Identification of olive (Olea europaea L.) cultivars by molecular markers
RAPD markers were applied to assess molecular polymorphisms within olive cultivars held in a collection orchard and to develop a system of a minimum number of primers for cultivar identification. Random Amplified Polymorphic DNA of fifteen cultivars ('Istrska belica', 'Štorta',...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2001-05-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/15674 |
| _version_ | 1797386952546189312 |
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| author | Dunja BANDELJ Jernej JAKŠE Branka JAVORNIK |
| author_facet | Dunja BANDELJ Jernej JAKŠE Branka JAVORNIK |
| author_sort | Dunja BANDELJ |
| collection | DOAJ |
| description |
RAPD markers were applied to assess molecular polymorphisms within olive cultivars held in a collection orchard and to develop a system of a minimum number of primers for cultivar identification. Random Amplified Polymorphic DNA of fifteen cultivars ('Istrska belica', 'Štorta', 'Buga', 'Črnica', ‘Unknown 2’, 'Leccino', 'Nocelara del belice', 'Frantoio', 'Cipressino', 'Santa Caterina', 'Pendolino', 'Arbequina', 'Maurino', 'Picholine', 'Unknown 1') was analysed, using twelve different arbitrary primers: OPA-1, OPA-4, OPA-8, OPX-2, OPX-3, OPX-4, OPX-8, OPX-11, OPX-12, OPX-13, OPX-14, OPX-19. The results confirmed identity within trees of thirteen cultivars in the collection orchard while mislabeling was found in two cultivars. The high polymorphism detected by RAPD markers was successfully used for the development of an identification key for rapid olive cultivar determination.
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| first_indexed | 2024-03-08T22:16:52Z |
| format | Article |
| id | doaj.art-fd71c72b6655452282fc2cb9ce2ed28b |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2024-03-08T22:16:52Z |
| publishDate | 2001-05-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-fd71c72b6655452282fc2cb9ce2ed28b2023-12-18T22:15:03ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412001-05-01771Identification of olive (Olea europaea L.) cultivars by molecular markersDunja BANDELJJernej JAKŠEBranka JAVORNIK0University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana RAPD markers were applied to assess molecular polymorphisms within olive cultivars held in a collection orchard and to develop a system of a minimum number of primers for cultivar identification. Random Amplified Polymorphic DNA of fifteen cultivars ('Istrska belica', 'Štorta', 'Buga', 'Črnica', ‘Unknown 2’, 'Leccino', 'Nocelara del belice', 'Frantoio', 'Cipressino', 'Santa Caterina', 'Pendolino', 'Arbequina', 'Maurino', 'Picholine', 'Unknown 1') was analysed, using twelve different arbitrary primers: OPA-1, OPA-4, OPA-8, OPX-2, OPX-3, OPX-4, OPX-8, OPX-11, OPX-12, OPX-13, OPX-14, OPX-19. The results confirmed identity within trees of thirteen cultivars in the collection orchard while mislabeling was found in two cultivars. The high polymorphism detected by RAPD markers was successfully used for the development of an identification key for rapid olive cultivar determination. https://journals.uni-lj.si/aas/article/view/15674identificationRAPDOlea europaeaSloveniaSlovene Istra |
| spellingShingle | Dunja BANDELJ Jernej JAKŠE Branka JAVORNIK Identification of olive (Olea europaea L.) cultivars by molecular markers Acta Agriculturae Slovenica identification RAPD Olea europaea Slovenia Slovene Istra |
| title | Identification of olive (Olea europaea L.) cultivars by molecular markers |
| title_full | Identification of olive (Olea europaea L.) cultivars by molecular markers |
| title_fullStr | Identification of olive (Olea europaea L.) cultivars by molecular markers |
| title_full_unstemmed | Identification of olive (Olea europaea L.) cultivars by molecular markers |
| title_short | Identification of olive (Olea europaea L.) cultivars by molecular markers |
| title_sort | identification of olive olea europaea l cultivars by molecular markers |
| topic | identification RAPD Olea europaea Slovenia Slovene Istra |
| url | https://journals.uni-lj.si/aas/article/view/15674 |
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