by using the synthetic medium with potassium nitrate and mannose as a carbon source. It was shown that an increased C/N ratio positively affected the biomass yield and production of lipids and β-glucans. |
| first_indexed | 2024-03-10T10:28:53Z |
| format | Article |
| id | doaj.art-fd6d211daf4c408180b2ddc94cf445b0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:28:53Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fd6d211daf4c408180b2ddc94cf445b02023-11-21T23:49:27ZengMDPI AGMicroorganisms2076-26072021-06-0196128010.3390/microorganisms9061280<i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional MetabolitesDana Byrtusová0Martin Szotkowski1Klára Kurowska2Volha Shapaval3Ivana Márová4Faculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayFaculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech RepublicFaculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayFaculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 612 00 Brno, Czech RepublicMultifunctional biomass is able to provide more than one valuable product, and thus, it is attractive in the field of microbial biotechnology due to its economic feasibility. Carotenogenic yeasts are effective microbial factories for the biosynthesis of a broad spectrum of biomolecules that can be used in the food and feed industry and the pharmaceutical industry, as well as a source of biofuels. In the study, we examined the effect of different nitrogen sources, carbon sources and CN ratios on the co-production of intracellular lipids, carotenoids, β–glucans and extracellular glycolipids. Yeast strain <i>R. kratochvilovae</i> CCY 20-2-26 was identified as the best co-producer of lipids (66.7 ± 1.5% of DCW), exoglycolipids (2.42 ± 0.08 g/L), β-glucan (11.33 ± 1.34% of DCW) and carotenoids (1.35 ± 0.11 mg/g), with a biomass content of 15.2 ± 0.8 g/L, by using the synthetic medium with potassium nitrate and mannose as a carbon source. It was shown that an increased C/N ratio positively affected the biomass yield and production of lipids and β-glucans.https://www.mdpi.com/2076-2607/9/6/1280<i>Rhodotorula kratochvilovae</i>lipidsextracellular glycolipidscarotenoidsβ-glucan |
| spellingShingle | Dana Byrtusová Martin Szotkowski Klára Kurowska Volha Shapaval Ivana Márová <i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional Metabolites Microorganisms <i>Rhodotorula kratochvilovae</i> lipids extracellular glycolipids carotenoids β-glucan |
| title | <i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional Metabolites |
| title_full | <i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional Metabolites |
| title_fullStr | <i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional Metabolites |
| title_full_unstemmed | <i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional Metabolites |
| title_short | <i>Rhodotorula kratochvilovae</i> CCY 20-2-26—The Source of Multifunctional Metabolites |
| title_sort | i rhodotorula kratochvilovae i ccy 20 2 26 the source of multifunctional metabolites |
| topic | <i>Rhodotorula kratochvilovae</i> lipids extracellular glycolipids carotenoids β-glucan |
| url | https://www.mdpi.com/2076-2607/9/6/1280 |
| work_keys_str_mv | AT danabyrtusova irhodotorulakratochvilovaeiccy20226thesourceofmultifunctionalmetabolites AT martinszotkowski irhodotorulakratochvilovaeiccy20226thesourceofmultifunctionalmetabolites AT klarakurowska irhodotorulakratochvilovaeiccy20226thesourceofmultifunctionalmetabolites AT volhashapaval irhodotorulakratochvilovaeiccy20226thesourceofmultifunctionalmetabolites AT ivanamarova irhodotorulakratochvilovaeiccy20226thesourceofmultifunctionalmetabolites |