The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy
The water-soluble blue–green pigment marennine, produced and partly excreted by the diatom <i>Haslea ostrearia</i>, and known for a long time for its role in the greening of oysters, was isolated from the culture medium, purified, and analyzed by Nuclear Magnetic Resonance (NMR) in order...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/1/42 |
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| author | Ilhem Zebiri Boris Jacquette Nellie Francezon Mickaël Herbaut Amina Latigui Sullivan Bricaud Réjean Tremblay Pamela Pasetto Jean-Luc Mouget Jens Dittmer |
| author_facet | Ilhem Zebiri Boris Jacquette Nellie Francezon Mickaël Herbaut Amina Latigui Sullivan Bricaud Réjean Tremblay Pamela Pasetto Jean-Luc Mouget Jens Dittmer |
| author_sort | Ilhem Zebiri |
| collection | DOAJ |
| description | The water-soluble blue–green pigment marennine, produced and partly excreted by the diatom <i>Haslea ostrearia</i>, and known for a long time for its role in the greening of oysters, was isolated from the culture medium, purified, and analyzed by Nuclear Magnetic Resonance (NMR) in order to gain insight into its chemical structure. The spectra show mainly carbohydrates of a complex composition, apparently highly branched, and with a mass in the order of 10 kDa. There are, in addition, some signals of aliphatic and, much weaker, aromatic groups that present aglycons. The latter might be responsible for the color. These carbohydrates are always associated with the blue–green color and cannot be separated from it by most treatments; they are interpreted as constituting the frame of the pigment. NMR after hydrolysis identifies the most abundant monosaccharides in marennine as galactose, xylose, mannose, rhamnose, and fucose. |
| first_indexed | 2024-03-09T11:53:03Z |
| format | Article |
| id | doaj.art-fd6f608456ef4830a270ce0cfff4a48c |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T11:53:03Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-fd6f608456ef4830a270ce0cfff4a48c2023-11-30T23:13:43ZengMDPI AGMarine Drugs1660-33972023-01-012114210.3390/md21010042The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR SpectroscopyIlhem Zebiri0Boris Jacquette1Nellie Francezon2Mickaël Herbaut3Amina Latigui4Sullivan Bricaud5Réjean Tremblay6Pamela Pasetto7Jean-Luc Mouget8Jens Dittmer9Laboratoire Biologie des Organismes, Stress, Santé, Environnement (BiOSSE), Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceInstitut des Molécules et Matériaux du Mans (IMMM), UMR CNRS 6283, Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceLaboratoire Biologie des Organismes, Stress, Santé, Environnement (BiOSSE), Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceLaboratoire Biologie des Organismes, Stress, Santé, Environnement (BiOSSE), Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceInstitut des Molécules et Matériaux du Mans (IMMM), UMR CNRS 6283, Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceInstitut des Molécules et Matériaux du Mans (IMMM), UMR CNRS 6283, Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceInstitut des Sciences de la Mer, Université du Québec à Rimouski, 310 Allée des Ursulines, Rimouski, QC G5L 3A1, CanadaInstitut des Molécules et Matériaux du Mans (IMMM), UMR CNRS 6283, Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceLaboratoire Biologie des Organismes, Stress, Santé, Environnement (BiOSSE), Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceInstitut des Molécules et Matériaux du Mans (IMMM), UMR CNRS 6283, Le Mans Université, Av. Olivier Messiaen, 72085 Le Mans, FranceThe water-soluble blue–green pigment marennine, produced and partly excreted by the diatom <i>Haslea ostrearia</i>, and known for a long time for its role in the greening of oysters, was isolated from the culture medium, purified, and analyzed by Nuclear Magnetic Resonance (NMR) in order to gain insight into its chemical structure. The spectra show mainly carbohydrates of a complex composition, apparently highly branched, and with a mass in the order of 10 kDa. There are, in addition, some signals of aliphatic and, much weaker, aromatic groups that present aglycons. The latter might be responsible for the color. These carbohydrates are always associated with the blue–green color and cannot be separated from it by most treatments; they are interpreted as constituting the frame of the pigment. NMR after hydrolysis identifies the most abundant monosaccharides in marennine as galactose, xylose, mannose, rhamnose, and fucose.https://www.mdpi.com/1660-3397/21/1/42marennine<i>Haslea ostrearia</i>microalgaediatomspolysaccharidesblue pigment |
| spellingShingle | Ilhem Zebiri Boris Jacquette Nellie Francezon Mickaël Herbaut Amina Latigui Sullivan Bricaud Réjean Tremblay Pamela Pasetto Jean-Luc Mouget Jens Dittmer The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy Marine Drugs marennine <i>Haslea ostrearia</i> microalgae diatoms polysaccharides blue pigment |
| title | The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy |
| title_full | The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy |
| title_fullStr | The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy |
| title_full_unstemmed | The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy |
| title_short | The Polysaccharidic Nature of the Skeleton of Marennine as Determined by NMR Spectroscopy |
| title_sort | polysaccharidic nature of the skeleton of marennine as determined by nmr spectroscopy |
| topic | marennine <i>Haslea ostrearia</i> microalgae diatoms polysaccharides blue pigment |
| url | https://www.mdpi.com/1660-3397/21/1/42 |
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