| Summary: | <i>Salmonella</i> can cause serious foodborne diseases. We have developed a lateral flow immunoassay combined with recombinase polymerase amplification (LFD-RPA) for detection of <i>Salmonella</i> in food. The conserved fragment (<i>fimY</i>) was selected as the target gene. Under an optimal condition (37 °C, 10 min), the sensitivity was 12 colony-forming units (CFU)/mL in a pure culture. Testing with 16 non-<i>Salmonella</i> strains as controls revealed that LFD-RPA was specific to the <i>fimY</i> gene of <i>Salmonella</i>. The established assay could detect <i>Salmonella</i> at concentrations as low as 1.29 × 10<sup>2</sup> CFU/mL in artificially contaminated samples. This detection was at a slightly higher level than that for a pure bacterial culture. Combined with the test strip reader, the LFD-RPA is a feasible method for quantitative detection of <i>Salmonella</i> based on the test line intensity, which was the ratio for the test line and control line of the reflected light. The method could be a potential point-of-care test in limited resource areas and provides a new approach and technical support for the diagnosis of food safety.
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