Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing
Down syndrome cell adhesion molecule (Dscam) gene encodes a cell adhesion molecule required for neuronal wiring. A remarkable feature of arthropod Dscam is massive alternative splicing generating thousands of different isoforms from three variable clusters of alternative exons. Dscam expression and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1322808/full |
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| author | Pinar Ustaoglu Pinar Ustaoglu David W. J. McQuarrie David W. J. McQuarrie Anthony Rochet Thomas C. Dix Thomas C. Dix Irmgard U. Haussmann Irmgard U. Haussmann Roland Arnold Roland Arnold Jean-Marc Devaud Jean-Marc Devaud Matthias Soller Matthias Soller |
| author_facet | Pinar Ustaoglu Pinar Ustaoglu David W. J. McQuarrie David W. J. McQuarrie Anthony Rochet Thomas C. Dix Thomas C. Dix Irmgard U. Haussmann Irmgard U. Haussmann Roland Arnold Roland Arnold Jean-Marc Devaud Jean-Marc Devaud Matthias Soller Matthias Soller |
| author_sort | Pinar Ustaoglu |
| collection | DOAJ |
| description | Down syndrome cell adhesion molecule (Dscam) gene encodes a cell adhesion molecule required for neuronal wiring. A remarkable feature of arthropod Dscam is massive alternative splicing generating thousands of different isoforms from three variable clusters of alternative exons. Dscam expression and diversity arising from alternative splicing have been studied during development, but whether they exert functions in adult brains has not been determined. Here, using honey bees, we find that Dscam expression is critically linked to memory retention as reducing expression by RNAi enhances memory after reward learning in adult worker honey bees. Moreover, alternative splicing of Dscam is altered in all three variable clusters after learning. Since identical Dscam isoforms engage in homophilic interactions, these results suggest a mechanism to alter inclusion of variable exons during memory consolidation to modify neuronal connections for memory retention. |
| first_indexed | 2024-03-08T15:53:14Z |
| format | Article |
| id | doaj.art-fd81551ee6684cafa512de51b39b6095 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-03-08T15:53:14Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-fd81551ee6684cafa512de51b39b60952024-01-09T04:14:19ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992024-01-011610.3389/fnmol.2023.13228081322808Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicingPinar Ustaoglu0Pinar Ustaoglu1David W. J. McQuarrie2David W. J. McQuarrie3Anthony Rochet4Thomas C. Dix5Thomas C. Dix6Irmgard U. Haussmann7Irmgard U. Haussmann8Roland Arnold9Roland Arnold10Jean-Marc Devaud11Jean-Marc Devaud12Matthias Soller13Matthias Soller14School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, United KingdomBirmingham Centre for Genome Biology, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, United KingdomBirmingham Centre for Genome Biology, University of Birmingham, Birmingham, United KingdomResearch Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), CNRS, UPS, Toulouse University, Toulouse, FranceSchool of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, United KingdomBirmingham Centre for Genome Biology, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, United KingdomDepartment of Life Science, Faculty of Health, Education and Life Sciences, Birmingham City University, Birmingham, United KingdomBirmingham Centre for Genome Biology, University of Birmingham, Birmingham, United KingdomCollege of Medical and Dental Sciences, Institute of Cancer and Genomics Sciences, University of Birmingham, Birmingham, United KingdomResearch Center on Animal Cognition (CRCA), Center for Integrative Biology (CBI), CNRS, UPS, Toulouse University, Toulouse, FranceInstitut Universitaire de France (IUF), Paris, FranceSchool of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, United KingdomBirmingham Centre for Genome Biology, University of Birmingham, Birmingham, United KingdomDown syndrome cell adhesion molecule (Dscam) gene encodes a cell adhesion molecule required for neuronal wiring. A remarkable feature of arthropod Dscam is massive alternative splicing generating thousands of different isoforms from three variable clusters of alternative exons. Dscam expression and diversity arising from alternative splicing have been studied during development, but whether they exert functions in adult brains has not been determined. Here, using honey bees, we find that Dscam expression is critically linked to memory retention as reducing expression by RNAi enhances memory after reward learning in adult worker honey bees. Moreover, alternative splicing of Dscam is altered in all three variable clusters after learning. Since identical Dscam isoforms engage in homophilic interactions, these results suggest a mechanism to alter inclusion of variable exons during memory consolidation to modify neuronal connections for memory retention.https://www.frontiersin.org/articles/10.3389/fnmol.2023.1322808/fullDscamalternative splicinglearning and memoryhoney beeRNA interference (gene silencing) |
| spellingShingle | Pinar Ustaoglu Pinar Ustaoglu David W. J. McQuarrie David W. J. McQuarrie Anthony Rochet Thomas C. Dix Thomas C. Dix Irmgard U. Haussmann Irmgard U. Haussmann Roland Arnold Roland Arnold Jean-Marc Devaud Jean-Marc Devaud Matthias Soller Matthias Soller Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing Frontiers in Molecular Neuroscience Dscam alternative splicing learning and memory honey bee RNA interference (gene silencing) |
| title | Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing |
| title_full | Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing |
| title_fullStr | Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing |
| title_full_unstemmed | Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing |
| title_short | Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing |
| title_sort | memory consolidation in honey bees is enhanced by down regulation of down syndrome cell adhesion molecule and changes its alternative splicing |
| topic | Dscam alternative splicing learning and memory honey bee RNA interference (gene silencing) |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1322808/full |
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