The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V Alloy
To chemically functionalize the Ti6Al4V alloy surface, a custom-made low-temperature atmospheric pressure plasma reactor device was used to polymerize heptylamine on it. The effect of different deposition times, an important process parameter, was also investigated. For each deposition time group, t...
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MDPI AG
2023-09-01
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| Online Access: | https://www.mdpi.com/1996-1944/16/19/6450 |
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| author | Di Le Jie Pan Haixia Xing |
| author_facet | Di Le Jie Pan Haixia Xing |
| author_sort | Di Le |
| collection | DOAJ |
| description | To chemically functionalize the Ti6Al4V alloy surface, a custom-made low-temperature atmospheric pressure plasma reactor device was used to polymerize heptylamine on it. The effect of different deposition times, an important process parameter, was also investigated. For each deposition time group, the surface morphology was observed via scanning electron microscopy (SEM). The surface chemical content was analyzed via X-ray photoelectron spectroscopy, and surface hydrophilicity was measured via water contact angle. The adhesion of bone marrow stromal cells (BMSCs) on the modified Ti6Al4V alloy surfaces was also observed via SEM. A quantitative evaluation of cell proliferation was performed via the Cell Counting Kit-8 assay. The results revealed that amino groups were introduced on the Ti6Al4V alloy surface via plasma-polymerized heptylamine (PPHA). The percentages of NH<sub>2</sub>/C for various deposition times (0 s, 30 s, 45 s, 60 s, 90 s, and 120 s) were 3.39%, 5.14%, 6.71%, 6.72%, 7.31%, and 7.65%. A 30 s, 45 s, and 60 s deposition time could significantly increase surface hydrophilicity with a mean water contact angle of 62.1 ± 1.6°, 65.7 ± 1.1°, and 88.2 ± 1.4°, respectively. Meanwhile, a 60 s, 90 s, and 120 s deposition time promoted BMSCs cell adhesion and proliferation. However, this promotion effect differed non-significantly among the three groups. In conclusion, the introduction of amino groups on the Ti6Al4V alloy surface exhibited surface modification and enhancement of cell adhesion and proliferation, which was partially associated with deposition time. |
| first_indexed | 2024-03-10T21:41:18Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T21:41:18Z |
| publishDate | 2023-09-01 |
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| spelling | doaj.art-fd759ff1baf549faa20756c2fbc58d6c2023-11-19T14:40:03ZengMDPI AGMaterials1996-19442023-09-011619645010.3390/ma16196450The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V AlloyDi Le0Jie Pan1Haixia Xing2Department of General Dentistry, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, ChinaDepartment of General Dentistry, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, ChinaDepartment of General Dentistry, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, ChinaTo chemically functionalize the Ti6Al4V alloy surface, a custom-made low-temperature atmospheric pressure plasma reactor device was used to polymerize heptylamine on it. The effect of different deposition times, an important process parameter, was also investigated. For each deposition time group, the surface morphology was observed via scanning electron microscopy (SEM). The surface chemical content was analyzed via X-ray photoelectron spectroscopy, and surface hydrophilicity was measured via water contact angle. The adhesion of bone marrow stromal cells (BMSCs) on the modified Ti6Al4V alloy surfaces was also observed via SEM. A quantitative evaluation of cell proliferation was performed via the Cell Counting Kit-8 assay. The results revealed that amino groups were introduced on the Ti6Al4V alloy surface via plasma-polymerized heptylamine (PPHA). The percentages of NH<sub>2</sub>/C for various deposition times (0 s, 30 s, 45 s, 60 s, 90 s, and 120 s) were 3.39%, 5.14%, 6.71%, 6.72%, 7.31%, and 7.65%. A 30 s, 45 s, and 60 s deposition time could significantly increase surface hydrophilicity with a mean water contact angle of 62.1 ± 1.6°, 65.7 ± 1.1°, and 88.2 ± 1.4°, respectively. Meanwhile, a 60 s, 90 s, and 120 s deposition time promoted BMSCs cell adhesion and proliferation. However, this promotion effect differed non-significantly among the three groups. In conclusion, the introduction of amino groups on the Ti6Al4V alloy surface exhibited surface modification and enhancement of cell adhesion and proliferation, which was partially associated with deposition time.https://www.mdpi.com/1996-1944/16/19/6450surface modificationheptylamineplasma polymerspolymerizationtitanium alloyTi6Al4V |
| spellingShingle | Di Le Jie Pan Haixia Xing The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V Alloy Materials surface modification heptylamine plasma polymers polymerization titanium alloy Ti6Al4V |
| title | The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V Alloy |
| title_full | The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V Alloy |
| title_fullStr | The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V Alloy |
| title_full_unstemmed | The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V Alloy |
| title_short | The Cell Adhesion and Proliferation Enhancement Impact of Low-Temperature Atmospheric Pressure Plasma-Polymerized Heptylamine on the Surface of Ti6Al4V Alloy |
| title_sort | cell adhesion and proliferation enhancement impact of low temperature atmospheric pressure plasma polymerized heptylamine on the surface of ti6al4v alloy |
| topic | surface modification heptylamine plasma polymers polymerization titanium alloy Ti6Al4V |
| url | https://www.mdpi.com/1996-1944/16/19/6450 |
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