Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection
The necrotrophic fungus Botrytis cinerea is a major threat to grapevine cultivation worldwide. A screen of 41 Vitis genotypes for leaf resistance to B. cinerea suggested species independent variation and revealed eighteen resistant Chinese wild Vitis genotypes, while most investigated V. vinifera, o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00854/full |
| _version_ | 1819177297539760128 |
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| author | Ran eWan Ran eWan Xiaoqing eHou Xiaoqing eHou Xianhang eWang Xianhang eWang Jingwu eQu Jingwu eQu Yuejin eWang Yuejin eWang Xiping eWang Xiping eWang Stacy D. Singer |
| author_facet | Ran eWan Ran eWan Xiaoqing eHou Xiaoqing eHou Xianhang eWang Xianhang eWang Jingwu eQu Jingwu eQu Yuejin eWang Yuejin eWang Xiping eWang Xiping eWang Stacy D. Singer |
| author_sort | Ran eWan |
| collection | DOAJ |
| description | The necrotrophic fungus Botrytis cinerea is a major threat to grapevine cultivation worldwide. A screen of 41 Vitis genotypes for leaf resistance to B. cinerea suggested species independent variation and revealed eighteen resistant Chinese wild Vitis genotypes, while most investigated V. vinifera, or its hybrids, were susceptible. A particularly resistant Chinese wild Vitis, ‘Pingli-5’ (V. sp. [Qinling grape]) and a very susceptible V. vinifera cultivar, ‘Red Globe’ were selected for further study. Microscopic analysis demonstrated that B. cinerea growth was limitted during early infection on ‘Pingli-5’ before 24 hours post inoculation (hpi) but not on Red Globe. It was found that reactive oxygen species (ROS) and antioxidative system were associated with fungal growth. O2- accumulated similarly in B. cinerea 4 hpi on both Vitis genotypes. Lower levels of O2- (not H2O2) were detected 4 hpi and ROS (H2O2 and O2-) accumulation from 8 hpi onwards was also lower in ‘Pingli-5’ leaves than in ‘Red Globe’ leaves. B. cinerea triggered sustained ROS production in ‘Red Globe’ but not in ‘Pingli-5’ with subsequent infection progresses. Red Globe displayed little change in antioxidative activities in response to B. cinerea infection, instead, antioxidative activities were highly and timely elevated in resistant ‘Pingli-5’ which correlated with its minimal ROS increases and its high resistance. These findings not only enhance our understanding of the resistance of Chinese wild Vitis species to B. cinerea, but also lay the foundation for breeding B. cinerea resistant grapes in the future. |
| first_indexed | 2024-12-22T21:24:25Z |
| format | Article |
| id | doaj.art-fd7a987f8f284a2196e254183c827251 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T21:24:25Z |
| publishDate | 2015-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fd7a987f8f284a2196e254183c8272512022-12-21T18:12:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-10-01610.3389/fpls.2015.00854160149Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infectionRan eWan0Ran eWan1Xiaoqing eHou2Xiaoqing eHou3Xianhang eWang4Xianhang eWang5Jingwu eQu6Jingwu eQu7Yuejin eWang8Yuejin eWang9Xiping eWang10Xiping eWang11Stacy D. Singer12State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityKey Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityKey Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityKey Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityKey Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityKey Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F UniversityKey Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F UniversityUniversity of AlbertaThe necrotrophic fungus Botrytis cinerea is a major threat to grapevine cultivation worldwide. A screen of 41 Vitis genotypes for leaf resistance to B. cinerea suggested species independent variation and revealed eighteen resistant Chinese wild Vitis genotypes, while most investigated V. vinifera, or its hybrids, were susceptible. A particularly resistant Chinese wild Vitis, ‘Pingli-5’ (V. sp. [Qinling grape]) and a very susceptible V. vinifera cultivar, ‘Red Globe’ were selected for further study. Microscopic analysis demonstrated that B. cinerea growth was limitted during early infection on ‘Pingli-5’ before 24 hours post inoculation (hpi) but not on Red Globe. It was found that reactive oxygen species (ROS) and antioxidative system were associated with fungal growth. O2- accumulated similarly in B. cinerea 4 hpi on both Vitis genotypes. Lower levels of O2- (not H2O2) were detected 4 hpi and ROS (H2O2 and O2-) accumulation from 8 hpi onwards was also lower in ‘Pingli-5’ leaves than in ‘Red Globe’ leaves. B. cinerea triggered sustained ROS production in ‘Red Globe’ but not in ‘Pingli-5’ with subsequent infection progresses. Red Globe displayed little change in antioxidative activities in response to B. cinerea infection, instead, antioxidative activities were highly and timely elevated in resistant ‘Pingli-5’ which correlated with its minimal ROS increases and its high resistance. These findings not only enhance our understanding of the resistance of Chinese wild Vitis species to B. cinerea, but also lay the foundation for breeding B. cinerea resistant grapes in the future.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00854/fullROSAntioxidative systemResistance evaluationChinese wild VitisBotrytis-Vitis interactions |
| spellingShingle | Ran eWan Ran eWan Xiaoqing eHou Xiaoqing eHou Xianhang eWang Xianhang eWang Jingwu eQu Jingwu eQu Yuejin eWang Yuejin eWang Xiping eWang Xiping eWang Stacy D. Singer Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection Frontiers in Plant Science ROS Antioxidative system Resistance evaluation Chinese wild Vitis Botrytis-Vitis interactions |
| title | Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection |
| title_full | Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection |
| title_fullStr | Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection |
| title_full_unstemmed | Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection |
| title_short | Resistance evaluation of Chinese wild Vitis genotypes against Botrytis cinerea and different responses of resistant and susceptible hosts to the infection |
| title_sort | resistance evaluation of chinese wild vitis genotypes against botrytis cinerea and different responses of resistant and susceptible hosts to the infection |
| topic | ROS Antioxidative system Resistance evaluation Chinese wild Vitis Botrytis-Vitis interactions |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00854/full |
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