Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs.
One hallmark of pathogenic yersiniae is their ability to rapidly adjust their life-style and pathogenesis upon host entry. In order to capture the range, magnitude and complexity of the underlying gene control mechanisms we used comparative RNA-seq-based transcriptomic profiling of the enteric patho...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-03-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC4376681?pdf=render |
| _version_ | 1811334846135402496 |
|---|---|
| author | Aaron M Nuss Ann Kathrin Heroven Barbara Waldmann Jan Reinkensmeier Michael Jarek Michael Beckstette Petra Dersch |
| author_facet | Aaron M Nuss Ann Kathrin Heroven Barbara Waldmann Jan Reinkensmeier Michael Jarek Michael Beckstette Petra Dersch |
| author_sort | Aaron M Nuss |
| collection | DOAJ |
| description | One hallmark of pathogenic yersiniae is their ability to rapidly adjust their life-style and pathogenesis upon host entry. In order to capture the range, magnitude and complexity of the underlying gene control mechanisms we used comparative RNA-seq-based transcriptomic profiling of the enteric pathogen Y. pseudotuberculosis under environmental and infection-relevant conditions. We identified 1151 individual transcription start sites, multiple riboswitch-like RNA elements, and a global set of antisense RNAs and previously unrecognized trans-acting RNAs. Taking advantage of these data, we revealed a temperature-induced and growth phase-dependent reprogramming of a large set of catabolic/energy production genes and uncovered the existence of a thermo-regulated 'acetate switch', which appear to prime the bacteria for growth in the digestive tract. To elucidate the regulatory architecture linking nutritional status to virulence we also refined the CRP regulon. We identified a massive remodelling of the CRP-controlled network in response to temperature and discovered CRP as a transcriptional master regulator of numerous conserved and newly identified non-coding RNAs which participate in this process. This finding highlights a novel level of complexity of the regulatory network in which the concerted action of transcriptional regulators and multiple non-coding RNAs under control of CRP adjusts the control of Yersinia fitness and virulence to the requirements of their environmental and virulent life-styles. |
| first_indexed | 2024-04-13T17:15:14Z |
| format | Article |
| id | doaj.art-fd840d934fd64fbd9b4a6223124ab09c |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T17:15:14Z |
| publishDate | 2015-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-fd840d934fd64fbd9b4a6223124ab09c2022-12-22T02:38:10ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-03-01113e100508710.1371/journal.pgen.1005087Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs.Aaron M NussAnn Kathrin HerovenBarbara WaldmannJan ReinkensmeierMichael JarekMichael BeckstettePetra DerschOne hallmark of pathogenic yersiniae is their ability to rapidly adjust their life-style and pathogenesis upon host entry. In order to capture the range, magnitude and complexity of the underlying gene control mechanisms we used comparative RNA-seq-based transcriptomic profiling of the enteric pathogen Y. pseudotuberculosis under environmental and infection-relevant conditions. We identified 1151 individual transcription start sites, multiple riboswitch-like RNA elements, and a global set of antisense RNAs and previously unrecognized trans-acting RNAs. Taking advantage of these data, we revealed a temperature-induced and growth phase-dependent reprogramming of a large set of catabolic/energy production genes and uncovered the existence of a thermo-regulated 'acetate switch', which appear to prime the bacteria for growth in the digestive tract. To elucidate the regulatory architecture linking nutritional status to virulence we also refined the CRP regulon. We identified a massive remodelling of the CRP-controlled network in response to temperature and discovered CRP as a transcriptional master regulator of numerous conserved and newly identified non-coding RNAs which participate in this process. This finding highlights a novel level of complexity of the regulatory network in which the concerted action of transcriptional regulators and multiple non-coding RNAs under control of CRP adjusts the control of Yersinia fitness and virulence to the requirements of their environmental and virulent life-styles.http://europepmc.org/articles/PMC4376681?pdf=render |
| spellingShingle | Aaron M Nuss Ann Kathrin Heroven Barbara Waldmann Jan Reinkensmeier Michael Jarek Michael Beckstette Petra Dersch Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs. PLoS Genetics |
| title | Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs. |
| title_full | Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs. |
| title_fullStr | Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs. |
| title_full_unstemmed | Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs. |
| title_short | Transcriptomic profiling of Yersinia pseudotuberculosis reveals reprogramming of the Crp regulon by temperature and uncovers Crp as a master regulator of small RNAs. |
| title_sort | transcriptomic profiling of yersinia pseudotuberculosis reveals reprogramming of the crp regulon by temperature and uncovers crp as a master regulator of small rnas |
| url | http://europepmc.org/articles/PMC4376681?pdf=render |
| work_keys_str_mv | AT aaronmnuss transcriptomicprofilingofyersiniapseudotuberculosisrevealsreprogrammingofthecrpregulonbytemperatureanduncoverscrpasamasterregulatorofsmallrnas AT annkathrinheroven transcriptomicprofilingofyersiniapseudotuberculosisrevealsreprogrammingofthecrpregulonbytemperatureanduncoverscrpasamasterregulatorofsmallrnas AT barbarawaldmann transcriptomicprofilingofyersiniapseudotuberculosisrevealsreprogrammingofthecrpregulonbytemperatureanduncoverscrpasamasterregulatorofsmallrnas AT janreinkensmeier transcriptomicprofilingofyersiniapseudotuberculosisrevealsreprogrammingofthecrpregulonbytemperatureanduncoverscrpasamasterregulatorofsmallrnas AT michaeljarek transcriptomicprofilingofyersiniapseudotuberculosisrevealsreprogrammingofthecrpregulonbytemperatureanduncoverscrpasamasterregulatorofsmallrnas AT michaelbeckstette transcriptomicprofilingofyersiniapseudotuberculosisrevealsreprogrammingofthecrpregulonbytemperatureanduncoverscrpasamasterregulatorofsmallrnas AT petradersch transcriptomicprofilingofyersiniapseudotuberculosisrevealsreprogrammingofthecrpregulonbytemperatureanduncoverscrpasamasterregulatorofsmallrnas |