Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats
Objective(s): The physical properties of nerve guidance channel and components of the regenerating microenvironment can significantly enhance regeneration. The aim of this research was to evaluate the effect of embryo cerebrospinal fluid (ECSF) in nerve regeneration across the microwave irradiated c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Aras Part Medical International Press
2017-10-01
|
| Series: | Crescent Journal of Medical and Biological Sciences |
| Subjects: | |
| Online Access: | http://cjmb.org/uploads/pdf/pdf_CJMB_52.pdf |
| _version_ | 1818001669162532864 |
|---|---|
| author | Seyyed Mostafa Ghavami Gholam Hossein Farjah Hamed Shoorei Mohammad Amin Dolatkhah |
| author_facet | Seyyed Mostafa Ghavami Gholam Hossein Farjah Hamed Shoorei Mohammad Amin Dolatkhah |
| author_sort | Seyyed Mostafa Ghavami |
| collection | DOAJ |
| description | Objective(s): The physical properties of nerve guidance channel and components of the regenerating microenvironment can significantly enhance regeneration. The aim of this research was to evaluate the effect of embryo cerebrospinal fluid (ECSF) in nerve regeneration across the microwave irradiated collagen nerve guides in comparison with autograft.
Material and Methods: Under general anesthesia, the left sciatic nerve was exposed and 10 mm nerve segment defect was created in 40 adult male Sprague-Dawley rats (250-300 g). Animals were randomly divided into 4 experimental groups: repair with reversed autograft, reconstruction with collagen nerve conduit filled with ECSF, reconstruction with collagen nerve conduit filled with normal saline (NS) and sham surgery. All animals were evaluated by sciatic functional index (SFI), electrophysiology, and histopathological staining at weeks 4 and 12 after surgery.
Results: The mean SFI value of group collagen + ECSF and autograft was significantly higher than that of group NS on days 49 and 60 post-operation (P < 0.05). After 90 days after the operation, the mean nerve conduction velocity (NCV) of groups collagen + ECSF and autograft were significantly faster than NS group (P < 0.05). The regenerated nerves of groups collagen + ECSF and autograft were more mature than that of the group NS group at day 90 (P < 0.05). There was no significant difference between groups collagen + ECSF and autograft.
Conclusion: These findings showed that chick CSF in collagen guide can enhance nerve regeneration and promote functional recovery in the injured sciatic nerve of rats. |
| first_indexed | 2024-04-14T03:37:16Z |
| format | Article |
| id | doaj.art-fd80fdbd2fdf4ad9a533f1c6084d8ccc |
| institution | Directory Open Access Journal |
| issn | 2148-9696 2148-9696 |
| language | English |
| last_indexed | 2024-04-14T03:37:16Z |
| publishDate | 2017-10-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-fd80fdbd2fdf4ad9a533f1c6084d8ccc2022-12-22T02:14:43ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962148-96962017-10-0144194199Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in RatsSeyyed Mostafa Ghavami0Gholam Hossein Farjah1 Hamed Shoorei2Mohammad Amin Dolatkhah3Department of Radiology, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, IranNeurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IranDepartment of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranObjective(s): The physical properties of nerve guidance channel and components of the regenerating microenvironment can significantly enhance regeneration. The aim of this research was to evaluate the effect of embryo cerebrospinal fluid (ECSF) in nerve regeneration across the microwave irradiated collagen nerve guides in comparison with autograft. Material and Methods: Under general anesthesia, the left sciatic nerve was exposed and 10 mm nerve segment defect was created in 40 adult male Sprague-Dawley rats (250-300 g). Animals were randomly divided into 4 experimental groups: repair with reversed autograft, reconstruction with collagen nerve conduit filled with ECSF, reconstruction with collagen nerve conduit filled with normal saline (NS) and sham surgery. All animals were evaluated by sciatic functional index (SFI), electrophysiology, and histopathological staining at weeks 4 and 12 after surgery. Results: The mean SFI value of group collagen + ECSF and autograft was significantly higher than that of group NS on days 49 and 60 post-operation (P < 0.05). After 90 days after the operation, the mean nerve conduction velocity (NCV) of groups collagen + ECSF and autograft were significantly faster than NS group (P < 0.05). The regenerated nerves of groups collagen + ECSF and autograft were more mature than that of the group NS group at day 90 (P < 0.05). There was no significant difference between groups collagen + ECSF and autograft. Conclusion: These findings showed that chick CSF in collagen guide can enhance nerve regeneration and promote functional recovery in the injured sciatic nerve of rats.http://cjmb.org/uploads/pdf/pdf_CJMB_52.pdfCerebrospinal fluidSciatic nerveCollagenNerve injuryMicrowave |
| spellingShingle | Seyyed Mostafa Ghavami Gholam Hossein Farjah Hamed Shoorei Mohammad Amin Dolatkhah Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats Crescent Journal of Medical and Biological Sciences Cerebrospinal fluid Sciatic nerve Collagen Nerve injury Microwave |
| title | Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats |
| title_full | Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats |
| title_fullStr | Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats |
| title_full_unstemmed | Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats |
| title_short | Functional Recovery of Transected Peripheral Nerve by Means of Microwave Irradiated Collagen Nerve Guides Filled With Chick Embryonic Cerebrospinal Fluid in Rats |
| title_sort | functional recovery of transected peripheral nerve by means of microwave irradiated collagen nerve guides filled with chick embryonic cerebrospinal fluid in rats |
| topic | Cerebrospinal fluid Sciatic nerve Collagen Nerve injury Microwave |
| url | http://cjmb.org/uploads/pdf/pdf_CJMB_52.pdf |
| work_keys_str_mv | AT seyyedmostafaghavami functionalrecoveryoftransectedperipheralnervebymeansofmicrowaveirradiatedcollagennerveguidesfilledwithchickembryoniccerebrospinalfluidinrats AT gholamhosseinfarjah functionalrecoveryoftransectedperipheralnervebymeansofmicrowaveirradiatedcollagennerveguidesfilledwithchickembryoniccerebrospinalfluidinrats AT hamedshoorei functionalrecoveryoftransectedperipheralnervebymeansofmicrowaveirradiatedcollagennerveguidesfilledwithchickembryoniccerebrospinalfluidinrats AT mohammadamindolatkhah functionalrecoveryoftransectedperipheralnervebymeansofmicrowaveirradiatedcollagennerveguidesfilledwithchickembryoniccerebrospinalfluidinrats |