Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood
The molecular detection of <i>Candida</i> plays an important role in the diagnosis of candidaemia, a major cause of morbidity and mortality. The sensitivity of this diagnosis is partly related to the efficiency of yeast DNA extraction. In this monocentric study, we investigated the suita...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/3/228 |
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| author | Estelle Menu Jordi Landier Elsa Prudent Stéphane Ranque Coralie L’Ollivier |
| author_facet | Estelle Menu Jordi Landier Elsa Prudent Stéphane Ranque Coralie L’Ollivier |
| author_sort | Estelle Menu |
| collection | DOAJ |
| description | The molecular detection of <i>Candida</i> plays an important role in the diagnosis of candidaemia, a major cause of morbidity and mortality. The sensitivity of this diagnosis is partly related to the efficiency of yeast DNA extraction. In this monocentric study, we investigated the suitability of 11 recent automated procedures for the extraction of low and high amounts of <i>Candida</i> DNA from spiked blood. The efficacy of the DNA extraction procedures to detect <i>Candida</i> spp. in blood samples ranged from 31.4% to 80.6%. The NucliSENS<sup>TM</sup> easyMAG<sup>TM</sup> procedure was the most efficient, for each species and each inoculum. It significantly outperformed the other procedures at the lower <i>Candida</i> inocula mimicking the clinical setting. This study highlighted a heterogeneity in DNA extraction efficacy between the five main <i>Candida</i> species (<i>Candida albicans</i>, <i>Candida glabrata</i>, <i>Candida parapsilosis</i>, <i>Candida tropicalis</i> and <i>Candida krusei</i>). Up to five automated procedures were appropriate for <i>C. krusei</i> DNA extraction, whereas only one method yielded an appropriate detection of low amount of <i>C. tropicalis</i>. In the era of the syndromic approach to bloodstream infection diagnosis, this evaluation of 11 automated DNA extraction methods for the PCR diagnosis of candidaemia, puts the choice of an appropriate method in routine diagnosis within the reach of laboratories. |
| first_indexed | 2024-03-10T13:04:25Z |
| format | Article |
| id | doaj.art-fd81bbbf73b442229e088478d162d505 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T13:04:25Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-fd81bbbf73b442229e088478d162d5052023-11-21T11:15:45ZengMDPI AGJournal of Fungi2309-608X2021-03-017322810.3390/jof7030228Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked BloodEstelle Menu0Jordi Landier1Elsa Prudent2Stéphane Ranque3Coralie L’Ollivier4Institut de Recherche pour le Développement, Aix Marseille Université, Assistance Publique-Hôpitaux de Marseille, Service de Santé des Armées, VITROME: Vecteurs—Infections Tropicales et Méditerranéennes, 13385 Marseille, FranceSciences Economiques & Sociales de la Santé & Traitement de l’Information Médicale, Institut de Recherche pour le Développement, Institut National de Santé et de Recherche Médicale, Aix Marseille Université, 13385 Marseille, FranceIHU Méditerranée Infection, 13385 Marseille, FranceInstitut de Recherche pour le Développement, Aix Marseille Université, Assistance Publique-Hôpitaux de Marseille, Service de Santé des Armées, VITROME: Vecteurs—Infections Tropicales et Méditerranéennes, 13385 Marseille, FranceInstitut de Recherche pour le Développement, Aix Marseille Université, Assistance Publique-Hôpitaux de Marseille, Service de Santé des Armées, VITROME: Vecteurs—Infections Tropicales et Méditerranéennes, 13385 Marseille, FranceThe molecular detection of <i>Candida</i> plays an important role in the diagnosis of candidaemia, a major cause of morbidity and mortality. The sensitivity of this diagnosis is partly related to the efficiency of yeast DNA extraction. In this monocentric study, we investigated the suitability of 11 recent automated procedures for the extraction of low and high amounts of <i>Candida</i> DNA from spiked blood. The efficacy of the DNA extraction procedures to detect <i>Candida</i> spp. in blood samples ranged from 31.4% to 80.6%. The NucliSENS<sup>TM</sup> easyMAG<sup>TM</sup> procedure was the most efficient, for each species and each inoculum. It significantly outperformed the other procedures at the lower <i>Candida</i> inocula mimicking the clinical setting. This study highlighted a heterogeneity in DNA extraction efficacy between the five main <i>Candida</i> species (<i>Candida albicans</i>, <i>Candida glabrata</i>, <i>Candida parapsilosis</i>, <i>Candida tropicalis</i> and <i>Candida krusei</i>). Up to five automated procedures were appropriate for <i>C. krusei</i> DNA extraction, whereas only one method yielded an appropriate detection of low amount of <i>C. tropicalis</i>. In the era of the syndromic approach to bloodstream infection diagnosis, this evaluation of 11 automated DNA extraction methods for the PCR diagnosis of candidaemia, puts the choice of an appropriate method in routine diagnosis within the reach of laboratories.https://www.mdpi.com/2309-608X/7/3/228<i>Candida</i>candidaemiaDNA extraction |
| spellingShingle | Estelle Menu Jordi Landier Elsa Prudent Stéphane Ranque Coralie L’Ollivier Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood Journal of Fungi <i>Candida</i> candidaemia DNA extraction |
| title | Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood |
| title_full | Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood |
| title_fullStr | Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood |
| title_full_unstemmed | Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood |
| title_short | Evaluation of 11 DNA Automated Extraction Protocols for the Detection of the 5 Mains Candida Species from Artificially Spiked Blood |
| title_sort | evaluation of 11 dna automated extraction protocols for the detection of the 5 mains candida species from artificially spiked blood |
| topic | <i>Candida</i> candidaemia DNA extraction |
| url | https://www.mdpi.com/2309-608X/7/3/228 |
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