Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV)
Nucleotide-binding leucine-rich repeat (NLR) plant immune receptors mediate the recognition and activation of defense signaling pathways in response to intra- and extracellular pathogens. Several NLR such as Tm-2 and Tm-22 have been introgressed into commercial solanaceous varieties to confer protec...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.984846/full |
| _version_ | 1811253905131044864 |
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| author | Karla Rivera-Márquez Leandro Alberto Núñez-Muñoz Leandro Alberto Núñez-Muñoz Berenice Calderón-Pérez Rodolfo De La Torre-Almaraz Brenda Yazmín Vargas-Hernández Roberto Ruiz-Medrano Beatriz Xoconostle-Cázares |
| author_facet | Karla Rivera-Márquez Leandro Alberto Núñez-Muñoz Leandro Alberto Núñez-Muñoz Berenice Calderón-Pérez Rodolfo De La Torre-Almaraz Brenda Yazmín Vargas-Hernández Roberto Ruiz-Medrano Beatriz Xoconostle-Cázares |
| author_sort | Karla Rivera-Márquez |
| collection | DOAJ |
| description | Nucleotide-binding leucine-rich repeat (NLR) plant immune receptors mediate the recognition and activation of defense signaling pathways in response to intra- and extracellular pathogens. Several NLR such as Tm-2 and Tm-22 have been introgressed into commercial solanaceous varieties to confer protection against different tobamoviruses. Particularly, Tm-22 was used during recent decades to confer resistance against tobacco mosaic virus, tomato mottle mosaic virus and tomato mosaic virus, which recognizes the viral movement protein (MP). However, tomato brown rugose fruit virus(ToBRFV), a novel tobamovirus, can avoid the protection conferred by Tm-22 due to the presence of key substitutions in the MP. The aim of this work was to identify the key amino acid residues involved in the interaction between Tm-22 and ToBRFV MP through bioinformatic analyses, and to identify potential Tm-22 mutations that could generate greater binding affinity. In silico 3D structure prediction, molecular docking, and computational affinity methods were performed. We predicted that R350, H384 and K385 Tm-22 residues are relevant for the interaction with MP, and two mutations (H384W and K385L) were identified as putative sites to increase the affinity of Tm-22 to the MP with the potential elicitation of resistance against ToBRFV. |
| first_indexed | 2024-04-12T16:59:05Z |
| format | Article |
| id | doaj.art-fd85bdbdcde14b37a35961ba609fb796 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T16:59:05Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-fd85bdbdcde14b37a35961ba609fb7962022-12-22T03:24:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.984846984846Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV)Karla Rivera-Márquez0Leandro Alberto Núñez-Muñoz1Leandro Alberto Núñez-Muñoz2Berenice Calderón-Pérez3Rodolfo De La Torre-Almaraz4Brenda Yazmín Vargas-Hernández5Roberto Ruiz-Medrano6Beatriz Xoconostle-Cázares7Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Mexico City, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Mexico City, MexicoUnidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Mexico, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Mexico City, MexicoUnidad de Biotecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Mexico, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Mexico City, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Mexico City, MexicoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Mexico City, MexicoNucleotide-binding leucine-rich repeat (NLR) plant immune receptors mediate the recognition and activation of defense signaling pathways in response to intra- and extracellular pathogens. Several NLR such as Tm-2 and Tm-22 have been introgressed into commercial solanaceous varieties to confer protection against different tobamoviruses. Particularly, Tm-22 was used during recent decades to confer resistance against tobacco mosaic virus, tomato mottle mosaic virus and tomato mosaic virus, which recognizes the viral movement protein (MP). However, tomato brown rugose fruit virus(ToBRFV), a novel tobamovirus, can avoid the protection conferred by Tm-22 due to the presence of key substitutions in the MP. The aim of this work was to identify the key amino acid residues involved in the interaction between Tm-22 and ToBRFV MP through bioinformatic analyses, and to identify potential Tm-22 mutations that could generate greater binding affinity. In silico 3D structure prediction, molecular docking, and computational affinity methods were performed. We predicted that R350, H384 and K385 Tm-22 residues are relevant for the interaction with MP, and two mutations (H384W and K385L) were identified as putative sites to increase the affinity of Tm-22 to the MP with the potential elicitation of resistance against ToBRFV.https://www.frontiersin.org/articles/10.3389/fpls.2022.984846/fullToBRFVresistanceTm-22molecular dockingin silico mutagenesis |
| spellingShingle | Karla Rivera-Márquez Leandro Alberto Núñez-Muñoz Leandro Alberto Núñez-Muñoz Berenice Calderón-Pérez Rodolfo De La Torre-Almaraz Brenda Yazmín Vargas-Hernández Roberto Ruiz-Medrano Beatriz Xoconostle-Cázares Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV) Frontiers in Plant Science ToBRFV resistance Tm-22 molecular docking in silico mutagenesis |
| title | Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV) |
| title_full | Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV) |
| title_fullStr | Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV) |
| title_full_unstemmed | Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV) |
| title_short | Bioinformatic-based approach for mutagenesis of plant immune Tm-22 receptor to confer resistance against tomato brown rugose fruit virus (ToBRFV) |
| title_sort | bioinformatic based approach for mutagenesis of plant immune tm 22 receptor to confer resistance against tomato brown rugose fruit virus tobrfv |
| topic | ToBRFV resistance Tm-22 molecular docking in silico mutagenesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.984846/full |
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