Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary
Objectives Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting cats worldwide. The objectives of the study were to estimate the prevalence of these retroviruses in domestic cats in Hungary and to characterise the phylogenetic relationships of FIV strains....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2019-12-01
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| Series: | Journal of Feline Medicine and Surgery Open Reports |
| Online Access: | https://doi.org/10.1177/2055116919892094 |
| _version_ | 1828759758464614400 |
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| author | Anna Szilasi Lilla Dénes Eszter Krikó Kristin Heenemann Reinhard Ertl Míra Mándoki Thomas W Vahlenkamp Gyula Balka |
| author_facet | Anna Szilasi Lilla Dénes Eszter Krikó Kristin Heenemann Reinhard Ertl Míra Mándoki Thomas W Vahlenkamp Gyula Balka |
| author_sort | Anna Szilasi |
| collection | DOAJ |
| description | Objectives Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting cats worldwide. The objectives of the study were to estimate the prevalence of these retroviruses in domestic cats in Hungary and to characterise the phylogenetic relationships of FIV strains. Methods A total of 335 anticoagulated whole-blood samples obtained from both a healthy and ill cat population were examined for the presence of FIV and FeLV with two methods: ELISA and PCR. Statistical analysis was carried out to analyse the data obtained. Sequencing and phylogenetic analysis of partial polymerase ( pol ) gene sequences was performed to describe circulating FIV subtypes. Results Statistical analysis showed 11.8% and 9.9% true prevalence of FeLV and FIV, respectively, with ELISA. The apparent prevalence calculated from the PCR results were 17.3% for FeLV and 13.1% for FIV. Phylogenetic analysis of partial pol gene sequences obtained from 22 FIV strains showed that all observed Hungarian strains belonged to FIV subtype B. The strains were grouped into several monophyletic subgroups reflecting the geographic locations of the origin of the samples. The overall mean genetic similarity between the analysed strains was 98.2%. Conclusions and relevance We report the first thorough overview of the prevalence of FeLV and FIV in Hungary, which is relatively high, and give insight into the genetic diversity of Hungarian strains of FIV. |
| first_indexed | 2024-12-11T01:02:10Z |
| format | Article |
| id | doaj.art-fd785d89d23d42c0bc71f78057572a25 |
| institution | Directory Open Access Journal |
| issn | 2055-1169 |
| language | English |
| last_indexed | 2024-12-11T01:02:10Z |
| publishDate | 2019-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Feline Medicine and Surgery Open Reports |
| spelling | doaj.art-fd785d89d23d42c0bc71f78057572a252022-12-22T01:26:17ZengSAGE PublishingJournal of Feline Medicine and Surgery Open Reports2055-11692019-12-01510.1177/2055116919892094Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in HungaryAnna Szilasi0Lilla Dénes1Eszter Krikó2Kristin Heenemann3Reinhard Ertl4Míra Mándoki5Thomas W Vahlenkamp6Gyula Balka7Department of Pathology, University of Veterinary Medicine, Budapest, Pest, HungaryDepartment of Pathology, University of Veterinary Medicine, Budapest, Pest, HungaryCentre for Bioinformatics, University of Veterinary Medicine, Budapest, Pest, HungaryInstitute of Virology, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Sachsen, GermanyVetCore Facility for Research, University of Veterinary Medicine, Vienna, AustriaDepartment of Pathology, University of Veterinary Medicine, Budapest, Pest, HungaryInstitute of Virology, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Sachsen, GermanyDepartment of Pathology, University of Veterinary Medicine, Budapest, Pest, HungaryObjectives Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are retroviruses affecting cats worldwide. The objectives of the study were to estimate the prevalence of these retroviruses in domestic cats in Hungary and to characterise the phylogenetic relationships of FIV strains. Methods A total of 335 anticoagulated whole-blood samples obtained from both a healthy and ill cat population were examined for the presence of FIV and FeLV with two methods: ELISA and PCR. Statistical analysis was carried out to analyse the data obtained. Sequencing and phylogenetic analysis of partial polymerase ( pol ) gene sequences was performed to describe circulating FIV subtypes. Results Statistical analysis showed 11.8% and 9.9% true prevalence of FeLV and FIV, respectively, with ELISA. The apparent prevalence calculated from the PCR results were 17.3% for FeLV and 13.1% for FIV. Phylogenetic analysis of partial pol gene sequences obtained from 22 FIV strains showed that all observed Hungarian strains belonged to FIV subtype B. The strains were grouped into several monophyletic subgroups reflecting the geographic locations of the origin of the samples. The overall mean genetic similarity between the analysed strains was 98.2%. Conclusions and relevance We report the first thorough overview of the prevalence of FeLV and FIV in Hungary, which is relatively high, and give insight into the genetic diversity of Hungarian strains of FIV.https://doi.org/10.1177/2055116919892094 |
| spellingShingle | Anna Szilasi Lilla Dénes Eszter Krikó Kristin Heenemann Reinhard Ertl Míra Mándoki Thomas W Vahlenkamp Gyula Balka Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary Journal of Feline Medicine and Surgery Open Reports |
| title | Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary |
| title_full | Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary |
| title_fullStr | Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary |
| title_full_unstemmed | Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary |
| title_short | Prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in Hungary |
| title_sort | prevalence of feline immunodeficiency virus and feline leukaemia virus in domestic cats in hungary |
| url | https://doi.org/10.1177/2055116919892094 |
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