Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training
The aim of this study was to evaluate and compare the effectiveness of three concentrations of gentian violet (0.5%, 0.1% and 0.05%) for staining the anterior capsule of the lens in horses. Thirty-six post-mortem equine eyes were collected. The eyes were subdivided into three groups composed of 12...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2023-08-01
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| Series: | Ciência Animal Brasileira |
| Online Access: | https://revistas.ufg.br/vet/article/view/76196 |
| _version_ | 1797733310639636480 |
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| author | Marcelle Bettio Maiara Poersch Seibel Maria Eduarda Mattos Franceschini Rafaella Silva Rocha Renata Lima Baptista Anita Marchionatti Pigatto João Antonio Tadeu Pigatto |
| author_facet | Marcelle Bettio Maiara Poersch Seibel Maria Eduarda Mattos Franceschini Rafaella Silva Rocha Renata Lima Baptista Anita Marchionatti Pigatto João Antonio Tadeu Pigatto |
| author_sort | Marcelle Bettio |
| collection | DOAJ |
| description |
The aim of this study was to evaluate and compare the effectiveness of three concentrations of gentian violet (0.5%, 0.1% and 0.05%) for staining the anterior capsule of the lens in horses. Thirty-six post-mortem equine eyes were collected. The eyes were subdivided into three groups composed of 12 eyes each, according to the concentration of gentian violet used. The effectiveness of staining the anterior capsule of the lens with different concentrations of gentian violet was assessed using an empirical system of evaluation on adequate or inadequate staining of capsular flaps. Based on the evaluation of the examiner, the 0.1% and 0.05% concentrations of gentian violet allowed adequate visualisation of the anterior capsule for continuous curvilinear capsulotomy training, whereas the 0.5% concentration produced strong and inadequate capsular staining. The model developed using gentian violet at concentrations of 0.1% and 0.05% allowed a clear visualisation of the capsular flap, which makes it viable as a model for training the continuous curvilinear capsulotomy step in cataract surgery in horses.
Keywords: anterior capsulotomy; vital dyes; equine; wet lab; surgical training
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| first_indexed | 2024-03-12T12:27:19Z |
| format | Article |
| id | doaj.art-fd7ca1319ccf456692eb060a9c6ed905 |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-03-12T12:27:19Z |
| publishDate | 2023-08-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-fd7ca1319ccf456692eb060a9c6ed9052023-08-29T21:06:18ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912023-08-0124Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical trainingMarcelle Bettio0Maiara Poersch Seibel 1Maria Eduarda Mattos Franceschini 2Rafaella Silva Rocha 3Renata Lima Baptista 4Anita Marchionatti Pigatto 5João Antonio Tadeu Pigatto 6Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, BrasilUniversidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, BrazilUniversidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, BrazilUniversidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, BrazilUniversidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, BrazilUniversidade Estadual Paulista "Júlio de Mesquita Filho (UNESP), Botucatu, São Paulo, BrazilUniversidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil The aim of this study was to evaluate and compare the effectiveness of three concentrations of gentian violet (0.5%, 0.1% and 0.05%) for staining the anterior capsule of the lens in horses. Thirty-six post-mortem equine eyes were collected. The eyes were subdivided into three groups composed of 12 eyes each, according to the concentration of gentian violet used. The effectiveness of staining the anterior capsule of the lens with different concentrations of gentian violet was assessed using an empirical system of evaluation on adequate or inadequate staining of capsular flaps. Based on the evaluation of the examiner, the 0.1% and 0.05% concentrations of gentian violet allowed adequate visualisation of the anterior capsule for continuous curvilinear capsulotomy training, whereas the 0.5% concentration produced strong and inadequate capsular staining. The model developed using gentian violet at concentrations of 0.1% and 0.05% allowed a clear visualisation of the capsular flap, which makes it viable as a model for training the continuous curvilinear capsulotomy step in cataract surgery in horses. Keywords: anterior capsulotomy; vital dyes; equine; wet lab; surgical training https://revistas.ufg.br/vet/article/view/76196 |
| spellingShingle | Marcelle Bettio Maiara Poersch Seibel Maria Eduarda Mattos Franceschini Rafaella Silva Rocha Renata Lima Baptista Anita Marchionatti Pigatto João Antonio Tadeu Pigatto Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training Ciência Animal Brasileira |
| title | Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training |
| title_full | Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training |
| title_fullStr | Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training |
| title_full_unstemmed | Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training |
| title_short | Ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training |
| title_sort | ex vivo evaluation of anterior lens capsule staining in horses with three concentrations of gentian violet for surgical training |
| url | https://revistas.ufg.br/vet/article/view/76196 |
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