Factors affecting gene transformation in mangosteen
Factors affecting gene transformation in mangosteen (Garcinia mangostana L.) were investigated. Types of explants, strains and densities of Agrobacterium tumefaciens, and co-culture methods were examined to optimize gene transformation. The results showed that among strains of Agrobacterium tumefaci...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2003-05-01
|
| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/25-4-pdf/03mangosteen.pdf |
| _version_ | 1819013501053566976 |
|---|---|
| author | Sompong Te-chato Vitoon Chaipakdee |
| author_facet | Sompong Te-chato Vitoon Chaipakdee |
| author_sort | Sompong Te-chato |
| collection | DOAJ |
| description | Factors affecting gene transformation in mangosteen (Garcinia mangostana L.) were investigated. Types of explants, strains and densities of Agrobacterium tumefaciens, and co-culture methods were examined to optimize gene transformation. The results showed that among strains of Agrobacterium tumefaciens tested, LBA 4404 containing pBI 121 gave the calli with the highest resistance to kanamycin. Kanamycin at the concentration of 50-100 mg/l was the best range for selection of transformants. Higher density of agrobacteria tended to promote higher frequency of transformation. The best co-culture method was dipping the explant in a solution of agrobacteria for 10 minutes, followed by culturing onto co-culture medium without antibiotic for 48 hours. Among the explants used to co- culture with bacteria, half leaf treatment gave the best result for transformation; however, callus proliferation and plantlet regeneration were inferior to whole leaf treatment. Activity of β-Glucuronidase (GUS) could not be detected, thus resistance to kanamycin was used for detecting transformability. Shoot primordia could be induced from kanamycin-resistant calli grown in regeneration medium. After maintenance by subculturing to the same medium 2 to 3 times in 2-3 months, the developed shoots turned brown and finally died. Hence, the transformed plant of mangosteen was not obtained from this experiment. |
| first_indexed | 2024-12-21T02:00:57Z |
| format | Article |
| id | doaj.art-fd7d5b4beef54c00b8de1e3a77f9fab2 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-21T02:00:57Z |
| publishDate | 2003-05-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-fd7d5b4beef54c00b8de1e3a77f9fab22022-12-21T19:19:38ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952003-05-01254435449Factors affecting gene transformation in mangosteenSompong Te-chatoVitoon ChaipakdeeFactors affecting gene transformation in mangosteen (Garcinia mangostana L.) were investigated. Types of explants, strains and densities of Agrobacterium tumefaciens, and co-culture methods were examined to optimize gene transformation. The results showed that among strains of Agrobacterium tumefaciens tested, LBA 4404 containing pBI 121 gave the calli with the highest resistance to kanamycin. Kanamycin at the concentration of 50-100 mg/l was the best range for selection of transformants. Higher density of agrobacteria tended to promote higher frequency of transformation. The best co-culture method was dipping the explant in a solution of agrobacteria for 10 minutes, followed by culturing onto co-culture medium without antibiotic for 48 hours. Among the explants used to co- culture with bacteria, half leaf treatment gave the best result for transformation; however, callus proliferation and plantlet regeneration were inferior to whole leaf treatment. Activity of β-Glucuronidase (GUS) could not be detected, thus resistance to kanamycin was used for detecting transformability. Shoot primordia could be induced from kanamycin-resistant calli grown in regeneration medium. After maintenance by subculturing to the same medium 2 to 3 times in 2-3 months, the developed shoots turned brown and finally died. Hence, the transformed plant of mangosteen was not obtained from this experiment.http://www.sjst.psu.ac.th/journal/25-4-pdf/03mangosteen.pdfmangosteengene transformationkanamycinβ-Glucuronidase |
| spellingShingle | Sompong Te-chato Vitoon Chaipakdee Factors affecting gene transformation in mangosteen Songklanakarin Journal of Science and Technology (SJST) mangosteen gene transformation kanamycin β-Glucuronidase |
| title | Factors affecting gene transformation in mangosteen |
| title_full | Factors affecting gene transformation in mangosteen |
| title_fullStr | Factors affecting gene transformation in mangosteen |
| title_full_unstemmed | Factors affecting gene transformation in mangosteen |
| title_short | Factors affecting gene transformation in mangosteen |
| title_sort | factors affecting gene transformation in mangosteen |
| topic | mangosteen gene transformation kanamycin β-Glucuronidase |
| url | http://www.sjst.psu.ac.th/journal/25-4-pdf/03mangosteen.pdf |
| work_keys_str_mv | AT sompongtechato factorsaffectinggenetransformationinmangosteen AT vitoonchaipakdee factorsaffectinggenetransformationinmangosteen |